{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,1]],"date-time":"2025-11-01T21:45:23Z","timestamp":1762033523494,"version":"3.37.3"},"reference-count":28,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2018,6,12]],"date-time":"2018-06-12T00:00:00Z","timestamp":1528761600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100008982","name":"National Science Foundation","doi-asserted-by":"publisher","award":["NSF\/SCH\/2015\/04"],"award-info":[{"award-number":["NSF\/SCH\/2015\/04"]}],"id":[{"id":"10.13039\/501100008982","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100008816","name":"University of Sri Jayewardenepura","doi-asserted-by":"publisher","award":["ASP\/01\/RE\/MED\/2017\/28"],"award-info":[{"award-number":["ASP\/01\/RE\/MED\/2017\/28"]}],"id":[{"id":"10.13039\/501100008816","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Microbiology and Immunology"],"published-print":{"date-parts":[[2018,7]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:sec><jats:label\/><jats:p>Single nucleotide polymorphisms present on the promoter sequence of the <jats:italic>TNF\u2010\u03b1<\/jats:italic> gene may affect production of <jats:italic>TNF\u2010\u03b1<\/jats:italic>, a pro\u2010inflammatory cytokine, during immune responses. The presence of <jats:italic>TNF\u2010\u03b1<\/jats:italic> polymorphisms is also reportedly associated with more severe manifestations of <jats:italic>Helicobacter pylori<\/jats:italic> infection. However, the frequency of <jats:italic>TNF\u2010\u03b1<\/jats:italic> polymorphisms and the associated disease severity vary between different patient groups. In this study, gastric biopsies and blood specimens were collected from 138 patients with dyspepsia undergoing routine upper gastrointestinal endoscopy. Our institution's Ethics Review Committee approved the study and written informed consent was obtained from all participants. The presence of <jats:italic>H. pylori<\/jats:italic> was confirmed histologically in all patients. The frequency of <jats:italic>TNF\u2010\u03b1<\/jats:italic> polymorphisms in the study cohort was investigated using PCR\u2013restriction fragment length polymorphism and expression of serum <jats:italic>TNF\u2010\u03b1<\/jats:italic> quantitated using a commercial ELISA assay. The proportions of selected <jats:italic>TNF\u2010\u03b1<\/jats:italic> polymorphisms (<jats:italic>TNF\u2010\u03b1 \u2010238<\/jats:italic>, \u2010<jats:italic>308<\/jats:italic> and \u2010<jats:italic>863<\/jats:italic>) were similar in <jats:italic>H. pylori<\/jats:italic>\u2010positive and \u2010negative patients. Homozygous mutations of <jats:italic>TNF\u2010\u03b1<\/jats:italic> polymorphisms were rarely detected in the study group. There was a significant difference in <jats:italic>TNF\u2010\u03b1<\/jats:italic> concentrations between patients with mild chronic gastritis and <jats:italic>TNF\u2010\u03b1 \u2010308 GG<\/jats:italic> genotype and patients with moderate to severe chronic gastritis (<jats:italic>P<\/jats:italic>\u2009=\u20090.008). It was not possible to identify an association between these genotypes and disease severity because of the low frequency of heterozygous and homozygous mutated genes in Sri Lankan patients with dyspepsia.<\/jats:p><\/jats:sec>","DOI":"10.1111\/1348-0421.12597","type":"journal-article","created":{"date-parts":[[2018,4,28]],"date-time":"2018-04-28T10:57:10Z","timestamp":1524913030000},"page":"429-435","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Association of tumor necrosis factor alpha gene polymorphisms with <i>Helicobacter pylori<\/i> infection in dyspeptic patients in Sri Lanka"],"prefix":"10.1111","volume":"62","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-4622-3117","authenticated-orcid":false,"given":"Piyumali Sandareka","family":"Arachchi","sequence":"first","affiliation":[{"name":"Department of Microbiology Faculty of Medical Sciences University of Sri Jayewardenepura Gangodawila Nugegoda Sri Lanka"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Manjula Manoji","family":"Weerasekera","sequence":"additional","affiliation":[{"name":"Department of Microbiology Faculty of Medical Sciences University of Sri Jayewardenepura Gangodawila Nugegoda Sri Lanka"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bimalka","family":"Senevirathna","sequence":"additional","affiliation":[{"name":"Department of Pathology Faculty of Medical Sciences University of Sri Jayewardenepura Gangodawila Nugegoda Sri Lanka"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Deepaka","family":"Weerasekera","sequence":"additional","affiliation":[{"name":"Department of Surgery Faculty of Medical Sciences University of Sri Jayewardenepura Gangodawila Nugegoda Sri Lanka"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Neluka","family":"Fernando","sequence":"additional","affiliation":[{"name":"Department of Microbiology Faculty of Medical Sciences University of Sri Jayewardenepura Gangodawila Nugegoda Sri Lanka"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chinthika Prabhashinie","family":"Gunasekara","sequence":"additional","affiliation":[{"name":"Department of Microbiology Faculty of Medical Sciences University of Sri Jayewardenepura Gangodawila Nugegoda Sri Lanka"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2018,6,12]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"crossref","first-page":"2346","DOI":"10.1128\/IAI.66.5.2346-2348.1998","article-title":"Proteins encoded by the cag pathogenicity island of Helicobacter pylori are required for NF\u2010\u03baB activation","volume":"66","author":"Glocker E.","year":"1998","journal-title":"Infect Immun"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1136\/gut.38.4.498"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1080\/003655200750024515"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2036.2000.014s1188.x"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1093\/jnci\/89.12.863"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-3083.2011.02602.x"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/13.17.6361"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1093\/hmg\/ddg262"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2249.1998.00662.x"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0198-8859(01)00322-6"},{"key":"e_1_2_8_12_1","first-page":"47","article-title":"TNF alpha promoter polymorphisms","volume":"98","author":"Fargion S.","year":"2004","journal-title":"Methods Mol Med"},{"key":"e_1_2_8_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cyto.2014.07.005"},{"key":"e_1_2_8_14_1","doi-asserted-by":"publisher","DOI":"10.1158\/1055-9965.EPI-06-0267"},{"key":"e_1_2_8_15_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10038-008-0273-3"},{"key":"e_1_2_8_16_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.bjc.6604277"},{"key":"e_1_2_8_17_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1572-0241.2005.40806.x"},{"key":"e_1_2_8_18_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1523-5378.2006.00384.x"},{"key":"e_1_2_8_19_1","first-page":"51","article-title":"Different effects of polymorphisms of tumor necrosis factor\u2010 alpha and interleukin\u20101 beta on development of peptic ulcer and gastric cancer","volume":"22","author":"Sugimoto M.","year":"2007","journal-title":"Gastroenterology"},{"key":"e_1_2_8_20_1","doi-asserted-by":"publisher","DOI":"10.4103\/1319-3767.114513"},{"key":"e_1_2_8_21_1","doi-asserted-by":"publisher","DOI":"10.4038\/cmj.v60i4.8224"},{"key":"e_1_2_8_22_1","doi-asserted-by":"publisher","DOI":"10.17795\/ijep29796"},{"key":"e_1_2_8_23_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1440-1746.1991.tb01468.x"},{"key":"e_1_2_8_24_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.clinre.2016.05.014"},{"key":"e_1_2_8_25_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0304-3835(01)00438-4"},{"key":"e_1_2_8_26_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-3156.2010.02491.x"},{"key":"e_1_2_8_27_1","doi-asserted-by":"publisher","DOI":"10.4103\/0974-777X.170501"},{"key":"e_1_2_8_28_1","doi-asserted-by":"publisher","DOI":"10.1007\/s13277-013-0972-z"},{"key":"e_1_2_8_29_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1572-0241.2005.40852.x"}],"container-title":["Microbiology and Immunology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2F1348-0421.12597","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/1348-0421.12597","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,18]],"date-time":"2023-09-18T02:19:17Z","timestamp":1695003557000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/1348-0421.12597"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2018,6,12]]},"references-count":28,"journal-issue":{"issue":"7","published-print":{"date-parts":[[2018,7]]}},"alternative-id":["10.1111\/1348-0421.12597"],"URL":"https:\/\/doi.org\/10.1111\/1348-0421.12597","archive":["Portico"],"relation":{},"ISSN":["0385-5600","1348-0421"],"issn-type":[{"type":"print","value":"0385-5600"},{"type":"electronic","value":"1348-0421"}],"subject":[],"published":{"date-parts":[[2018,6,12]]},"assertion":[{"value":"2018-01-23","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2018-04-26","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2018-06-12","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}