{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,20]],"date-time":"2026-10-20T16:09:51Z","timestamp":1792512591686,"version":"4.5.0"},"reference-count":40,"publisher":"Wiley","issue":"12","license":[{"start":{"date-parts":[[2020,12,1]],"date-time":"2020-12-01T00:00:00Z","timestamp":1606780800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100004564","name":"Ministarstvo Prosvete, Nauke i Tehnolo\u0161kog Razvoja","doi-asserted-by":"publisher","award":["175075"],"award-info":[{"award-number":["175075"]}],"id":[{"id":"10.13039\/501100004564","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Microbiology and Immunology"],"published-print":{"date-parts":[[2020,12]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The aim was to investigate the detection rates of periodontal bacteria (<jats:italic>Porphyromonas gingivalis, Tannerella forsythia, Prevotella intermedia<\/jats:italic>, and <jats:italic>Aggregatibacter actinomycetemcomitans<\/jats:italic>) and herpesviruses (herpes simplex virus\u20101 [HSV\u20101], cytomegalovirus [CMV], and Epstein\u2010Barr virus [EBV]) in different forms and severity of periodontal disease, and to compare them with those in periodontally healthy subjects. One hundred and twenty\u2010nine patients participated in the study: 39 diagnosed with periodontal abscess (PA), 33 with necrotizing ulcerative periodontitis (NUP), 27 with chronic periodontitis (CP), and 30 participants with healthy periodontal tissue represented a healthy control group. All patients with periodontal disease (PA, NUP, and CP) were also divided into two groups according to the severity of their disease: moderate and severe periodontitis. The subgingival samples were collected from the periodontitis active sites and the detection of microorganisms was performed by end\u2010point polymerase chain reaction analyses. The results revealed significantly higher detection rates of <jats:italic>P. gingivalis, T. forsythia<\/jats:italic>, and <jats:italic>P. intermedia<\/jats:italic> in all three groups of patients with periodontitis than in healthy participants. The highest detection rate of <jats:italic>A. actinomycetemcomitans<\/jats:italic> was noticed in CP, which was significantly higher than that in PA, NUP, and healthy control. The occurrence of EBV was significantly higher in NUP than in CP and healthy participants. CMV was detected significantly more frequently in PA and NUP than in CP and healthy participants. Comparisons among healthy participants and patients with moderate and severe periodontitis showed significantly higher detection rates of EBV and CMV in patients with severe forms of periodontitis than in healthy participants and those with moderate periodontitis.<\/jats:p>","DOI":"10.1111\/1348-0421.12857","type":"journal-article","created":{"date-parts":[[2020,10,27]],"date-time":"2020-10-27T09:48:16Z","timestamp":1603792096000},"page":"815-824","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":24,"title":["Detection rates of periodontal bacteria and herpesviruses in different forms of periodontal disease"],"prefix":"10.1111","volume":"64","author":[{"given":"Miljan","family":"Puletic","sequence":"first","affiliation":[{"name":"Clinic for Periodontology and Oral Medicine, School of Dental Medicine University of Belgrade  Belgrade Serbia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Branka","family":"Popovic","sequence":"additional","affiliation":[{"name":"Department of Human Genetics, School of Dental Medicine University of Belgrade  Belgrade Serbia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sasa","family":"Jankovic","sequence":"additional","affiliation":[{"name":"Clinic for Periodontology and Oral Medicine, School of Dental Medicine University of Belgrade  Belgrade Serbia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3311-0148","authenticated-orcid":false,"given":"Gavrilo","family":"Brajovic","sequence":"additional","affiliation":[{"name":"Department of Physiology, School of Dental Medicine University of Belgrade  Belgrade 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