{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2024,3,6]],"date-time":"2024-03-06T15:07:56Z","timestamp":1709737676559},"reference-count":18,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2018,3,8]],"date-time":"2018-03-08T00:00:00Z","timestamp":1520467200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"National Institute of Virology (Pune, India)"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Microbiology and Immunology"],"published-print":{"date-parts":[[2018,4]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:sec><jats:label \/><jats:p>Mumps is an infectious disease caused by mumps virus (MuV), which belongs to the family <jats:italic>Paramyxoviridae and<\/jats:italic> genus <jats:italic>Rubulavirus<\/jats:italic>. Typical symptoms of mumps include fever and swelling of the parotid glands; however, mumps can be asymptomatic. Mumps is diagnosed by molecular and serological methods (i.e., PCR and Enzyme Immunoassay [EIA]); however, both methods have pros and cons. This study was performed to compare the diagnostic utility of a focus reduction neutralization test (FRNT) to that of MuV\u2010specific commercial IgM and IgG antibody EIA in patients suspected of having mumps. One hundred\u2010eighty six samples collected during mumps outbreak in 2012\u201316 were studied. Samples (<jats:italic>n<\/jats:italic>\u2009=\u200980) were tested by all the three serological assays and showed 70.4%, 83% and 92.5% positivity by IgM EIA, IgG and FRNT, respectively. In all, 58.8% samples (<jats:italic>n<\/jats:italic>\u2009=\u200947) tested positive in all three assays. Concordance between mumps RT\u2010PCR and IgM EIA was highest during the first 2\u20135 days and decreased with increasing time post\u2010onset. Mumps FRNT results agreed with those of RT\u2010PCR\/IgM EIA from the second week onwards, whereas the results of mumps IgG EIA agreed with those of RT\u2010PCR\/IgM EIA from post\u2010onset days 3\u201310. These findings suggest the utility of a FRNT for laboratory diagnosis of mumps in countries whose populations are not immunized against this infection.<\/jats:p><\/jats:sec>","DOI":"10.1111\/1348-0421.12576","type":"journal-article","created":{"date-parts":[[2018,1,24]],"date-time":"2018-01-24T11:22:39Z","timestamp":1516792959000},"page":"243-247","source":"Crossref","is-referenced-by-count":3,"title":["Utility of neutralization test for laboratory diagnosis of suspected mumps"],"prefix":"10.1111","volume":"62","author":[{"given":"Sunil R.","family":"Vaidya","sequence":"first","affiliation":[{"name":"Indian Council of Medical Research\u2013National Institute of Virology 20\u2010A Dr. Ambedkar Road Pune 411001 India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Venkat S.","family":"Hamde","sequence":"additional","affiliation":[{"name":"Department of Microbiology Yogeshwari Mahavidyalaya Ambajogai District\u2010Beed, 431517 (Maharashtra State) affiliated to Dr Babasaheb Ambedkar Marathwada University Aurangabad 431004 India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Neelakshi S.","family":"Kumbhar","sequence":"additional","affiliation":[{"name":"Indian Council of Medical Research\u2013National Institute of Virology 20\u2010A Dr. Ambedkar Road Pune 411001 India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Atul M.","family":"Walimbe","sequence":"additional","affiliation":[{"name":"Indian Council of Medical Research\u2013National Institute of Virology 20\u2010A Dr. Ambedkar Road Pune 411001 India"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2018,3,8]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1099\/0022-1317-69-11-2893"},{"key":"e_1_2_8_3_1","first-page":"217","article-title":"World Health Organization. (2012) Mumps virus nomenclature update","volume":"87","year":"2012","journal-title":"Wkly Epidemiol Rec"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1007\/s13312-015-0666-2"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1007\/s13312-016-0874-4"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jcv.2014.11.004"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijmm.2015.08.011"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1038\/pr.2015.141"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0463.2006.apm_463.x"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.1111\/1348-0421.12201"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jviromet.2009.09.006"},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.1086\/314957"},{"key":"e_1_2_8_13_1","doi-asserted-by":"publisher","DOI":"10.1002\/jmv.23600"},{"key":"e_1_2_8_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/0166-0934(86)90077-7"},{"key":"e_1_2_8_15_1","doi-asserted-by":"publisher","DOI":"10.1002\/jmv.1890080207"},{"key":"e_1_2_8_16_1","first-page":"16","article-title":"Contribution of the detection of IgA antibodies to the laboratory diagnosis of mumps in the population with a high vaccination coverage","volume":"64","author":"Limberkov\u00e1 R.","year":"2015","journal-title":"Epidemiol Mikrobiol Imunol"},{"key":"e_1_2_8_17_1","doi-asserted-by":"publisher","DOI":"10.1128\/CDLI.5.6.799-803.1998"},{"key":"e_1_2_8_18_1","doi-asserted-by":"publisher","DOI":"10.1093\/eurpub\/ckx067"},{"key":"e_1_2_8_19_1","doi-asserted-by":"publisher","DOI":"10.4103\/0971-5916.178587"}],"container-title":["Microbiology and Immunology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2F1348-0421.12576","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/1348-0421.12576","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,24]],"date-time":"2023-09-24T14:27:42Z","timestamp":1695565662000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/1348-0421.12576"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2018,3,8]]},"references-count":18,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2018,4]]}},"alternative-id":["10.1111\/1348-0421.12576"],"URL":"https:\/\/doi.org\/10.1111\/1348-0421.12576","archive":["Portico"],"relation":{},"ISSN":["0385-5600","1348-0421"],"issn-type":[{"value":"0385-5600","type":"print"},{"value":"1348-0421","type":"electronic"}],"subject":[],"published":{"date-parts":[[2018,3,8]]}}}