{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,24]],"date-time":"2026-09-24T00:45:10Z","timestamp":1790210710367,"version":"4.1.0"},"reference-count":50,"publisher":"Wiley","issue":"12","license":[{"start":{"date-parts":[[2018,12,27]],"date-time":"2018-12-27T00:00:00Z","timestamp":1545868800000},"content-version":"vor","delay-in-days":26,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001691","name":"Japan Society for the Promotion of Science","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100001691","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Microbiology and Immunology"],"published-print":{"date-parts":[[2018,12]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:sec><jats:label\/><jats:p>An effective vaccine against <jats:italic>Pseudomonas aeruginosa<\/jats:italic> would be hugely beneficial to people who are susceptible to the serious infections it can cause. Vaccination against PcrV of the <jats:italic>P. aeruginosa<\/jats:italic> type III secretion system is a potential prophylactic strategy for improving the incidence and prognosis of <jats:italic>P. aeruginosa<\/jats:italic> pneumonia. Here, the effect of nasal PcrV adjuvanted with CpG oligodeoxynucleotide (CpG) was compared with a nasal PcrV\/aluminum hydroxide gel (alum) vaccine. Seven groups of mice were vaccinated intranasally with one of the following: 1, PcrV\u2010CpG; 2, PcrV\u2010alum; 3, PcrV alone; 4, CpG alone; 5, alum alone; 6 and 7, saline control. Fifty days after the first immunization, anti\u2010PcrV IgG, IgA and IgG isotype titers were measured; significant increases in these titers were detected only in the PcrV\u2010CpG vaccinated mice. The vaccinated mice were then intratracheally infected with a lethal dose of <jats:italic>P. aeruginosa<\/jats:italic> and their body temperatures and survival monitored for 24\u2009hr, edema, bacteria, myeloperoxidase activity and lung histology also being evaluated at 24\u2009hr post\u2010infection. It was found that 73% of the PcrV\u2010CpG\u2010vaccinated mice survived, whereas fewer than 30% of the mice vaccinated with PcrV\u2010alum or adjuvant alone survived. Lung edema and other inflammation\u2010related variables were less severe in the PcrV\u2010CpG group. The significant increase in PcrV\u2010specific IgA titers detected following PcrV\u2010CpG vaccination is probably a component of the disease protection mechanism. Overall, our data show that intranasal PcrV\u2010CpG vaccination has potential efficacy for clinical application against <jats:italic>P. aeruginosa<\/jats:italic> pneumonia.<\/jats:p><\/jats:sec>","DOI":"10.1111\/1348-0421.12658","type":"journal-article","created":{"date-parts":[[2018,10,31]],"date-time":"2018-10-31T10:55:31Z","timestamp":1540983331000},"page":"774-785","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":31,"title":["The protective effects of nasal PcrV\u2010CpG oligonucleotide vaccination against <i>Pseudomonas aeruginosa<\/i> pneumonia"],"prefix":"10.1111","volume":"62","author":[{"given":"Yoshifumi","family":"Naito","sequence":"first","affiliation":[{"name":"Department of Anesthesiology Kyoto Prefectural University of Medicine 465 Kajiicho, Kawaramachi Hirokoji Kamigyo Kyoto 602\u20108566 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