{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,27]],"date-time":"2026-02-27T02:37:53Z","timestamp":1772159873005,"version":"3.50.1"},"reference-count":79,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2022,3,31]],"date-time":"2022-03-31T00:00:00Z","timestamp":1648684800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Microbiology and Immunology"],"published-print":{"date-parts":[[2022,5]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    <jats:italic>Pneumocystis<\/jats:italic>\n                    spp. interacts with epithelial cells in the alveolar spaces of the lung. It is thought that the binding of\n                    <jats:italic>Pneumocystis<\/jats:italic>\n                    to host cell epithelium is needed for life cycle completion and proliferation. The effect of this interaction on lung epithelial cells has previously shown that the trophic form of this organism greatly inhibits p34\n                    <jats:sup>\n                      <jats:italic>cdc2<\/jats:italic>\n                    <\/jats:sup>\n                    activity, a serine\/threonine kinase required for transition from the G\n                    <jats:sub>2<\/jats:sub>\n                    to M phase in the cell cycle. To gain further insight into the host response during\n                    <jats:italic>Pneumocystis pneumonia<\/jats:italic>\n                    infection, we used microarray technology to profile epithelial cell (A549) gene expression patterns following\n                    <jats:italic>Pneumocystis carinii<\/jats:italic>\n                    interaction. Furthermore, we isolated separate populations of cyst and trophic forms of\n                    <jats:italic>P. carinii<\/jats:italic>\n                    , which were then applied to the lung epithelial cells. Differential expression of genes involved in various cellular functions dependent on the specific\n                    <jats:italic>P. carinii<\/jats:italic>\n                    life form in contact with the A549 cell was identified. The reliability of our data was further confirmed by Northern blot analysis on a number of selected upregulated or downregulated transcripts. The transcriptional response to\n                    <jats:italic>P. carinii<\/jats:italic>\n                    was dominated by cytokines, apoptotic, and antiapoptosis\u2010related genes. These results reveal several previously unknown effects of\n                    <jats:italic>P. carinii<\/jats:italic>\n                    on the lung epithelial cell and provide insight into the complex interactions of host and pathogen.\n                  <\/jats:p>","DOI":"10.1111\/1348-0421.12972","type":"journal-article","created":{"date-parts":[[2022,3,1]],"date-time":"2022-03-01T04:44:48Z","timestamp":1646109888000},"page":"238-251","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Gene expression in lung epithelial cells following interaction with\n                    <i>Pneumocystis carinii<\/i>\n                    and its specific life forms yields insights into host gene responses to infection"],"prefix":"10.1111","volume":"66","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-0364-3311","authenticated-orcid":false,"given":"Theodore J.","family":"Kottom","sequence":"first","affiliation":[{"name":"Thoracic Diseases Research Unit, Department of Medicine Mayo Clinic College of Medicine Rochester Minnesota USA"},{"name":"Thoracic Diseases Research Unit, Department of Biochemistry Mayo Clinic College of Medicine Rochester Minnesota USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Eva M.","family":"Carmona","sequence":"additional","affiliation":[{"name":"Thoracic Diseases Research Unit, Department of Medicine Mayo Clinic College of Medicine Rochester Minnesota USA"},{"name":"Thoracic Diseases Research Unit, Department of Biochemistry Mayo Clinic College of Medicine Rochester Minnesota USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Andrew H.","family":"Limper","sequence":"additional","affiliation":[{"name":"Thoracic Diseases Research Unit, Department of Medicine Mayo Clinic College of Medicine Rochester Minnesota USA"},{"name":"Thoracic Diseases Research Unit, Department of Biochemistry Mayo Clinic College of Medicine Rochester Minnesota USA"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2022,3,31]]},"reference":[{"key":"e_1_2_11_2_1","unstructured":"TruongJ AshurstJV.Pneumocystis jiroveciipneumonia. 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