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This method was able to reduce dramatically the time for full\u2010genome construction, which was used very efficiently for virus rescue, from several days or more to ~2 days, with a similar accuracy and yield to the conventional method using <jats:italic>E. coli<\/jats:italic>\/plasmid.<\/jats:p>","DOI":"10.1111\/1348-0421.13032","type":"journal-article","created":{"date-parts":[[2022,10,19]],"date-time":"2022-10-19T06:45:01Z","timestamp":1666161901000},"page":"44-47","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Demonstration of bacterium\u2010free very rapid reverse genetics system using mumps virus"],"prefix":"10.1111","volume":"67","author":[{"given":"Chaewon","family":"Bae","sequence":"first","affiliation":[{"name":"Department of Virology III National Institute of Infectious Diseases Tokyo 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