{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,28]],"date-time":"2026-09-28T17:40:45Z","timestamp":1790617245470,"version":"4.1.0"},"reference-count":35,"publisher":"Wiley","issue":"11","license":[{"start":{"date-parts":[[2018,11,21]],"date-time":"2018-11-21T00:00:00Z","timestamp":1542758400000},"content-version":"vor","delay-in-days":20,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"Department of Biotechnology, Government of India","award":["DBT-NER\/LIVS\/11\/2012"],"award-info":[{"award-number":["DBT-NER\/LIVS\/11\/2012"]}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Microbiology and Immunology"],"published-print":{"date-parts":[[2018,11]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:sec><jats:label\/><jats:p>Brucellosis caused by the bacteria of the genus <jats:italic>Brucella<\/jats:italic> is an important zoonosis and constitutes a serious public health hazard. Brucellosis is diagnosed mainly by the Rose Bengal plate test and indirect ELISA, both of which have poor specificity because false positive serological reactions occur when screening animals that have been vaccinated with <jats:italic>B. abortus<\/jats:italic> S19. Fluorescence polarization assay (FPA) was evaluated for screening samples from cattle and buffalo calves with persistent antibody titers induced by vaccination. The standardized FPA exhibited relative sensitivity and specificity of 0.94 and 0.95, respectively, and the area under the curve, kappa and accuracy were 0.98, 0.87 and 0.95, respectively. Comparison of competitive ELISA and FPA revealed that, FPA is more specific than competitive ELISA. The high specificity, sensitivity and 95% accuracy of FPA indicate that, it is suitable for testing vaccinated animals because it can distinguish between infected from vaccinated animals.<\/jats:p><\/jats:sec>","DOI":"10.1111\/1348-0421.12654","type":"journal-article","created":{"date-parts":[[2018,10,23]],"date-time":"2018-10-23T06:00:05Z","timestamp":1540274405000},"page":"694-701","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["Assessment of fluorescence polarization assay: a candid diagnostic tool in <i>Brucella abortus<\/i> strain 19 vaccinated areas"],"prefix":"10.1111","volume":"62","author":[{"given":"Triveni","family":"Kalleshamurthy","sequence":"first","affiliation":[{"name":"Indian Council Agricultural Research\u2013National Institute of Veterinary Epidemiology and Disease Informatics Ramagondanahalli, Yelahanka Bengaluru 560064 India"},{"name":"Centre for Post Graduate Studies Jain University Jaya Nagar, 3 block Bengaluru 560041 India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ranjitha","family":"Shekar","sequence":"additional","affiliation":[{"name":"Indian Council Agricultural Research\u2013National Institute of Veterinary Epidemiology and Disease Informatics Ramagondanahalli, Yelahanka Bengaluru 560064 India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Harish Heballi","family":"Niranjanamurthy","sequence":"additional","affiliation":[{"name":"Indian Council Agricultural Research\u2013National Institute of Veterinary Epidemiology and Disease Informatics Ramagondanahalli, Yelahanka Bengaluru 560064 India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Krithiga","family":"Natesan","sequence":"additional","affiliation":[{"name":"Indian Council Agricultural Research\u2013National Institute of Veterinary Epidemiology and Disease Informatics Ramagondanahalli, Yelahanka Bengaluru 560064 India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bibek Ranjan","family":"Shome","sequence":"additional","affiliation":[{"name":"Indian Council Agricultural Research\u2013National Institute of Veterinary Epidemiology and Disease Informatics Ramagondanahalli, Yelahanka Bengaluru 560064 India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rajendra Gulabrao","family":"Bambal","sequence":"additional","affiliation":[{"name":"Department of Animal Husbandry Dairying and Fisheries Krishi Bhavan New Delhi 110001 India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lipi","family":"Sairiwal","sequence":"additional","affiliation":[{"name":"Department of Animal Husbandry Dairying and Fisheries Krishi Bhavan New Delhi 110001 India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sukhadeo B.","family":"Barbuddhe","sequence":"additional","affiliation":[{"name":"ICAR\u2010National Research Centre on Meat Chengicherla Hyderabad 500092 India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Amol","family":"Sahare","sequence":"additional","affiliation":[{"name":"Intervet India MSD Animal Health Briahnagar, Off Pune\u2010Nagar Road Wagholi 412207 Pune India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sreenivasulu","family":"Kilari","sequence":"additional","affiliation":[{"name":"Intervet India MSD Animal Health Briahnagar, Off Pune\u2010Nagar Road Wagholi 412207 Pune India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Habibur","family":"Rahman","sequence":"additional","affiliation":[{"name":"International Livestock Research Institute NASC Complex, CG Centre, Pusa New Delhi 110012 India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rajeswari","family":"Shome","sequence":"additional","affiliation":[{"name":"Indian Council Agricultural Research\u2013National Institute of Veterinary Epidemiology and Disease Informatics Ramagondanahalli, Yelahanka Bengaluru 560064 India"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2018,11,21]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.prevetmed.2011.04.007"},{"key":"e_1_2_8_3_1","doi-asserted-by":"crossref","first-page":"2090","DOI":"10.1099\/ijsem.0.000998","article-title":"Brucella vulpis sp. nov., isolated from mandibular lymph nodes of red foxes (Vulpes vulpes)","volume":"66","author":"Scholz H.C.","year":"2016","journal-title":"Int J Syst Evol Microbiol"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1017\/S0950268807008795"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1086\/501359"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12038-008-0072-1"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.vetmic.2009.06.021"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.3325\/cmj.2010.51.306"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12941-014-0031-7"},{"key":"e_1_2_8_10_1","unstructured":"OIE: World Organization for Animal Health. (2016) Brucellosis (Brucella abortus B. melitensisandB. suis) (Infection withB. abortus B. melitensisandB.suis). Chapter 2.1.4."},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11250-008-9244-0"},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.41.1.144-148.2003"},{"key":"e_1_2_8_13_1","doi-asserted-by":"publisher","DOI":"10.20506\/rst.23.3.1545"},{"key":"e_1_2_8_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.vetmic.2003.12.010"},{"key":"e_1_2_8_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/0165-2427(88)90108-0"},{"key":"e_1_2_8_16_1","doi-asserted-by":"publisher","DOI":"10.1081\/IAS-120015478"},{"key":"e_1_2_8_17_1","unstructured":"Department of Animal Husbandry Dairying & Fisheries Ministry of Agriculture & Farmers Welfare Government of India. (2017) Annual report 2016\u20132017. Chapter 3.7.8. Brucellosis Control Program. [Cited 8 May 2018] Available from URL:http:\/\/dahd.nic.in\/sites\/default\/filess\/Annual%20Report%202016-17.pdf"},{"key":"e_1_2_8_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.prevetmed.2018.01.005"},{"key":"e_1_2_8_19_1","first-page":"271","article-title":"Detection of Brucella melitensis and Brucella abortus by DNA amplification","volume":"95","author":"Baily G.G.","year":"1992","journal-title":"J Trop Med Hyg"},{"key":"e_1_2_8_20_1","volume-title":"Techniques for the Brucellosis Laboratory","author":"Alton G.G.","year":"1988"},{"key":"e_1_2_8_21_1","doi-asserted-by":"crossref","first-page":"2660","DOI":"10.1128\/jcm.32.11.2660-2666.1994","article-title":"Differentiation of Brucella abortus bv 1, 2 and 4, Brucella melitensis, Brucella ovis, and Brucella suis bv 1 by PCR","volume":"32","author":"Bricker B.J.","year":"1994","journal-title":"J Clin Microbiol"},{"key":"e_1_2_8_22_1","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.00837-08"},{"key":"e_1_2_8_23_1","unstructured":"US Department of Agriculture Animal and Plant Health Inspection Service. Fluorescence polarization assay test. Field trial and testing results for validation of the FP test in cattle bison and swine. [Cited 24 May 2018] Available from URL:https:\/\/www.aphis.usda.gov\/animal_health\/animal_diseases\/brucellosis\/downloads\/fpa-val-rpt.pdf"},{"key":"e_1_2_8_24_1","doi-asserted-by":"publisher","DOI":"10.11613\/BM.2012.031"},{"key":"e_1_2_8_25_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.prevetmed.2015.03.013"},{"key":"e_1_2_8_26_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0378-1135(02)00229-8"},{"key":"e_1_2_8_27_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11250-011-9831-3"},{"key":"e_1_2_8_28_1","doi-asserted-by":"publisher","DOI":"10.3325\/cmj.2010.51.296"},{"key":"e_1_2_8_29_1","doi-asserted-by":"publisher","DOI":"10.20506\/rst.24.3.1628"},{"key":"e_1_2_8_30_1","article-title":"Dynamics of antibody response in buffalo calves vaccinated with Brucella abortus S19 using various serological assays","volume":"12","author":"Chand P.","year":"2010","journal-title":"J Immunol Immunopathol"},{"key":"e_1_2_8_31_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.vetimm.2016.02.002"},{"key":"e_1_2_8_32_1","doi-asserted-by":"publisher","DOI":"10.1016\/0022-1759(96)00116-0"},{"key":"e_1_2_8_33_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11250-011-9794-4"},{"key":"e_1_2_8_34_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.vetmic.2007.05.012"},{"key":"e_1_2_8_35_1","doi-asserted-by":"publisher","DOI":"10.1099\/jmm.0.05217-0"},{"key":"e_1_2_8_36_1","doi-asserted-by":"publisher","DOI":"10.4172\/2325\u20109590.1000140"}],"container-title":["Microbiology and Immunology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2F1348-0421.12654","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/1348-0421.12654","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,15]],"date-time":"2023-09-15T17:22:25Z","timestamp":1694798545000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/1348-0421.12654"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2018,11]]},"references-count":35,"journal-issue":{"issue":"11","published-print":{"date-parts":[[2018,11]]}},"alternative-id":["10.1111\/1348-0421.12654"],"URL":"https:\/\/doi.org\/10.1111\/1348-0421.12654","archive":["Portico"],"relation":{},"ISSN":["0385-5600","1348-0421"],"issn-type":[{"value":"0385-5600","type":"print"},{"value":"1348-0421","type":"electronic"}],"subject":[],"published":{"date-parts":[[2018,11]]},"assertion":[{"value":"2018-07-21","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2018-10-14","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2018-11-21","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}