{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,2,21]],"date-time":"2025-02-21T14:57:17Z","timestamp":1740149837231,"version":"3.37.3"},"reference-count":11,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2021,10,21]],"date-time":"2021-10-21T00:00:00Z","timestamp":1634774400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001691","name":"Japan Society for the Promotion of Science","doi-asserted-by":"publisher","award":["16K15277"],"award-info":[{"award-number":["16K15277"]}],"id":[{"id":"10.13039\/501100001691","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100009619","name":"Japan Agency for Medical Research and Development","doi-asserted-by":"publisher","award":["JP20fk0108129","JP21fk0108090","JP21fk0108607"],"award-info":[{"award-number":["JP20fk0108129","JP21fk0108090","JP21fk0108607"]}],"id":[{"id":"10.13039\/100009619","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Microbiology and Immunology"],"published-print":{"date-parts":[[2022,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p><jats:italic>Mycobacterium tuberculosis<\/jats:italic>, the causative agent of tuberculosis, possess flavin\u2010dependent thymidylate synthase, ThyX. Since <jats:italic>thyX<\/jats:italic> is absent in humans and was shown to be essential for <jats:italic>M. tuberculosis<\/jats:italic> normal growth, ThyX is thought to be an attractive novel TB drug target. This study assessed <jats:italic>thyX<\/jats:italic> essentiality in <jats:italic>Mycobacterium bovis<\/jats:italic> BCG strains using CRISPR interference based gene silencing and found that <jats:italic>thyX<\/jats:italic> is not essential in an <jats:italic>M. bovis<\/jats:italic> BCG Tokyo derivative strain. A <jats:italic>thyX<\/jats:italic> deletion mutant strain was successfully constructed from that strain, which reinforces the non\u2010essentiality of <jats:italic>thyX<\/jats:italic> under a certain genetic background.<\/jats:p>","DOI":"10.1111\/1348-0421.12944","type":"journal-article","created":{"date-parts":[[2021,9,21]],"date-time":"2021-09-21T14:16:55Z","timestamp":1632233815000},"page":"10-14","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Attempt of <i>thyX<\/i> gene silencing and construction of a <i>thyX<\/i> deleted clone in a <i>Mycobacterium bovis<\/i> BCG"],"prefix":"10.1111","volume":"66","author":[{"given":"Yuki","family":"Arimura","sequence":"first","affiliation":[{"name":"Department of Oral and Maxillofacial Reconstructive Surgery Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences  Okayama Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yusuke","family":"Minato","sequence":"additional","affiliation":[{"name":"Department of Microbiology Fujita Health University School of Medicine  Toyoake Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Takayuki","family":"Wada","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Graduate School of Human Life Science Osaka City University  Osaka Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Masaaki","family":"Nakayama","sequence":"additional","affiliation":[{"name":"Department of Oral Microbiology Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences  Okayama Japan"},{"name":"Advanced Research Center for Oral and Craniofacial Sciences Dental School, Okayama University  Okayama Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ayako","family":"Ryumon","sequence":"additional","affiliation":[{"name":"Department of Oral Microbiology Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences  Okayama Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nao","family":"Hirata","sequence":"additional","affiliation":[{"name":"Department of Microbiology Fujita Health University School of Medicine  Toyoake Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chie","family":"Nakajima","sequence":"additional","affiliation":[{"name":"Division of Bioresources Hokkaido University International Institute for Zoonosis Control  Sapporo Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yasuhiko","family":"Suzuki","sequence":"additional","affiliation":[{"name":"Division of Bioresources Hokkaido University International Institute for Zoonosis Control  Sapporo Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Manabu","family":"Ato","sequence":"additional","affiliation":[{"name":"Department of Mycobacteriology Leprosy Research Center, National Institute of Infectious Diseases  Tokyo Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kazuo","family":"Kobayashi","sequence":"additional","affiliation":[{"name":"Department of Immunology National Institute of Infectious Diseases  Tokyo Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Naoko","family":"Ohara","sequence":"additional","affiliation":[{"name":"Department of Operative Dentistry Okayama University Hospital, Okayama University  Okayama Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Seiji","family":"Iida","sequence":"additional","affiliation":[{"name":"Department of Oral and Maxillofacial Reconstructive Surgery Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences  Okayama Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3486-2373","authenticated-orcid":false,"given":"Naoya","family":"Ohara","sequence":"additional","affiliation":[{"name":"Department of Oral Microbiology Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences  Okayama Japan"},{"name":"Advanced Research Center for Oral and Craniofacial Sciences Dental School, Okayama University  Okayama Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2021,10,21]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.1072113"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.bi.64.070195.003445"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2958.2004.04120.x"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.1099\/mic.0.053983-0"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.1002\/cmdc.201300146"},{"key":"e_1_2_6_7_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1006939"},{"key":"e_1_2_6_8_1","doi-asserted-by":"publisher","DOI":"10.1038\/nmicrobiol.2016.274"},{"key":"e_1_2_6_9_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2958.1996.tb02533.x"},{"key":"e_1_2_6_10_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-1-4939-0554-6_12"},{"key":"e_1_2_6_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1438-4221(00)80083-1"},{"key":"e_1_2_6_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.rvsc.2014.02.009"}],"container-title":["Microbiology and Immunology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/1348-0421.12944","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1111\/1348-0421.12944","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/1348-0421.12944","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,8,28]],"date-time":"2023-08-28T10:02:57Z","timestamp":1693216977000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/1348-0421.12944"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2021,10,21]]},"references-count":11,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2022,1]]}},"alternative-id":["10.1111\/1348-0421.12944"],"URL":"https:\/\/doi.org\/10.1111\/1348-0421.12944","archive":["Portico"],"relation":{},"ISSN":["0385-5600","1348-0421"],"issn-type":[{"type":"print","value":"0385-5600"},{"type":"electronic","value":"1348-0421"}],"subject":[],"published":{"date-parts":[[2021,10,21]]},"assertion":[{"value":"2021-08-26","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2021-09-17","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2021-10-21","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}