{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,28]],"date-time":"2026-10-28T22:44:57Z","timestamp":1793227497517,"version":"4.5.0"},"reference-count":34,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2019,1,30]],"date-time":"2019-01-30T00:00:00Z","timestamp":1548806400000},"content-version":"vor","delay-in-days":29,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Microbiology and Immunology"],"published-print":{"date-parts":[[2019,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:label\/><jats:p>Taxonomic studies of strain PAGU 1678<jats:sup>T<\/jats:sup>, an obligately anaerobic, gram\u2010positive, spore\u2010forming bacterium isolated from biobreeding rat feces, were performed. This strain has been demonstrated to have the ability to exacerbate pathosis in a mouse model of dextran sulfate sodium\u2010induced ulcerative colitis. Phylogenetic analysis based on the 16S rRNA gene showed high homology with <jats:italic>Paraclostridium bifermentans<\/jats:italic>. To clarify the correct taxonomic position of strain PAGU 1678<jats:sup>T<\/jats:sup>, a comparative taxonomic study using <jats:italic>P. bifermentans<\/jats:italic> PAGU 2008<jats:sup>T<\/jats:sup> (\u2550JCM 1386<jats:sup>T<\/jats:sup>) and the closely related bacterial species <jats:italic>P. benzoelyticum<\/jats:italic> PAGU 2068<jats:sup>T<\/jats:sup> (\u2550LMG 28745<jats:sup>T<\/jats:sup>) was carried out. Despite the close similarity of 16S rRNA gene sequences, DNA\u2010DNA hybridization between strain PAGU 1678<jats:sup>T<\/jats:sup> and <jats:italic>P. bifermentans<\/jats:italic> PAGU 2008<jats:sup>T<\/jats:sup> was 60.03% on average, average nucleotide identity was 96.17%, and it was shown to have different genomic sequences. Biochemically, strain PAGU 1678<jats:sup>T<\/jats:sup> could be differentiated from <jats:italic>P. bifermentans<\/jats:italic> PAGU 2008<jats:sup>T<\/jats:sup> by H<jats:sub>2<\/jats:sub>S production. Furthermore, strain PAGU 1678<jats:sup>T<\/jats:sup> was characterized by the presence of two phospholipids with different polarity on polar lipid analysis. In addition, strain PAGU 1678<jats:sup>T<\/jats:sup> differed from <jats:italic>P. bifermentans<\/jats:italic> PAGU 2008<jats:sup>T<\/jats:sup> in findings on whole\u2010cell protein analysis and matrix\u2010assisted laser desorption\/ionization\u2010time of flight mass spectrometry. On the basis of these biochemical and genetic characteristics, a novel subspecies of <jats:italic>P. bifermentans<\/jats:italic> with the name <jats:italic>Paraclostridium bifermentans<\/jats:italic> subsp. <jats:italic>muricolitidis<\/jats:italic> subsp. nov. is here proposed, with PAGU 1678<jats:sup>T<\/jats:sup> (\u2550CCUG 72489<jats:sup>T<\/jats:sup> \u2550NBRC 113386<jats:sup>T<\/jats:sup>) as the type strain, which automatically creates <jats:italic>P. bifermentans<\/jats:italic> subsp. <jats:italic>bifermentans<\/jats:italic> subsp. nov. JCM 1386<jats:sup>T<\/jats:sup> (\u2550ATCC 638<jats:sup>T<\/jats:sup> \u2550DSM 14991<jats:sup>T<\/jats:sup>).<\/jats:p><\/jats:sec>","DOI":"10.1111\/1348-0421.12663","type":"journal-article","created":{"date-parts":[[2018,12,14]],"date-time":"2018-12-14T10:16:56Z","timestamp":1544782616000},"page":"1-10","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":25,"title":["Description of <i>Paraclostridium bifermentans<\/i> subsp. <i>muricolitidis<\/i> subsp. nov., emended description of <i>Paraclostridium bifermentans<\/i> (Sasi Jyothsna et al., 2016), and creation of <i>Paraclostridium bifermentans<\/i> subsp. <i>bifermentans<\/i> subsp. nov"],"prefix":"10.1111","volume":"63","author":[{"given":"Ryo","family":"Kutsuna","sequence":"first","affiliation":[{"name":"Laboratory of Microbiology Aichi Gakuin University Graduate School of Pharmaceutical Science Nagoya Aichi Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tohru","family":"Miyoshi\u2010Akiyama","sequence":"additional","affiliation":[{"name":"Pathogenic Microbe Laboratory Research Institute National Center for Global Health and Medicine Shinjuku Tokyo Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Koji","family":"Mori","sequence":"additional","affiliation":[{"name":"Biological Resource Center National Institute of Technology and Evaluation Kisarazu Chiba Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Masahiro","family":"Hayashi","sequence":"additional","affiliation":[{"name":"Division of Anaerobe Research Life Science Research Center Gifu University Gifu Japan"},{"name":"Center for Conservation of Microbial Genetic Resource Gifu University Gifu Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Junko","family":"Tomida","sequence":"additional","affiliation":[{"name":"Laboratory of Microbiology Aichi Gakuin University Graduate School of Pharmaceutical Science Nagoya Aichi Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuji","family":"Morita","sequence":"additional","affiliation":[{"name":"Laboratory of Microbiology Aichi Gakuin University Graduate School of Pharmaceutical Science Nagoya Aichi Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kaori","family":"Tanaka","sequence":"additional","affiliation":[{"name":"Division of Anaerobe Research Life Science Research Center Gifu University Gifu Japan"},{"name":"Center for Conservation of Microbial Genetic Resource Gifu University Gifu Japan"},{"name":"United Graduate School of Drug Discovery and Medical Information Sciences Gifu University Gifu Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yoshiaki","family":"Kawamura","sequence":"additional","affiliation":[{"name":"Laboratory of Microbiology Aichi Gakuin University Graduate School of Pharmaceutical Science Nagoya Aichi Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2019,1,30]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1099\/00207713-44-4-812"},{"key":"e_1_2_8_3_1","doi-asserted-by":"crossref","first-page":"1268","DOI":"10.1099\/ijsem.0.000874","article-title":"Paraclostridium benzoelyticum gen. nov., sp. nov., isolated from marine sediment and reclassification of Clostridium bifermentans as Paraclostridium bifermentans comb. nov. 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