{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2024,4,5]],"date-time":"2024-04-05T07:16:01Z","timestamp":1712301361860},"reference-count":19,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2018,7,25]],"date-time":"2018-07-25T00:00:00Z","timestamp":1532476800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Microbiology and Immunology"],"published-print":{"date-parts":[[2018,8]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:sec><jats:label \/><jats:p>JC polyomavirus (JCPyV) is the causative agent of progressive multifocal leukoencephalopathy (PML), a demyelinating disease of the central nervous system in immunocompromised patients. Archetype JCPyV circulates in the human population. There have been several reports of archetype JCPyV replication in cultured cells, in which propagation was not enough to produce high titers of archetype JCPyV. In this study, we carried out cultivation of the transfected cells with archetype JCPyV DNA MY for more than 2 months to establish COS\u20107 cells (designated COS\u2010JC cells) persistently producing archetype JCPyV. Moreover, JCPyV derived from COS\u2010JC cells was characterized by analyzing the viral propagation, size of the viral genome, amount of viral DNA, production of viral protein, and structure of the non\u2010coding control region (NCCR). Southern blotting using a digoxigenin\u2010labeled JCPyV probe showed two different sizes of the JCPyV genome in COS\u2010JC cells. For molecular cloning, four of five clones showed a decrease in the size of complete JCPyV genome. Especially, clone No. 10 was generated the large deletion within the Large T antigen. On the other hand, the archetype structure of the NCCR was maintained in COS\u2010JC cells, although a few point mutations occurred. Quantitative PCR analysis of viral DNA in COS\u2010JC cells indicated that a high copy number of archetype JCPyV DNA was replicated in COS\u2010JC cells. These findings suggest that COS\u2010JC cells could efficiently propagate archetype JCPyV MY and offer a useful tool to study persistent infection of archetype JCPyV in a kidney\u2010derived system.<\/jats:p><\/jats:sec>","DOI":"10.1111\/1348-0421.12632","type":"journal-article","created":{"date-parts":[[2018,6,22]],"date-time":"2018-06-22T11:58:06Z","timestamp":1529668686000},"page":"524-530","update-policy":"http:\/\/dx.doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Establishment of COS\u2010JC cells persistently producing archetype JC polyomavirus"],"prefix":"10.1111","volume":"62","author":[{"given":"Souichi","family":"Nukuzuma","sequence":"first","affiliation":[{"name":"Department of Infectious Diseases Kobe Institute of Health 4\u20106\u20105, Minatojima \u2010Nakamachi Chuo\u2010ku, Kobe 650\u20100046 Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chiyoko","family":"Nukuzuma","sequence":"additional","affiliation":[{"name":"Thermocell Inc. Shinagawa\u2010ku Tokyo 141\u20100022 Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Masanori","family":"Kameoka","sequence":"additional","affiliation":[{"name":"Department of International Health Kobe University Graduate School of Health Sciences Suma\u2010ku Kobe 615\u20100124 Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shigeki","family":"Sugiura","sequence":"additional","affiliation":[{"name":"Medical Genetics Research Center Nara Medical University Kashihara Nara 634\u20108521 Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kazuo","family":"Nakamichi","sequence":"additional","affiliation":[{"name":"Department of Virology 1 National Institute of Infectious Diseases Toyama, Shinjuku Tokyo 162\u20108640 Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Takafumi","family":"Tasaki","sequence":"additional","affiliation":[{"name":"Divison of Protein Regulation Research Medical Research Institute Kanazawa Medical University Ishikawa 920\u20100293 Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Koushi","family":"Hidaka","sequence":"additional","affiliation":[{"name":"Faculty of Pharmaceutical Sciences Kobe 650\u20108586 Japan"},{"name":"Cooperative Research Center for Life Sciences Kobe Gakuin University Kobe 650\u20108586 Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tsutomu","family":"Takegami","sequence":"additional","affiliation":[{"name":"Division of Molecular Oncology and Virology Medical Research Institute Kanazawa Medical University Ishikawa 920\u20100293 Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2018,7,25]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(71)91777-6"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/0042-6822(92)90040-V"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.5.1.49"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.51.2.458-469.1984"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.64.6.3139-3143.1990"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.05031-11"},{"key":"e_1_2_8_8_1","doi-asserted-by":"crossref","first-page":"5335","DOI":"10.1128\/JVI.72.7.5335-5342.1998","article-title":"Archetype JC virus efficiently replicates in COS\u20107 cells, simian cells constitutively expressing simian virus 40 T antigen","volume":"72","author":"Hara K.","year":"1998","journal-title":"J Virol"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00705-017-3542-7"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1348-0421.2009.00166.x"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.virol.2011.10.026"},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.1002\/jmv.23239"},{"key":"e_1_2_8_13_1","doi-asserted-by":"publisher","DOI":"10.1002\/jmv.1890470413"},{"key":"e_1_2_8_14_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1348-0421.2009.00156.x"},{"key":"e_1_2_8_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/0022-2836(67)90307-5"},{"key":"e_1_2_8_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jcv.2004.12.018"},{"key":"e_1_2_8_17_1","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.03714-14"},{"key":"e_1_2_8_18_1","doi-asserted-by":"publisher","DOI":"10.1099\/0022-1317-74-8-1499"},{"key":"e_1_2_8_19_1","doi-asserted-by":"publisher","DOI":"10.1099\/0022-1317-64-10-2271"},{"key":"e_1_2_8_20_1","doi-asserted-by":"crossref","first-page":"306","DOI":"10.1128\/jvi.53.1.306-311.1985","article-title":"Differences in regulatory sequences of naturally occurring JC virus variants","volume":"53","author":"Martin J.D.","year":"1985","journal-title":"J Virol"}],"container-title":["Microbiology and Immunology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2F1348-0421.12632","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/1348-0421.12632","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,18]],"date-time":"2023-09-18T01:00:39Z","timestamp":1694998839000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/1348-0421.12632"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2018,7,25]]},"references-count":19,"journal-issue":{"issue":"8","published-print":{"date-parts":[[2018,8]]}},"alternative-id":["10.1111\/1348-0421.12632"],"URL":"https:\/\/doi.org\/10.1111\/1348-0421.12632","archive":["Portico"],"relation":{},"ISSN":["0385-5600","1348-0421"],"issn-type":[{"value":"0385-5600","type":"print"},{"value":"1348-0421","type":"electronic"}],"subject":[],"published":{"date-parts":[[2018,7,25]]},"assertion":[{"value":"2018-02-11","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2018-06-13","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2018-07-25","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}