{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,9,26]],"date-time":"2025-09-26T04:58:34Z","timestamp":1758862714427},"reference-count":54,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2018,10,21]],"date-time":"2018-10-21T00:00:00Z","timestamp":1540080000000},"content-version":"vor","delay-in-days":20,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"Ministry of Higher Education (LRGS\/TD\/2011\/UM\/Penyakit Berjangkit)"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Microbiology and Immunology"],"published-print":{"date-parts":[[2018,10]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:sec><jats:label \/><jats:p>Dengue is the most prevalent mosquito\u2010borne disease in Southeast Asia, where the incidence of systemic lupus erythematosus (SLE) is approximately 30 to 53 per 100,000. Severe dengue, however, is rarely reported among individuals with SLE. Here, whether sera of patients with SLE cross\u2010neutralize dengue virus (DENV) was investigated. Serum samples were obtained from individuals with SLE who were dengue IgG and IgM serology negative. Neutralization assays were performed against the three major DENV serotypes. Of the dengue serology negative sera of individuals with SLE, 60%, 61% and 52% of the sera at 1\/320 dilution showed more than 50% inhibition against dengue type\u20101 virus (DENV\u20101), DENV\u20102 and DENV\u20103, respectively. The neutralizing capacity of the sera was significantly greater against DENV\u20101 (<jats:italic>P<\/jats:italic>\u2009&lt;\u20090.001) and DENV\u20103 (<jats:italic>P<\/jats:italic>\u2009&lt;\u20090.01) than against DENV\u20102 (<jats:italic>P<\/jats:italic>\u2009&lt;\u20090.05). Neutralization against the DENV correlated with dengue\u2010specific IgG serum titers below the cut\u2010off point for dengue positivity. Depletion of total IgG from the sera of patients with SLE resulted in significant decreases of up to 80% in DENV inhibition, suggesting that IgG plays an important role. However, some of the SLE sera was still able to neutralize DENV, even with IgG titers &lt;0.1 OD absorbance. Our findings suggest that sera of patients with SLE contain IgG, and possibly other type of antibodies, that can cross\u2010neutralize DENV, which may explain the rarity of severe dengue in individuals with SLE. Further studies, are needed to further substantiate this finding and to elucidate the specific neutralizing epitopes recognized by the sera of individuals with SLE.<\/jats:p><\/jats:sec>","DOI":"10.1111\/1348-0421.12652","type":"journal-article","created":{"date-parts":[[2018,9,27]],"date-time":"2018-09-27T08:28:51Z","timestamp":1538036931000},"page":"659-672","update-policy":"http:\/\/dx.doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["Sera of patients with systemic lupus erythematosus cross\u2010neutralizes dengue viruses"],"prefix":"10.1111","volume":"62","author":[{"given":"Nurhafiza","family":"Zainal","sequence":"first","affiliation":[{"name":"Institute of Graduate Studies University of Malaya Kuala Lumpur Malaysia"},{"name":"Department of Medical Microbiology, Faculty of Medicine University of Malaya Kuala Lumpur Malaysia"},{"name":"Institute of Basic Medical Sciences College of Medicine, National Cheng Kung University Tainan Taiwan"},{"name":"Department of Microbiology and Immunology College of Medicine, National Cheng Kung University Tainan Taiwan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kim\u2010Kee","family":"Tan","sequence":"additional","affiliation":[{"name":"Department of Medical Microbiology, Faculty of Medicine University of Malaya Kuala Lumpur Malaysia"},{"name":"Tropical Infectious Diseases Research and Education Centre (TIDREC) University of Malaya Kuala Lumpur Malaysia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jefree","family":"Johari","sequence":"additional","affiliation":[{"name":"Tropical Infectious Diseases Research and Education Centre (TIDREC) University of Malaya Kuala Lumpur Malaysia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Heselynn","family":"Hussein","sequence":"additional","affiliation":[{"name":"Subang Jaya Medical Centre Subang Jaya Selangor"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wan Rosmaiza","family":"Wan Musa","sequence":"additional","affiliation":[{"name":"Department of Rheumatology Putrajaya Hospital Putrajaya"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jamiyah","family":"Hassan","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynaecology, Faculty of Medicine University of Malaya Kuala Lumpur Malaysia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yee\u2010Shin","family":"Lin","sequence":"additional","affiliation":[{"name":"Institute of Basic Medical Sciences College of Medicine, National Cheng Kung University Tainan Taiwan"},{"name":"Department of Microbiology and Immunology College of Medicine, National Cheng Kung University Tainan Taiwan"},{"name":"Centre of Infectious Disease and Signalling Research National Cheng Kung University Tainan Taiwan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sazaly","family":"AbuBakar","sequence":"additional","affiliation":[{"name":"Institute of Graduate Studies University of Malaya Kuala Lumpur Malaysia"},{"name":"Department of Medical Microbiology, Faculty of Medicine University of Malaya Kuala Lumpur Malaysia"},{"name":"Tropical Infectious Diseases Research and Education Centre (TIDREC) University of Malaya Kuala Lumpur Malaysia"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2018,10,21]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1258\/ebm.2011.010339"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1089\/vim.2006.19.127"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jfma.2012.11.006"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0029430"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1186\/1743-422X-7-28"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pntd.0001188"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.4269\/ajtmh.1952.1.30"},{"key":"e_1_2_8_9_1","first-page":"350","article-title":"Observations related to pathogenesis of dengue hemorrhagic fever. VI. Hypotheses and discussion","volume":"42","author":"Halstead S.","year":"1970","journal-title":"Yale J Biol Med"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.4269\/ajtmh.1988.38.172"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.7150\/ijms.8988"},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1607931113"},{"key":"e_1_2_8_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.vaccine.2016.10.018"},{"key":"e_1_2_8_14_1","doi-asserted-by":"publisher","DOI":"10.1172\/JCI73441"},{"key":"e_1_2_8_15_1","doi-asserted-by":"publisher","DOI":"10.1053\/jinf.2001.0959"},{"key":"e_1_2_8_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0022-1759(02)00236-3"},{"key":"e_1_2_8_17_1","doi-asserted-by":"publisher","DOI":"10.1084\/jem.20091281"},{"key":"e_1_2_8_18_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMra1100359"},{"key":"e_1_2_8_19_1","doi-asserted-by":"crossref","first-page":"0161818","DOI":"10.1371\/journal.pone.0161818","article-title":"The use of poly\u2010L\u2010lysine as a capture agent to enhance the detection of antinuclear antibodies by ELISA","volume":"11","author":"Stearns N.A.","year":"2016","journal-title":"PLoS ONE"},{"key":"e_1_2_8_20_1","doi-asserted-by":"publisher","DOI":"10.1177\/0961203312437807"},{"key":"e_1_2_8_21_1","doi-asserted-by":"publisher","DOI":"10.4269\/ajtmh.15-0326"},{"key":"e_1_2_8_22_1","doi-asserted-by":"publisher","DOI":"10.1002\/art.1780251101"},{"key":"e_1_2_8_23_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12016-009-8192-4"},{"key":"e_1_2_8_24_1","doi-asserted-by":"publisher","DOI":"10.1089\/vim.2006.0100"},{"key":"e_1_2_8_25_1","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2148-13-213"},{"key":"e_1_2_8_26_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.meegid.2017.07.008"},{"key":"e_1_2_8_27_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pntd.0002274"},{"key":"e_1_2_8_28_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-3156.2007.01967.x"},{"key":"e_1_2_8_29_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jim.2014.03.013"},{"key":"e_1_2_8_30_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.rbr.2013.02.003"},{"key":"e_1_2_8_31_1","doi-asserted-by":"publisher","DOI":"10.2147\/IMCRJ.S50708"},{"key":"e_1_2_8_32_1","first-page":"147","article-title":"Dengue viral infection triggering abnormal immune response in a case of Kikuchi disease which later evolved into SLE","volume":"64","author":"Sane P.","year":"2016","journal-title":"J Assoc Phys India"},{"key":"e_1_2_8_33_1","doi-asserted-by":"publisher","DOI":"10.7150\/ijms.13680"},{"key":"e_1_2_8_34_1","doi-asserted-by":"publisher","DOI":"10.1002\/art.1780400623"},{"key":"e_1_2_8_35_1","first-page":"2711","article-title":"Emergence of systemic lupus erythematosus after initiation of highly active antiretroviral therapy for human immunodeficiency virus infection","volume":"27","author":"Diri E.","year":"2000","journal-title":"J Rheumatol"},{"key":"e_1_2_8_36_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0272-6386(99)70180-0"},{"key":"e_1_2_8_37_1","doi-asserted-by":"publisher","DOI":"10.1172\/JCI73441"},{"key":"e_1_2_8_38_1","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.01023-12"},{"key":"e_1_2_8_39_1","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.00326-11"},{"key":"e_1_2_8_40_1","doi-asserted-by":"publisher","DOI":"10.1093\/infdis\/161.2.198"},{"key":"e_1_2_8_41_1","doi-asserted-by":"publisher","DOI":"10.1136\/ard.49.6.387"},{"key":"e_1_2_8_42_1","doi-asserted-by":"publisher","DOI":"10.3181\/0805-RM-147"},{"key":"e_1_2_8_43_1","doi-asserted-by":"publisher","DOI":"10.4269\/ajtmh.14-0162"},{"key":"e_1_2_8_44_1","doi-asserted-by":"publisher","DOI":"10.1038\/ncprheum0895"},{"key":"e_1_2_8_45_1","doi-asserted-by":"publisher","DOI":"10.1099\/0022-1317-73-8-1995"},{"key":"e_1_2_8_46_1","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.05541-11"},{"key":"e_1_2_8_47_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00430-016-0457-y"},{"key":"e_1_2_8_48_1","doi-asserted-by":"publisher","DOI":"10.1002\/pmic.200401331"},{"key":"e_1_2_8_49_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11882-011-0216-5"},{"key":"e_1_2_8_50_1","doi-asserted-by":"publisher","DOI":"10.1038\/ni.3326"},{"key":"e_1_2_8_51_1","first-page":"41","article-title":"Antibody responses of dengue fever patients to dengue 2 (New Guinea C strain) viral proteins","volume":"19","author":"AbuBakar S.","year":"1997","journal-title":"Malays J Pathol"},{"key":"e_1_2_8_52_1","doi-asserted-by":"publisher","DOI":"10.1089\/088922202753519142"},{"key":"e_1_2_8_53_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0091-6749(99)70087-4"},{"key":"e_1_2_8_54_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.humimm.2005.07.009"},{"key":"e_1_2_8_55_1","doi-asserted-by":"publisher","DOI":"10.1099\/0022-1317-67-12-2663"}],"container-title":["Microbiology and Immunology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2F1348-0421.12652","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/1348-0421.12652","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,16]],"date-time":"2023-09-16T17:13:20Z","timestamp":1694884400000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/1348-0421.12652"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2018,10]]},"references-count":54,"journal-issue":{"issue":"10","published-print":{"date-parts":[[2018,10]]}},"alternative-id":["10.1111\/1348-0421.12652"],"URL":"https:\/\/doi.org\/10.1111\/1348-0421.12652","archive":["Portico"],"relation":{},"ISSN":["0385-5600","1348-0421"],"issn-type":[{"value":"0385-5600","type":"print"},{"value":"1348-0421","type":"electronic"}],"subject":[],"published":{"date-parts":[[2018,10]]},"assertion":[{"value":"2018-03-30","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2018-09-10","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2018-10-21","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}