{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,10]],"date-time":"2026-07-10T12:04:57Z","timestamp":1783685097142,"version":"3.55.0"},"reference-count":33,"publisher":"Wiley","issue":"11","license":[{"start":{"date-parts":[[2020,10,8]],"date-time":"2020-10-08T00:00:00Z","timestamp":1602115200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Microbiology and Immunology"],"published-print":{"date-parts":[[2020,11]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Brucellosis is a major zoonotic disease, and <jats:italic>Brucella melitensis<\/jats:italic> is the species most often associated with human infection. Vaccination is the most efficient tool for controlling animal brucellosis, with a consequent decrease of incidence of human infections. Commercially available live attenuated vaccines provide some degree of protection, but retain residual pathogenicity to human and animals. In this study, <jats:italic>Brucella ovis<\/jats:italic> \u2206<jats:italic>abcBA<\/jats:italic> (<jats:italic>Bo<\/jats:italic>\u2206<jats:italic>abcBA<\/jats:italic>), a live attenuated candidate vaccine strain, was tested in two formulations (encapsulated with alginate and alginate plus vitelline protein B [VpB]) to immunize mice against experimental challenge with <jats:italic>B. melitensis<\/jats:italic> strain 16M. One week after infection, livers and spleens of immunized mice had reduced numbers of the challenge strain <jats:italic>B. melitensis<\/jats:italic> 16M when compared with those of nonimmunized mice, with a reduction of approximately 1\u2010log<jats:sub>10<\/jats:sub> of <jats:italic>B. melitensis<\/jats:italic> 16M count in the spleens from immunized mice. Moreover, splenocytes stimulated with <jats:italic>B. melitensis<\/jats:italic> antigens <jats:italic>in vitro<\/jats:italic> secreted IFN\u2010\u03b3 when mice had been immunized with <jats:italic>Bo<\/jats:italic>\u2206<jats:italic>abcBA<\/jats:italic> encapsulated with alginate plus VpB, but not with alginate alone. Body and liver weights were similar among groups, although spleens from mice immunized with <jats:italic>Bo<\/jats:italic>\u2206<jats:italic>abcBA<\/jats:italic> encapsulated with alginate were larger than those immunized with <jats:italic>Bo<\/jats:italic>\u2206<jats:italic>abcBA<\/jats:italic> encapsulated with alginate plus VpB or nonimmunized mice. This study demonstrated that two vaccine formulations containing <jats:italic>Bo<\/jats:italic>\u2206<jats:italic>abcBA<\/jats:italic> protected mice against experimental challenge with <jats:italic>B. melitensis<\/jats:italic>.<\/jats:p>","DOI":"10.1111\/1348-0421.12850","type":"journal-article","created":{"date-parts":[[2020,9,23]],"date-time":"2020-09-23T16:30:54Z","timestamp":1600878654000},"page":"730-736","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":13,"title":["The candidate vaccine strain <i>Brucella ovis<\/i> \u2206<i>abcBA<\/i> is protective against <i>Brucella melitensis<\/i> infection in mice"],"prefix":"10.1111","volume":"64","author":[{"given":"Luciana Fachini","family":"Costa","sequence":"first","affiliation":[{"name":"Departamento de Cl\u00ednica e Cirurgia Veterin\u00e1rias, Escola de Veterin\u00e1ria Universidade Federal de Minas Gerais Belo Horizonte Minas Gerais 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