{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,2,21]],"date-time":"2025-02-21T14:57:32Z","timestamp":1740149852882,"version":"3.37.3"},"reference-count":29,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2023,3,15]],"date-time":"2023-03-15T00:00:00Z","timestamp":1678838400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001691","name":"Japan Society for the Promotion of Science","doi-asserted-by":"publisher","award":["KAKENHI [JP19K07548]","[JP22K070602]"],"award-info":[{"award-number":["KAKENHI [JP19K07548]","[JP22K070602]"]}],"id":[{"id":"10.13039\/501100001691","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100009619","name":"Japan Agency for Medical Research and Development","doi-asserted-by":"publisher","award":["JP21lm0203005"],"award-info":[{"award-number":["JP21lm0203005"]}],"id":[{"id":"10.13039\/100009619","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Microbiology and Immunology"],"published-print":{"date-parts":[[2023,5]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p><jats:italic>Bartonella elizabethae<\/jats:italic> is a rat\u2010borne zoonotic bacterium that causes human infectious endocarditis or neuroretinitis. Recently, a case of bacillary angiomatosis (BA) resulting from this organism was reported, leading to speculation that <jats:italic>B. elizabethae<\/jats:italic> may also trigger vasoproliferation. However, there are no reports of <jats:italic>B. elizabethae<\/jats:italic> promoting human vascular endothelial cell (EC) proliferation or angiogenesis, and to date, the effects of this bacterium on ECs are unknown. We recently identified a proangiogenic autotransporter, BafA, secreted from <jats:italic>B. henselae<\/jats:italic> and <jats:italic>B. quintana<\/jats:italic>, which are recognized as <jats:italic>Bartonella<\/jats:italic> spp. responsible for BA in humans. Here, we hypothesized that <jats:italic>B. elizabethae<\/jats:italic> also harbored a functional <jats:italic>bafA<\/jats:italic> gene and examined the proangiogenic activity of recombinant <jats:italic>B. elizabethae<\/jats:italic>\u2013derived BafA. The <jats:italic>bafA<\/jats:italic> gene of <jats:italic>B. elizabethae<\/jats:italic>, which was found to share a 51.1% amino acid sequence identity with BafA of <jats:italic>B. henselae<\/jats:italic> and 52.5% with that of <jats:italic>B. quintana<\/jats:italic> in the passenger domain, was located in a syntenic region of the genome. The recombinant protein of the N\u2010terminal passenger domain of <jats:italic>B. elizabethae<\/jats:italic>\u2010BafA facilitated EC proliferation and capillary structure formation. Furthermore, it upregulated the receptor signaling pathway of vascular endothelial growth factor, as observed in <jats:italic>B. henselae<\/jats:italic>\u2010BafA. Taken together, <jats:italic>B. elizabethae<\/jats:italic>\u2013derived BafA stimulates human EC proliferation and may contribute to the proangiogenic potential of this bacterium. So far, functional <jats:italic>bafA<\/jats:italic> genes have been found in all BA\u2010causing <jats:italic>Bartonella<\/jats:italic> spp., supporting the key role BafA may play in BA pathogenesis.<\/jats:p>","DOI":"10.1111\/1348-0421.13057","type":"journal-article","created":{"date-parts":[[2023,2,22]],"date-time":"2023-02-22T10:08:03Z","timestamp":1677060483000},"page":"248-257","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["The autotransporter BafA contributes to the proangiogenic potential of <i>Bartonella elizabethae<\/i>"],"prefix":"10.1111","volume":"67","author":[{"given":"Natsumi","family":"Suzuki","sequence":"first","affiliation":[{"name":"Department of Microbiology Fujita Health University School of Medicine Toyoake Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kayo","family":"Kumadaki","sequence":"additional","affiliation":[{"name":"Department of Microbiology Fujita Health University School of Medicine Toyoake Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kaoru","family":"Tatematsu","sequence":"additional","affiliation":[{"name":"Department of Microbiology Fujita Health University School of Medicine Toyoake Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yohei","family":"Doi","sequence":"additional","affiliation":[{"name":"Department of Microbiology Fujita Health University School of Medicine Toyoake Japan"},{"name":"Department of Infectious Diseases Fujita Health University School of Medicine Toyoake Japan"},{"name":"Division of Infectious Diseases University of Pittsburgh School of Medicine Pittsburgh Pennsylvania USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9254-4632","authenticated-orcid":false,"given":"Kentaro","family":"Tsukamoto","sequence":"additional","affiliation":[{"name":"Department of Microbiology Fujita Health University School of Medicine Toyoake Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2023,3,15]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.3201\/eid2303.161476"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13071-019-3390-2"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijantimicag.2014.04.006"},{"key":"e_1_2_10_5_1","doi-asserted-by":"publisher","DOI":"10.1155\/2012\/612809"},{"key":"e_1_2_10_6_1","doi-asserted-by":"publisher","DOI":"10.1093\/ajcp\/80.5.714"},{"key":"e_1_2_10_7_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJM199212033272303"},{"key":"e_1_2_10_8_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2672.2010.04679.x"},{"key":"e_1_2_10_9_1","doi-asserted-by":"publisher","DOI":"10.1086\/313890"},{"key":"e_1_2_10_10_1","doi-asserted-by":"publisher","DOI":"10.1111\/cmi.12070"},{"key":"e_1_2_10_11_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1462-5822.2011.01711.x"},{"key":"e_1_2_10_12_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0405284101"},{"key":"e_1_2_10_13_1","doi-asserted-by":"publisher","DOI":"10.1084\/jem.20040500"},{"key":"e_1_2_10_14_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1462-5822.2009.01313.x"},{"key":"e_1_2_10_15_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.0020115"},{"key":"e_1_2_10_16_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2958.2003.03964.x"},{"key":"e_1_2_10_17_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-020-17391-2"},{"key":"e_1_2_10_18_1","doi-asserted-by":"publisher","DOI":"10.1128\/msphere.00081-22"},{"key":"e_1_2_10_19_1","doi-asserted-by":"publisher","DOI":"10.1097\/DAD.0000000000001439"},{"key":"e_1_2_10_20_1","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.31.4.872-881.1993"},{"key":"e_1_2_10_21_1","doi-asserted-by":"publisher","DOI":"10.1097\/00006982-199803000-00015"},{"key":"e_1_2_10_22_1","doi-asserted-by":"publisher","DOI":"10.4269\/ajtmh.2010.09-0778"},{"key":"e_1_2_10_23_1","doi-asserted-by":"publisher","DOI":"10.3390\/tropicalmed4020069"},{"key":"e_1_2_10_24_1","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/btr039"},{"key":"e_1_2_10_25_1","first-page":"521","article-title":"Rochalimaea species stimulate human endothelial cell proliferation and migration in vitro","volume":"124","author":"Conley T","year":"1994","journal-title":"J Lab Clin Med"},{"key":"e_1_2_10_26_1","doi-asserted-by":"publisher","DOI":"10.1006\/mpat.1999.0315"},{"key":"e_1_2_10_27_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.actatropica.2015.11.019"},{"key":"e_1_2_10_28_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1000946"},{"key":"e_1_2_10_29_1","doi-asserted-by":"publisher","DOI":"10.1128\/iai.00186-22"},{"key":"e_1_2_10_30_1","doi-asserted-by":"publisher","DOI":"10.1002\/ece3.702"}],"container-title":["Microbiology and Immunology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/1348-0421.13057","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1111\/1348-0421.13057","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/1348-0421.13057","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,8,22]],"date-time":"2023-08-22T18:00:32Z","timestamp":1692727232000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/1348-0421.13057"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,3,15]]},"references-count":29,"journal-issue":{"issue":"5","published-print":{"date-parts":[[2023,5]]}},"alternative-id":["10.1111\/1348-0421.13057"],"URL":"https:\/\/doi.org\/10.1111\/1348-0421.13057","archive":["Portico"],"relation":{},"ISSN":["0385-5600","1348-0421"],"issn-type":[{"type":"print","value":"0385-5600"},{"type":"electronic","value":"1348-0421"}],"subject":[],"published":{"date-parts":[[2023,3,15]]},"assertion":[{"value":"2023-01-14","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2023-02-19","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2023-03-15","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}