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The ATP\u2010binding cassette\u2013type drug efflux pump MacAB was originally reported as a macrolide\u2010specific pump. MacAB is also known to be required for the virulence of <jats:italic>Salmonella enterica<\/jats:italic> serovar Typhimurium following oral infection in mice. Here, we performed a screening of inhibitors of <jats:italic>Salmonella<\/jats:italic> MacAB and found a compound that increased the susceptibility of a MacAB\u2010expressing strain to macrolides. It was previously reported that MacAB is required to resist peroxide\u2010mediated killing <jats:italic>in vitro<\/jats:italic> and that a supernatant of wild\u2010type <jats:italic>Salmonella<\/jats:italic> rescues the growth defect of a <jats:italic>macAB<\/jats:italic> mutant in H<jats:sub>2<\/jats:sub>O<jats:sub>2<\/jats:sub>. In this study, we also found that the MacAB inhibitor reduced the ability of the supernatant to rescue <jats:italic>Salmonella<\/jats:italic> cells in H<jats:sub>2<\/jats:sub>O<jats:sub>2<\/jats:sub>. This compound could lead to a better understanding of the function of MacAB.<\/jats:p>","DOI":"10.1111\/1348-0421.12765","type":"journal-article","created":{"date-parts":[[2019,12,11]],"date-time":"2019-12-11T10:44:16Z","timestamp":1576061056000},"page":"182-188","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":9,"title":["An efflux inhibitor of the MacAB pump in <i>Salmonella enterica<\/i> serovar Typhimurium"],"prefix":"10.1111","volume":"64","author":[{"given":"Ami","family":"Yamagishi","sequence":"first","affiliation":[{"name":"Graduate School of Pharmaceutical Sciences Osaka University Osaka Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sohei","family":"Nakano","sequence":"additional","affiliation":[{"name":"School of Pharmaceutical Sciences Osaka University Osaka 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