{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,6]],"date-time":"2026-06-06T01:14:09Z","timestamp":1780708449106,"version":"3.54.1"},"reference-count":21,"publisher":"Wiley","issue":"9","license":[{"start":{"date-parts":[[2022,7,18]],"date-time":"2022-07-18T00:00:00Z","timestamp":1658102400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Microbiology and Immunology"],"published-print":{"date-parts":[[2022,9]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>High measles\u2010specific antibody titers in the cerebrospinal fluid (CSF) have important diagnostic significance for subacute sclerosing panencephalitis (SSPE), a progressive neurological disorder caused by measles virus variants. However, the diagnostic reference value of antibody levels and the usefulness of the CSF\/serum ratio measured using enzyme immunoassays (EIAs) for SSPE diagnosis remain unclear. To facilitate SSPE diagnosis using EIAs, measles immunoglobulin G (IgG) titers in the CSF and serum of patients with and without SSPE were measured and their CSF\/serum antibody ratios evaluated. Serum and CSF antibody levels were compared among three patients with SSPE (59 paired samples), 37 non\u2010SSPE patients, and 2618 patients of unknown backgrounds. Of the 59 paired samples from three patients with SSPE, 56 paired samples (94.9%) showed CSF measles IgG levels \u22650.5 IU\/mL and a CSF\/serum ratio \u22650.05, whereas non\u2010SSPE cases showed CSF measles IgG levels &lt;0.1 IU\/mL and a CSF\/serum ratio &lt;0.03. Of the 2618 CSF samples with unknown backgrounds, 951 showed measurable IgG levels with EIA, with a CSF\/serum ratio peak of 0.005\u20130.02, with a 90th percentile of 0.05. Assuming the SSPE criteria as CSF measles IgG \u22650.5 IU\/mL and a CSF\/serum ratio \u22650.05, only 20 samples (0.8%) with unknown backgrounds were categorized as having SSPE. Conversely, assuming the non\u2010SSPE criteria as CSF measles IgG &lt;0.1 IU\/mL and a CSF\/serum ratio &lt;0.03, 2403 samples (92%) with unknown backgrounds were categorized as not having SSPE. In conclusion, high CSF\/serum ratios (\u22650.05) and high measles CSF IgG levels (\u22650.5 IU\/mL) may be useful for diagnosing SSPE.<\/jats:p>","DOI":"10.1111\/1348-0421.13017","type":"journal-article","created":{"date-parts":[[2022,6,29]],"date-time":"2022-06-29T11:53:51Z","timestamp":1656503631000},"page":"418-425","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Diagnostic reference value of antibody levels measured using enzyme immunoassay for subacute sclerosing panencephalitis"],"prefix":"10.1111","volume":"66","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-2985-1282","authenticated-orcid":false,"given":"Yohei","family":"Kume","sequence":"first","affiliation":[{"name":"Department of Pediatrics Fukushima Medical University 1 Hikarigaoka Fukushima Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Koichi","family":"Hashimoto","sequence":"additional","affiliation":[{"name":"Department of Pediatrics Fukushima Medical University 1 Hikarigaoka Fukushima Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Keiji","family":"Iida","sequence":"additional","affiliation":[{"name":"H.U. 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