{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2024,8,14]],"date-time":"2024-08-14T21:53:52Z","timestamp":1723672432144},"reference-count":38,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2018,7,16]],"date-time":"2018-07-16T00:00:00Z","timestamp":1531699200000},"content-version":"vor","delay-in-days":15,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Microbiology and Immunology"],"published-print":{"date-parts":[[2018,7]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:sec><jats:label \/><jats:p>In this study, 672 plant\u2010tissue extracts were screened for phytochemicals that inhibit the function of the type III secretion system (T3SS) of enteropathogenic <jats:italic>Escherichia coli<\/jats:italic> (EPEC) and enterohemorrhagic <jats:italic>E. coli<\/jats:italic> (EHEC). Among candidates examined, an extract from the leaves of <jats:italic>Psidium guajava<\/jats:italic> (guava) was found to inhibit secretion of EPEC\u2010secreted protein B (EspB) from EPEC and EHEC without affecting bacterial growth. Guava extract (GE) also inhibited EPEC and EHEC from adhering to, and injecting EspB into, HEp\u20102 cells. GE seemed to block translocation of EspB from the bacterial cells to the culture medium. In addition, GE also inhibited the T3SS of <jats:italic>Yersinia pseudotuberculosis<\/jats:italic> and <jats:italic>Salmonella enterica<\/jats:italic> serovar Typhimurium. After exposure to GE, <jats:italic>Y. pseudotuberculosis<\/jats:italic> stopped secreting <jats:italic>Yersinia<\/jats:italic> outer proteins and was unable to induce apoptosis of mouse bone marrow\u2010derived macrophages. <jats:italic>S. typhimurium<\/jats:italic> exposed to GE stopped secreting Sip proteins and was unable to invade HEp\u20102 cells. GE inhibited secretion of EspC, the type V secretion protein of EPEC, but not secretion of Shiga toxin 2 from EHEC. Thus, our results suggest that guava leaves contain a novel type of antimicrobial compound that could be used to treat and prevent gram\u2010negative enteropathogenic bacterial infections.<\/jats:p><\/jats:sec>","DOI":"10.1111\/1348-0421.12604","type":"journal-article","created":{"date-parts":[[2018,5,23]],"date-time":"2018-05-23T11:41:29Z","timestamp":1527075689000},"page":"444-453","update-policy":"http:\/\/dx.doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["Effects of <i>Psidium guajava<\/i> leaf extract on secretion systems of gram\u2010negative enteropathogenic bacteria"],"prefix":"10.1111","volume":"62","author":[{"given":"Noboru","family":"Nakasone","sequence":"first","affiliation":[{"name":"Department of Bacteriology Graduate School of Medicine, University of the Ryukyus Nishihara Okinawa 903\u20100213 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