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A deep understanding of the immunopathogenesis of LN is essential to identify optimal molecular targets, as most immunotherapeutic algorithms are still based on unselective drugs. The study aimed to elucidate the possible association of vitamin D deficiency with the programmed death\u20101 (PD\u20101)\/programmed death ligand\u20101 (PD\u2010L1) axis and inflammatory response in patients with LN, as well as the relationship between the PD\u20101\/PD\u2010L1 axis and chemokine C\u2010X\u2010C motif ligand 12 (CXCL12). Flow cytometry was used to determine the frequencies of CD279 (PD\u20101) and CD274 (PD\u2010L1) in the peripheral CD3+CD4+ cell population of persons with LN. Furthermore, ELISA was used to detect serum CXCL12 and vitamin D concentrations. A distinct decrease of PD\u20101 and a significant increase of PD\u2010L1 was demonstrated in patients with LN compared with either SLE patients with no LN or healthy controls. The PD\u20101\/PD\u2010L1 axis was negatively correlated with different disease parameters. Vitamin D deficiency and insufficiency were more prevalent in patients with LN than in controls, being significantly associated with disease activity and inversely associated with the PD\u20101\/PD\u2010L1 expression. Moreover, CXCL12 was negatively correlated with the PD\u20101\/PD\u2010L1 axis and vitamin D concentration. The findings suggest an involvement of the PD\u20101\/PD\u2010L1 axis in lupus nephritis, which might serve as a potential highly selective therapeutic target that is more effective but less toxic. In addition, restoring adequate vitamin D levels in lupus nephritis could be a possible simple measure to control inflammatory immune responses.<\/jats:p>","DOI":"10.1111\/1348-0421.12923","type":"journal-article","created":{"date-parts":[[2021,6,3]],"date-time":"2021-06-03T20:16:19Z","timestamp":1622751379000},"page":"392-399","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["The immunoregulatory axis (programmed death\u20101\/programmed death ligand\u20101) on CD4+ T cells in lupus nephritis: association with vitamin D and chemokine C\u2010X\u2010C motif ligand 12"],"prefix":"10.1111","volume":"65","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-3202-4838","authenticated-orcid":false,"given":"Sara","family":"Youssry","sequence":"first","affiliation":[{"name":"Department of Immunology and Allergy, Medical Research Institute Alexandria University  Alexandria Egypt"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Amina","family":"Hussein","sequence":"additional","affiliation":[{"name":"Department of Immunology and Allergy, Medical Research Institute Alexandria University  Alexandria Egypt"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5854-7736","authenticated-orcid":false,"given":"Mai","family":"Moaaz","sequence":"additional","affiliation":[{"name":"Department of Immunology and Allergy, Medical Research Institute Alexandria University  Alexandria Egypt"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2021,7,16]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrneph.2015.33"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.1097\/MAJ.0b013e31827f4ee3"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.1093\/ndt\/gfs073"},{"key":"e_1_2_10_5_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12519-019-00229-3"},{"key":"e_1_2_10_6_1","doi-asserted-by":"publisher","DOI":"10.3390\/cells8101213"},{"key":"e_1_2_10_7_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1749-6632.2010.05919.x"},{"key":"e_1_2_10_8_1","doi-asserted-by":"publisher","DOI":"10.3389\/fmed.2018.00351"},{"key":"e_1_2_10_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cellimm.2014.05.006"},{"key":"e_1_2_10_10_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M117.793885"},{"key":"e_1_2_10_11_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00403-020-02094-x"},{"key":"e_1_2_10_12_1","doi-asserted-by":"publisher","DOI":"10.18632\/oncotarget.15489"},{"key":"e_1_2_10_13_1","doi-asserted-by":"publisher","DOI":"10.1186\/s40425-018-0344-8"},{"key":"e_1_2_10_14_1","doi-asserted-by":"publisher","DOI":"10.1002\/eji.200839103"},{"key":"e_1_2_10_15_1","doi-asserted-by":"publisher","DOI":"10.3892\/mmr.2013.1874"},{"key":"e_1_2_10_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ctrv.2018.05.016"},{"key":"e_1_2_10_17_1","doi-asserted-by":"crossref","unstructured":"SinghRK KimK Rowswell\u2010TurnerRB et al.Targeting Vitamin\u2010D receptor (VDR) by a small molecule antagonist MeTC7 inhibits PD\u2010L1 but controls THMYCN neuroblastoma growth PD\u2010L1 independently. bioRxiv. 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