{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,18]],"date-time":"2025-10-18T21:01:03Z","timestamp":1760821263771,"version":"3.37.3"},"reference-count":41,"publisher":"Wiley","issue":"12","license":[{"start":{"date-parts":[[2021,11,15]],"date-time":"2021-11-15T00:00:00Z","timestamp":1636934400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001691","name":"Japan Society for the Promotion of Science","doi-asserted-by":"publisher","award":["KAKENHI Grant Numbers 20K09926"],"award-info":[{"award-number":["KAKENHI Grant Numbers 20K09926"]}],"id":[{"id":"10.13039\/501100001691","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Microbiology and Immunology"],"published-print":{"date-parts":[[2021,12]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p><jats:italic>Treponema denticola<\/jats:italic>, a helically shaped motile microorganism, is a major pathogen of chronic periodontitis. Major surface protein (Msp) and dentilisin are virulence factors of <jats:italic>T. denticola<\/jats:italic> that are located on the outer sheath. The motility of <jats:italic>T. denticola<\/jats:italic> is deeply involved in colonization on and invasion into the host tissue. The outer sheath is located at the interface between the environment and <jats:italic>T. denticola<\/jats:italic>, and its components may also contribute to its motility via interaction with the materials outside the cells. The study aimed to clarify whether Msp or dentilisin contributes to the motility of <jats:italic>T. denticola<\/jats:italic> on solid surfaces, termed crawling, by investigating their effects using Msp\u2010deficient and dentilisin\u2010deficient <jats:italic>T. denticola<\/jats:italic> strains. Motility was analyzed by measuring the colony size in agar plates and velocity was analyzed using dark\u2010field microscopy. The colony area of the mutant strains was smaller than that of the wild\u2010type strain. The crawling velocity of the mutant strains was lower than that of the wild\u2010type strain, with the lowest velocity observed in the dentilisin\u2010deficient strain. Additionally, the ratio of the crawling distance by one revolution to the protoplasmic cylinder pitch (an indicator of the crawling efficiency) in the dentilisin mutant was significantly lower than that in the wild type strain and the Msp mutant. Together, these results indicate that dentilisin facilitates the crawling\u2010dependent surface spreading of <jats:italic>T. denticola<\/jats:italic>.<\/jats:p>","DOI":"10.1111\/1348-0421.12940","type":"journal-article","created":{"date-parts":[[2021,9,9]],"date-time":"2021-09-09T13:15:46Z","timestamp":1631193346000},"page":"551-558","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":10,"title":["Crawling motility of <i>Treponema denticola<\/i> modulated by outer sheath protein"],"prefix":"10.1111","volume":"65","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-3662-1234","authenticated-orcid":false,"given":"Eitoyo","family":"Kokubu","sequence":"first","affiliation":[{"name":"Department of Microbiology Tokyo Dental College  Chiyoda\u2010ku Tokyo Japan"},{"name":"Oral Health Science Center Tokyo Dental College  Chiyoda\u2010ku Tokyo Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yuichiro","family":"Kikuchi","sequence":"additional","affiliation":[{"name":"Department of Microbiology Tokyo Dental College  Chiyoda\u2010ku Tokyo Japan"},{"name":"Oral Health Science Center Tokyo Dental College  Chiyoda\u2010ku Tokyo Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kazuko","family":"Okamoto\u2010Shibayama","sequence":"additional","affiliation":[{"name":"Department of Microbiology Tokyo Dental College  Chiyoda\u2010ku Tokyo Japan"},{"name":"Oral Health Science Center Tokyo Dental College  Chiyoda\u2010ku Tokyo Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3972-7779","authenticated-orcid":false,"given":"Shuichi","family":"Nakamura","sequence":"additional","affiliation":[{"name":"Department of Applied Physics, Graduate School of Engineering Tohoku University  Sendai Miyagi Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6567-807X","authenticated-orcid":false,"given":"Kazuyuki","family":"Ishihara","sequence":"additional","affiliation":[{"name":"Department of Microbiology Tokyo Dental College  Chiyoda\u2010ku Tokyo Japan"},{"name":"Oral Health Science Center Tokyo Dental College  Chiyoda\u2010ku Tokyo Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2021,11,15]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0757.2005.00108.x"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-051X.1998.tb02419.x"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.69.10.6276-6283.2001"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1128\/MMBR.42.1.114-160.1978"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1529\/biophysj.105.074336"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.3389\/fcimb.2019.00432"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1128\/jb.178.9.2489-2497.1996"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1128\/iai.60.5.2058-2065.1992"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.1128\/iai.64.12.5178-5186.1996"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.1128\/iai.56.10.2717-2722.1988"},{"key":"e_1_2_9_12_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.2041-1014.2010.00598.x"},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.66.5.1869-1877.1998"},{"key":"e_1_2_9_14_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0023736"},{"key":"e_1_2_9_15_1","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.63.9.3401-3410.1995"},{"key":"e_1_2_9_16_1","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.00258-07"},{"key":"e_1_2_9_17_1","doi-asserted-by":"publisher","DOI":"10.1126\/sciadv.aar7975"},{"key":"e_1_2_9_18_1","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2020.01886"},{"key":"e_1_2_9_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.micpath.2016.11.021"},{"key":"e_1_2_9_20_1","first-page":"501","volume-title":"Bergey's Manual of Systematic Bacteriology","author":"Norris SJ","year":"2010"},{"key":"e_1_2_9_21_1","doi-asserted-by":"publisher","DOI":"10.1128\/JB.180.15.3837-3844.1998"},{"key":"e_1_2_9_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.anaerobe.2014.08.008"},{"key":"e_1_2_9_23_1","doi-asserted-by":"publisher","DOI":"10.1128\/iai.63.4.1147-1152.1995"},{"key":"e_1_2_9_24_1","doi-asserted-by":"publisher","DOI":"10.1016\/0076-6879(94)28019-3"},{"key":"e_1_2_9_25_1","doi-asserted-by":"publisher","DOI":"10.1038\/nmeth.2019"},{"key":"e_1_2_9_26_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1574-6968.1998.tb13325.x"},{"key":"e_1_2_9_27_1","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.62.6.2214-2221.1994"},{"key":"e_1_2_9_28_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0506114102"},{"key":"e_1_2_9_29_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1574-6968.2001.tb10662.x"},{"key":"e_1_2_9_30_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0006-3495(95)80089-5"},{"key":"e_1_2_9_31_1","doi-asserted-by":"publisher","DOI":"10.1080\/20002297.2020.1829404"},{"key":"e_1_2_9_32_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.anaerobe.2015.09.010"},{"key":"e_1_2_9_33_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.2041-1014.2010.00584.x"},{"key":"e_1_2_9_34_1","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.68.4.1884-1892.2000"},{"key":"e_1_2_9_35_1","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.73.2.1252-1255.2005"},{"key":"e_1_2_9_36_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41594-020-0497-2"},{"key":"e_1_2_9_37_1","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.67.2.694-699.1999"},{"key":"e_1_2_9_38_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1399-302X.2007.00417.x"},{"key":"e_1_2_9_39_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.75.11.5548"},{"key":"e_1_2_9_40_1","doi-asserted-by":"publisher","DOI":"10.1084\/jem.161.3.514"},{"key":"e_1_2_9_41_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bpj.2009.02.066"},{"key":"e_1_2_9_42_1","doi-asserted-by":"publisher","DOI":"10.1529\/biophysj.106.103143"}],"container-title":["Microbiology and Immunology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/1348-0421.12940","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1111\/1348-0421.12940","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/1348-0421.12940","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,8,28]],"date-time":"2023-08-28T14:36:19Z","timestamp":1693233379000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/1348-0421.12940"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2021,11,15]]},"references-count":41,"journal-issue":{"issue":"12","published-print":{"date-parts":[[2021,12]]}},"alternative-id":["10.1111\/1348-0421.12940"],"URL":"https:\/\/doi.org\/10.1111\/1348-0421.12940","archive":["Portico"],"relation":{},"ISSN":["0385-5600","1348-0421"],"issn-type":[{"type":"print","value":"0385-5600"},{"type":"electronic","value":"1348-0421"}],"subject":[],"published":{"date-parts":[[2021,11,15]]},"assertion":[{"value":"2021-07-12","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2021-09-06","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2021-11-15","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}