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A vaccine preventing colonization and consecutive infection with MDRGNO could be particularly valuable, as therapeutic options become increasingly limited. Outer membrane vesicles (OMV) of <jats:italic>Escherichia coli<\/jats:italic> strain CFT073 as well as three MDRGNO strains that had caused severe infections in humans were administered intranasally to mice, with and without cholera toxin as an adjuvant. The humoral immune responses were comparatively matched with the sera of patients, who had suffered an infection caused by the respective bacterium. Additionally, systemic and local toxicity was evaluated. Intranasal vaccination with OMV could elicit solid humoral immune responses (total IgM and IgG), specific for the respective MDRGNO in mice; decoration of vital bacterial membranes with antibodies was comparable to patients who had survived systemic infection with the respective bacterial isolate. After intranasal vaccination of mice with OMV no signs of local or systemic toxicity were observed. Intranasal vaccination with OMV may open up a rapid vaccine approach to prevent colonization and\/or infection with pathogenic MDRGNOs, especially in an outbreak setting within a hospital. It may also be an option for patients who have to undergo elective interventions in centers with a high risk of infection for certain common MDRGNO. Future studies need to include challenge experiments as well as phase I trials in humans.<\/jats:p>","DOI":"10.1111\/1348-0421.12882","type":"journal-article","created":{"date-parts":[[2021,3,2]],"date-time":"2021-03-02T03:53:51Z","timestamp":1614657231000},"page":"214-227","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["Rapid prototyping vaccine approach in mice against multi\u2010drug resistant Gram\u2010negative organisms from clinical isolates based on outer membrane vesicles"],"prefix":"10.1111","volume":"65","author":[{"given":"Michael","family":"Pritsch","sequence":"first","affiliation":[{"name":"Department of Bacteriology Max von Pettenkofer\u2010Institute (LMU)  Munich Germany"},{"name":"Division of Infectious Diseases and Tropical Medicine, University Hospital LMU Munich  Munich Germany"},{"name":"3CIHLMU Center for International Health LMU Munich  Munich Germany"},{"name":"German Center for Infection Research (DZIF) Partner Site Munich  Munich Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9681-2542","authenticated-orcid":false,"given":"Najib","family":"Ben Khaled","sequence":"additional","affiliation":[{"name":"Department of Bacteriology Max von Pettenkofer\u2010Institute (LMU)  Munich Germany"},{"name":"Department of Medicine II University Hospital Munich (LMU)  Munich Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gabriele","family":"Liegl","sequence":"additional","affiliation":[{"name":"Department of Bacteriology Max von Pettenkofer\u2010Institute (LMU)  Munich Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Soeren","family":"Schubert","sequence":"additional","affiliation":[{"name":"Department of Bacteriology Max von Pettenkofer\u2010Institute (LMU)  Munich Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Michael","family":"Hoelscher","sequence":"additional","affiliation":[{"name":"Division of Infectious Diseases and Tropical Medicine, University Hospital LMU Munich  Munich Germany"},{"name":"3CIHLMU Center for International Health LMU Munich  Munich Germany"},{"name":"German Center for Infection Research (DZIF) Partner Site Munich  Munich Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Christine","family":"Woischke","sequence":"additional","affiliation":[{"name":"Institute of Pathology, Faculty of Medicine LMU Munich  Munich Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nathalie","family":"Arens","sequence":"additional","affiliation":[{"name":"Division of Infectious Diseases and Tropical Medicine, University Hospital LMU Munich  Munich Germany"},{"name":"Department of Medicine IV University Hospital Augsburg  Augsburg Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Julia","family":"Thorn\u2010Seshold","sequence":"additional","affiliation":[{"name":"Department of Pharmacy LMU Munich  Munich Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Stefan","family":"Kammermeier","sequence":"additional","affiliation":[{"name":"Department of Neurology University Hospital Munich (LMU)  Munich Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Andreas","family":"Wieser","sequence":"additional","affiliation":[{"name":"Department of Bacteriology Max von Pettenkofer\u2010Institute (LMU)  Munich Germany"},{"name":"Division of Infectious Diseases and Tropical Medicine, University Hospital LMU Munich  Munich Germany"},{"name":"3CIHLMU Center for International Health LMU Munich  Munich Germany"},{"name":"German Center for Infection Research (DZIF) Partner Site Munich  Munich Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2021,5,4]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1322678111"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1469-0691.2012.03821.x"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1473-3099(18)30605-4"},{"key":"e_1_2_10_5_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pmed.1002150"},{"key":"e_1_2_10_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1473-3099(08)70041-0"},{"key":"e_1_2_10_7_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1469-0691.2011.03570.x"},{"key":"e_1_2_10_8_1","doi-asserted-by":"publisher","DOI":"10.1128\/mSphere.00261-16"},{"key":"e_1_2_10_9_1","doi-asserted-by":"publisher","DOI":"10.1093\/cid\/cix270"},{"key":"e_1_2_10_10_1","doi-asserted-by":"publisher","DOI":"10.2471\/BLT.07.040089"},{"key":"e_1_2_10_11_1","unstructured":"PlotkinSA OrensteinWA OffitPA EdwardsKM.Plotkin's vaccines.2018."},{"key":"e_1_2_10_12_1","doi-asserted-by":"crossref","unstructured":"Editorial Material.Vaccines work [published online ahead of print April 24 2018]. 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