{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,15]],"date-time":"2026-09-15T21:19:06Z","timestamp":1789507146172,"version":"build-2803163510"},"reference-count":43,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2019,6,26]],"date-time":"2019-06-26T00:00:00Z","timestamp":1561507200000},"content-version":"vor","delay-in-days":25,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100007325","name":"Kobayashi International Scholarship Foundation","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100007325","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Microbiology and Immunology"],"published-print":{"date-parts":[[2019,6]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    Hinokitiol, a component of the essential oil isolated from\n                    <jats:italic>Cupressaceae,<\/jats:italic>\n                    possesses antibacterial and antifungal activities and has been used in oral care products. In this study, the antibacterial activities of hinokitiol toward various oral, nasal and nasopharyngeal pathogenic bacteria, including\n                    <jats:italic>Streptococcus mutans<\/jats:italic>\n                    ,\n                    <jats:italic>Streptococcus sobrinus<\/jats:italic>\n                    ,\n                    <jats:italic>Porphyromonas gingivalis<\/jats:italic>\n                    ,\n                    <jats:italic>Aggregatibacter actinomycetemcomitans<\/jats:italic>\n                    ,\n                    <jats:italic>Prevotella intermedia<\/jats:italic>\n                    ,\n                    <jats:italic>Fusobacterium nucleatum<\/jats:italic>\n                    , methicillin\u2010resistant and \u2010susceptible\n                    <jats:italic>Staphylococcus aureus<\/jats:italic>\n                    , antibiotic\u2010resistant and \u2010susceptible\n                    <jats:italic>Streptococcus pneumoniae,<\/jats:italic>\n                    and\n                    <jats:italic>Streptococcus pyogenes<\/jats:italic>\n                    were examined. Growth of all these bacterial strains was significantly inhibited by hinokitiol, minimal inhibitory concentrations of hinokitiol against\n                    <jats:italic>S. mutans, S. sobrinus, P. gingivalis, P. intermedia, A. actinomycetemcomitans, F. nucleatum,<\/jats:italic>\n                    methicillin\u2010resistant\n                    <jats:italic>S. aureus,<\/jats:italic>\n                    methicillin\u2010susceptible\n                    <jats:italic>S. aureus,<\/jats:italic>\n                    antibiotic\u2010resistant\n                    <jats:italic>S. pneumoniae<\/jats:italic>\n                    isolates, antibiotic\u2010susceptible\n                    <jats:italic>S. pneumoniae,<\/jats:italic>\n                    and\n                    <jats:italic>S. pyogenes<\/jats:italic>\n                    being 0.3, 1.0, 1.0, 30, 0.5, 50, 50, 30, 0.3\u20131.0, 0.5, and 0.3\u2009\u03bcg\/mL, respectively. Additionally, with the exception of\n                    <jats:italic>P. gingivalis<\/jats:italic>\n                    , hinokitiol exerted bactericidal effects against all bacterial strains 1\u2009hr after exposure. Hinokitiol did not display any significant cytotoxicity toward the human gingival epithelial cell line Ca9\u201022, pharyngeal epithelial cell line Detroit 562, human umbilical vein endothelial cells, or human gingival fibroblasts, with the exception of treatment with 500\u2009\u03bcg\/mL hinokitiol, which decreased numbers of viable Ca9\u201022 cells and gingival fibroblasts by 13% and 12%, respectively. These results suggest that hinokitiol exhibits antibacterial activity against a broad spectrum of pathogenic bacteria and has low cytotoxicity towards human epithelial cells.\n                  <\/jats:p>","DOI":"10.1111\/1348-0421.12688","type":"journal-article","created":{"date-parts":[[2019,5,20]],"date-time":"2019-05-20T07:21:11Z","timestamp":1558336871000},"page":"213-222","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":62,"title":["Antibacterial activity of hinokitiol against both antibiotic\u2010resistant and \u2010susceptible pathogenic bacteria that predominate in the oral cavity and upper airways"],"prefix":"10.1111","volume":"63","author":[{"given":"Hisanori","family":"Domon","sequence":"first","affiliation":[{"name":"Division of Microbiology and Infectious Diseases Niigata University Graduate School of Medical and Dental Sciences Niigata Japan"},{"name":"Research Center for Advanced Oral Science Niigata University Graduate School of Medical and Dental Sciences Niigata Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Takumi","family":"Hiyoshi","sequence":"additional","affiliation":[{"name":"Division of Microbiology and Infectious Diseases Niigata University Graduate School of Medical and Dental Sciences Niigata Japan"},{"name":"Division of Periodontology Niigata University Graduate School of Medical and Dental Sciences Niigata Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tomoki","family":"Maekawa","sequence":"additional","affiliation":[{"name":"Division of Microbiology and Infectious Diseases Niigata University Graduate School of Medical and Dental Sciences Niigata Japan"},{"name":"Research Center for Advanced Oral Science Niigata University Graduate School of Medical and Dental Sciences Niigata Japan"},{"name":"Division of Periodontology Niigata University Graduate School of Medical and Dental Sciences Niigata Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Daisuke","family":"Yonezawa","sequence":"additional","affiliation":[{"name":"Division of Microbiology and Infectious Diseases Niigata University Graduate School of Medical and Dental Sciences Niigata Japan"},{"name":"Research Center for Advanced Oral Science Niigata University Graduate School of Medical and Dental Sciences Niigata Japan"},{"name":"Division of Oral Science for Health Promotion Niigata University Graduate School of Medical and Dental Sciences Niigata Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hikaru","family":"Tamura","sequence":"additional","affiliation":[{"name":"Division of Microbiology and Infectious Diseases Niigata University Graduate School of Medical and Dental Sciences Niigata Japan"},{"name":"Research Center for Advanced Oral Science Niigata University Graduate School of Medical and Dental Sciences Niigata Japan"},{"name":"Division of Periodontology Niigata University Graduate School of Medical and Dental Sciences Niigata Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shigetada","family":"Kawabata","sequence":"additional","affiliation":[{"name":"Department of Oral and Molecular Microbiology Osaka University, Graduate School of Dentistry Osaka Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Katsunori","family":"Yanagihara","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine Nagasaki University Graduate School of Biomedical Sciences Nagasaki Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Osamu","family":"Kimura","sequence":"additional","affiliation":[{"name":"Shiokaze Clinic Niigata Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eiji","family":"Kunitomo","sequence":"additional","affiliation":[{"name":"Central Research and Development Laboratory Kobayashi Pharmaceutical Osaka Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4450-5583","authenticated-orcid":false,"given":"Yutaka","family":"Terao","sequence":"additional","affiliation":[{"name":"Division of Microbiology and Infectious Diseases Niigata University Graduate School of Medical and Dental Sciences Niigata Japan"},{"name":"Research Center for Advanced Oral Science Niigata University Graduate School of Medical and Dental Sciences Niigata Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2019,6,26]]},"reference":[{"key":"e_1_2_8_1_2_1","doi-asserted-by":"publisher","DOI":"10.1002\/tcr.201300007"},{"key":"e_1_2_8_1_3_1","doi-asserted-by":"publisher","DOI":"10.1038\/161719a0"},{"key":"e_1_2_8_1_4_1","doi-asserted-by":"publisher","DOI":"10.1021\/jm00390a022"},{"key":"e_1_2_8_1_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jid.2016.11.044"},{"key":"e_1_2_8_1_6_1","doi-asserted-by":"publisher","DOI":"10.3109\/02713683.2016.1151530"},{"key":"e_1_2_8_1_7_1","doi-asserted-by":"publisher","DOI":"10.1139\/m73-216"},{"key":"e_1_2_8_1_8_1","first-page":"421","article-title":"Effects of heartwood inhabiting fungi on thujaplicin content and decay resistance of western redcedar (Thuja\u2010Plicata Donn)","volume":"18","author":"Kamp BJ","year":"1986","journal-title":"Wood Fiber Sci"},{"key":"e_1_2_8_1_9_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0163147"},{"key":"e_1_2_8_1_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.micres.2012.12.007"},{"key":"e_1_2_8_1_11_1","first-page":"17","article-title":"Studies of the antibacterial activity of plant extracts and their constituents against periodontopathic bacteria","volume":"31","author":"Osawa K","year":"1990","journal-title":"Bull Tokyo Dent Coll"},{"key":"e_1_2_8_1_12_1","doi-asserted-by":"publisher","DOI":"10.3314\/mmj.17.004"},{"key":"e_1_2_8_1_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.oooo.2013.05.021"},{"key":"e_1_2_8_1_14_1","doi-asserted-by":"publisher","DOI":"10.1099\/mic.0.26082-0"},{"key":"e_1_2_8_1_15_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-051X.1998.tb02419.x"},{"key":"e_1_2_8_1_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.imlet.2014.08.017"},{"key":"e_1_2_8_1_17_1","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.00653-07"},{"key":"e_1_2_8_1_18_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev-micro-092412-155711"},{"key":"e_1_2_8_1_19_1","doi-asserted-by":"publisher","DOI":"10.1001\/jama.298.15.1763"},{"key":"e_1_2_8_1_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jiac.2018.08.018"},{"key":"e_1_2_8_1_21_1","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2013.00507"},{"key":"e_1_2_8_1_22_1","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/dkp159"},{"key":"e_1_2_8_1_23_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13756-015-0096-x"},{"key":"e_1_2_8_1_24_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1473-3099(04)00938-7"},{"key":"e_1_2_8_1_25_1","doi-asserted-by":"publisher","DOI":"10.1002\/1873-3468.12254"},{"key":"e_1_2_8_1_26_1","doi-asserted-by":"publisher","DOI":"10.1002\/mbo3.308"},{"key":"e_1_2_8_1_27_1","doi-asserted-by":"publisher","DOI":"10.1111\/1348-0421.12343"},{"key":"e_1_2_8_1_28_1","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.00161-18"},{"key":"e_1_2_8_1_29_1","doi-asserted-by":"publisher","DOI":"10.1038\/srep38013"},{"key":"e_1_2_8_1_30_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.micinf.2017.09.007"},{"key":"e_1_2_8_1_31_1","doi-asserted-by":"publisher","DOI":"10.1111\/jre.12134"},{"key":"e_1_2_8_1_32_1","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2334-12-253"},{"key":"e_1_2_8_1_33_1","doi-asserted-by":"publisher","DOI":"10.4265\/bio.12.101"},{"key":"e_1_2_8_1_34_1","doi-asserted-by":"publisher","DOI":"10.1271\/bbb.80778"},{"key":"e_1_2_8_1_35_1","doi-asserted-by":"publisher","DOI":"10.1248\/bpb.22.990"},{"key":"e_1_2_8_1_36_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0923-1811(97)00070-4"},{"key":"e_1_2_8_1_37_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.107.2767.44"},{"key":"e_1_2_8_1_38_1","doi-asserted-by":"publisher","DOI":"10.1139\/W08-161"},{"key":"e_1_2_8_1_39_1","doi-asserted-by":"publisher","DOI":"10.1139\/m73-190"},{"key":"e_1_2_8_1_40_1","doi-asserted-by":"publisher","DOI":"10.1248\/bpb.31.735"},{"key":"e_1_2_8_1_41_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0171244"},{"key":"e_1_2_8_1_42_1","doi-asserted-by":"publisher","DOI":"10.1248\/bpb.16.521"},{"key":"e_1_2_8_1_43_1","doi-asserted-by":"publisher","DOI":"10.1248\/bpb.24.299"},{"key":"e_1_2_8_1_44_1","first-page":"755","article-title":"Cytotoxic activity of deferiprone, maltol and related hydroxyketones against human tumor cell lines","volume":"24","author":"Yasumoto E","year":"2004","journal-title":"Anticancer Res"}],"container-title":["Microbiology and Immunology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2F1348-0421.12688","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/1348-0421.12688","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1111\/1348-0421.12688","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/1348-0421.12688","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,10]],"date-time":"2023-09-10T10:01:29Z","timestamp":1694340089000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/1348-0421.12688"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2019,6]]},"references-count":43,"journal-issue":{"issue":"6","published-print":{"date-parts":[[2019,6]]}},"alternative-id":["10.1111\/1348-0421.12688"],"URL":"https:\/\/doi.org\/10.1111\/1348-0421.12688","archive":["Portico"],"relation":{},"ISSN":["0385-5600","1348-0421"],"issn-type":[{"value":"0385-5600","type":"print"},{"value":"1348-0421","type":"electronic"}],"subject":[],"published":{"date-parts":[[2019,6]]},"assertion":[{"value":"2019-02-07","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2019-05-06","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2019-06-26","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}