{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,11]],"date-time":"2026-10-11T03:34:00Z","timestamp":1791689640547,"version":"4.3.4"},"reference-count":34,"publisher":"Wiley","issue":"9","license":[{"start":{"date-parts":[[2022,7,18]],"date-time":"2022-07-18T00:00:00Z","timestamp":1658102400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Microbiology and Immunology"],"published-print":{"date-parts":[[2022,9]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Dengue is a mosquito\u2010borne viral illness that infects humans. For the past few decades, it has been declared a global public health problem. The current study, conducted at the district headquarter hospital (DHQ) Bannu between June to September 2018, was based on the seroprevalence of antibodies against dengue virus serotypes and their hematological parameters among the patients. A total of 1738 individuals suspected of having dengue were diagnosed through NS1, IgG, and IgM antibodies and RT\u2010PCR techniques. Of all the samples, 716 (41.19%) were found to be positive for dengue. A higher infection rate was found in males (65.92%) compared with females (34.07%). The most affected age group was 16\u201340 years, whereas the most affected tehsil was Bannu, where the DENV\u20103 serotype was prevalent. The rare serotype (DENV\u20104) was found in 1% of cases. Symptoms including fever (100%), myalgia (100%), headache (61.31%), vomiting (34.63%), and rashes were common among the dengue patients. However, the mild cases showed fewer clinical signs compared with the severely infected cases. The study also revealed a significant association (<jats:italic>P<\/jats:italic>\u2009&lt;\u20090.05) between hematological parameters and dengue infection, showing a significant decrease in TC, eosinophils, neutrophils, and platelets and a significant increase in monocytes and lymphocytes. Based on the current report, it is concluded that patients with the above symptoms and hematological changes may have an increased probability of dengue and should be kept under observation to separate dengue\u2010positive patients and to enhance the treatment process.<\/jats:p>","DOI":"10.1111\/1348-0421.13018","type":"journal-article","created":{"date-parts":[[2022,6,30]],"date-time":"2022-06-30T06:18:15Z","timestamp":1656569895000},"page":"426-432","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":17,"title":["Epidemiological and hematological investigation of dengue virus infection"],"prefix":"10.1111","volume":"66","author":[{"given":"Faheem","family":"Anwar","sequence":"first","affiliation":[{"name":"Department of Biotechnology and Genetic Engineering Hazara University Mansehra 21300 KP Pakistan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sami","family":"Ullah","sequence":"additional","affiliation":[{"name":"Department of Biotechnology and Genetic Engineering Hazara University Mansehra 21300 KP Pakistan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3706-393X","authenticated-orcid":false,"given":"Aziz ur Rehman","family":"Aziz","sequence":"additional","affiliation":[{"name":"School of Biomedical Engineering Dalian University of Technology Dalian China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Abid Ur","family":"Rehman","sequence":"additional","affiliation":[{"name":"Department of Biotechnology and Genetic Engineering Hazara University Mansehra 21300 KP Pakistan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jawad","family":"Khan","sequence":"additional","affiliation":[{"name":"Department of Biotechnology and Genetic Engineering Hazara University Mansehra 21300 KP Pakistan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Muhammad","family":"Tayyab","sequence":"additional","affiliation":[{"name":"Institute of Biotechnology and Genetic Engineering The University of Agriculture Peshawar KP Pakistan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ihteshamul","family":"Haq","sequence":"additional","affiliation":[{"name":"Department of Biotechnology and Genetic Engineering Hazara University Mansehra 21300 KP Pakistan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Muhammad Tariq","family":"Mahmood","sequence":"additional","affiliation":[{"name":"Department of Zoology Cholistan University of Veterinary and Animal Sciences Bahawalpur Pakistan"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2022,7,18]]},"reference":[{"key":"e_1_2_11_2_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0012505"},{"key":"e_1_2_11_3_1","doi-asserted-by":"publisher","DOI":"10.1086\/651268"},{"key":"e_1_2_11_4_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12889-018-5676-2"},{"key":"e_1_2_11_5_1","first-page":"25","article-title":"Dengue infection","volume":"1","author":"Guzman MG","year":"2016","journal-title":"Nat Rev Dis Primers"},{"key":"e_1_2_11_6_1","first-page":"178","article-title":"An epidemic of Dengue fever in Karachi\u2010\u2010associated clinical manifestations","volume":"47","author":"Qureshi JA","year":"1997","journal-title":"Journal of Pakistan Medical Association"},{"key":"e_1_2_11_7_1","doi-asserted-by":"publisher","DOI":"10.4084\/mjhid.2013.014"},{"key":"e_1_2_11_8_1","unstructured":"ChitraC.Serodiagnosis of Dengue in Paediatric Patients Importance of NS1 Antigen (Doctoral dissertation.Tirunelveli Medical College.2013"},{"key":"e_1_2_11_9_1","first-page":"8","article-title":"Severity of acute hepatitis and its outcome in patients with dengue fever in a tertiary care hospital Karachi, Pakistan (South Asia)","volume":"1","author":"Parkash O","year":"2010","journal-title":"BMC Gastroenterol"},{"key":"e_1_2_11_10_1","first-page":"3","article-title":"An unusual cause of acute abdominal pain is dengue fever","volume":"819","author":"Waseem T","year":"2014","journal-title":"Am J Emerg Med"},{"key":"e_1_2_11_11_1","first-page":"8","article-title":"Severity of acute hepatitis and its outcome in patients with dengue fever in a tertiary care hospital Karachi, Pakistan (South Asia)","volume":"1","author":"Parkash O","year":"2010","journal-title":"BMC Gastroenterol"},{"key":"e_1_2_11_12_1","first-page":"1","article-title":"Dengue outbreak 2013: Clinical profile of patients presenting at DHQ Burner and THQ Shangla, Khyber Pakhtunkhwa, Pakistan","volume":"3","author":"Khan J","year":"2015","journal-title":"Immun Dis"},{"key":"e_1_2_11_13_1","unstructured":"PervinM TabassumS AliM et al. 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