{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,12,3]],"date-time":"2025-12-03T17:53:25Z","timestamp":1764784405865,"version":"3.40.4"},"reference-count":39,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2020,4,20]],"date-time":"2020-04-20T00:00:00Z","timestamp":1587340800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001700","name":"Ministry of Education, Culture, Sports, Science and Technology","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100001700","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100009619","name":"Japan Agency for Medical Research and Development","doi-asserted-by":"publisher","award":["JP18fm0108002"],"award-info":[{"award-number":["JP18fm0108002"]}],"id":[{"id":"10.13039\/100009619","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Microbiology and Immunology"],"published-print":{"date-parts":[[2020,6]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Non\u2010O1\/non\u2010O139 nontoxigenic <jats:italic>Vibrio cholerae<\/jats:italic> associated with cholera\u2010like diarrhea has been reported in Kolkata, India. However, the property involved in the pathogenicity of these strains has remained unclear. The character of 25 non\u2010O1\/non\u2010O139 nontoxigenic <jats:italic>V. cholerae<\/jats:italic> isolated during 8 years from 2007 to 2014 in Kolkata was examined. Determination of the serogroup showed that the serogroups O6, O10, O35, O36, O39, and O70 were represented by two strains in each serogroup, and the remaining isolates belonged to different serogroups. To clarify the character of antibiotic resistance of these isolates, an antibiotic resistance test and the gene analysis were performed. According to antimicrobial drug susceptibility testing, 13 strains were classified as drug resistant. Among them, 10 strains were quinolone resistant and 6 of the 13 strains were resistant to more than three antibiotics. To define the genetic background of the antibiotic character of these strains, whole\u2010genome sequences of these strains were determined. From the analysis of these sequences, it becomes clear that all quinolone resistance isolates have mutations in quinolone resistance\u2010determining regions. Further research on the genome sequence showed that four strains possess Class 1 integrons in their genomes, and that three of the four integrons are found to be located in their genomic islands. These genomic islands are novel types. This indicates that various integrons containing drug resistance genes are spreading among <jats:italic>V. cholerae<\/jats:italic> non\u2010O1\/non\u2010O139 strains through the action of newly generated genomic islands.<\/jats:p>","DOI":"10.1111\/1348-0421.12790","type":"journal-article","created":{"date-parts":[[2020,3,28]],"date-time":"2020-03-28T22:39:36Z","timestamp":1585435176000},"page":"435-444","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":17,"title":["Genomic characterization of antibiotic resistance\u2010encoding genes in clinical isolates of <i>Vibrio cholerae<\/i> non\u2010O1\/non\u2010O139 strains from Kolkata, India: generation of novel types of genomic islands containing plural antibiotic resistance genes"],"prefix":"10.1111","volume":"64","author":[{"given":"Daichi","family":"Morita","sequence":"first","affiliation":[{"name":"Collaborative Research Center of Okayama University for Infectious Diseases in India, Okayama University  Kolkata India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Eizo","family":"Takahashi","sequence":"additional","affiliation":[{"name":"Collaborative Research Center of Okayama University for Infectious Diseases in India, Okayama University  Kolkata India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Masatomo","family":"Morita","sequence":"additional","affiliation":[{"name":"Department of Bacteriology I National Institute of Infectious Diseases  Tokyo Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Makoto","family":"Ohnishi","sequence":"additional","affiliation":[{"name":"Department of Bacteriology I National Institute of Infectious Diseases  Tokyo Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tamaki","family":"Mizuno","sequence":"additional","affiliation":[{"name":"Graduate School of Medicine, Dentistry and Pharmaceutical Sciences Okayama University  Okayama Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shin\u2010ichi","family":"Miyoshi","sequence":"additional","affiliation":[{"name":"Graduate School of Medicine, Dentistry and Pharmaceutical Sciences Okayama University  Okayama Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Devarati","family":"Dutta","sequence":"additional","affiliation":[{"name":"Division of Bacteriology National Institute of Cholera and Enteric Diseases  Kolkata India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Thandavarayan","family":"Ramamurthy","sequence":"additional","affiliation":[{"name":"Center for Human Microbial Ecology Translational Health Science and Technology Institute  Faridabad India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Goutam","family":"Chowdhury","sequence":"additional","affiliation":[{"name":"Division of Bacteriology National Institute of Cholera and Enteric Diseases  Kolkata India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Asish K.","family":"Mukhopadhyay","sequence":"additional","affiliation":[{"name":"Division of Bacteriology National Institute of Cholera and Enteric Diseases  Kolkata India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0614-5492","authenticated-orcid":false,"given":"Keinosuke","family":"Okamoto","sequence":"additional","affiliation":[{"name":"Collaborative Research Center of Okayama University for Infectious Diseases in India, Okayama University  Kolkata India"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2020,4,20]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(12)60436-X"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1111\/imj.12409"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10096-013-2011-9"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1177\/003335491312800613"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1093\/cid\/cis243"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.11150\/kansenshogakuzasshi1970.71.1037"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.3201\/eid1903.121156"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.03540-14"},{"key":"e_1_2_8_10_1","first-page":"665","article-title":"A review of pathogenic vibrio infections for clinicians","volume":"17","author":"Daniels NA","year":"2000","journal-title":"Infect Med"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.2166\/wst.2004.0014"},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.38.11.4145-4151.2000"},{"volume-title":"Performance standards for antimicrobial susceptibility testing; twenty\u2010fourth informational supplement","year":"2014","author":"CLSI (Clinical and Laboratory Standards Institute)","key":"e_1_2_8_13_1"},{"volume-title":"Methods for antimicrobial dilution and disk susceptibility testing of infrequently isolated or fastidious bacteria","year":"2015","author":"CLSI (Clinical and Laboratory Standards Institute)","key":"e_1_2_8_14_1"},{"key":"e_1_2_8_15_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pntd.0005386"},{"key":"e_1_2_8_16_1","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/btp105"},{"key":"e_1_2_8_17_1","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.46.8.2676-2678.2002"},{"key":"e_1_2_8_18_1","first-page":"14","article-title":"Resistance integrons: class 1, 2 and 3 integrons","volume":"20","author":"Deng Y","year":"2015","journal-title":"Ann Clin Microbiol Antimicrob"},{"key":"e_1_2_8_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.meegid.2017.04.013"},{"key":"e_1_2_8_20_1","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.46.5.1288-1294.2002"},{"key":"e_1_2_8_21_1","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.39.10.2359"},{"key":"e_1_2_8_22_1","doi-asserted-by":"publisher","DOI":"10.1128\/mBio.00509-16"},{"key":"e_1_2_8_23_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2958.2005.04520.x"},{"key":"e_1_2_8_24_1","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/dkv137"},{"key":"e_1_2_8_25_1","doi-asserted-by":"publisher","DOI":"10.1128\/JB.00834-08"},{"key":"e_1_2_8_26_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(03)15328-7"},{"key":"e_1_2_8_27_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.meegid.2014.01.006"},{"key":"e_1_2_8_28_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tim.2015.03.010"},{"key":"e_1_2_8_29_1","doi-asserted-by":"publisher","DOI":"10.1099\/jmm.0.46339-0"},{"key":"e_1_2_8_30_1","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.44.5.1315-1321.2000"},{"key":"e_1_2_8_31_1","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.46.9.2948-2955.2002"},{"key":"e_1_2_8_32_1","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.38.10.3774-3779.2000"},{"key":"e_1_2_8_33_1","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/dku314"},{"key":"e_1_2_8_34_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.vetmic.2008.06.027"},{"key":"e_1_2_8_35_1","doi-asserted-by":"publisher","DOI":"10.4014\/jmb.1708.08040"},{"key":"e_1_2_8_36_1","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/dku371"},{"key":"e_1_2_8_37_1","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/dkv154"},{"key":"e_1_2_8_38_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijantimicag.2016.05.020"},{"key":"e_1_2_8_39_1","doi-asserted-by":"publisher","DOI":"10.1099\/jmm.0.023051-0"},{"key":"e_1_2_8_40_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2958.1999.01330.x"}],"container-title":["Microbiology and Immunology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2F1348-0421.12790","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/1348-0421.12790","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1111\/1348-0421.12790","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/am-pdf\/10.1111%2F1348-0421.12790","content-type":"application\/pdf","content-version":"am","intended-application":"syndication"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/1348-0421.12790","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,4,11]],"date-time":"2025-04-11T12:31:35Z","timestamp":1744374695000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/1348-0421.12790"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2020,4,20]]},"references-count":39,"journal-issue":{"issue":"6","published-print":{"date-parts":[[2020,6]]}},"alternative-id":["10.1111\/1348-0421.12790"],"URL":"https:\/\/doi.org\/10.1111\/1348-0421.12790","archive":["Portico"],"relation":{},"ISSN":["0385-5600","1348-0421"],"issn-type":[{"type":"print","value":"0385-5600"},{"type":"electronic","value":"1348-0421"}],"subject":[],"published":{"date-parts":[[2020,4,20]]},"assertion":[{"value":"2020-01-10","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2020-03-22","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2020-04-20","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}