{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,27]],"date-time":"2026-03-27T01:16:47Z","timestamp":1774574207006,"version":"3.50.1"},"reference-count":50,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2023,4,4]],"date-time":"2023-04-04T00:00:00Z","timestamp":1680566400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100003844","name":"Inamori Foundation","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100003844","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001691","name":"Japan Society for the Promotion of Science","doi-asserted-by":"publisher","award":["18K08004"],"award-info":[{"award-number":["18K08004"]}],"id":[{"id":"10.13039\/501100001691","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001691","name":"Japan Society for the Promotion of Science","doi-asserted-by":"publisher","award":["22K08579"],"award-info":[{"award-number":["22K08579"]}],"id":[{"id":"10.13039\/501100001691","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001691","name":"Japan Society for the Promotion of Science","doi-asserted-by":"publisher","award":["JSJSBP120188201"],"award-info":[{"award-number":["JSJSBP120188201"]}],"id":[{"id":"10.13039\/501100001691","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Microbiology and Immunology"],"published-print":{"date-parts":[[2023,6]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Hepatitis B virus (HBV) is a leading cause of chronic hepatitis, liver cirrhosis, and hepatocellular carcinoma. Current therapeutic drugs for chronic HBV infection use IFN and nucleos(t)ide analogs; however, their efficacy is limited. Thus, there is an urgent need to develop new antivirals for HBV therapy. In this study, we identified a plant\u2010derived polyphenolic bioflavonoid, amentoflavone, as a new anti\u2010HBV compound. Amentoflavone treatment dose\u2010dependently inhibited HBV infection in HBV\u2010susceptible cells with HepG2\u2010hNTCP\u2010C4 and primary human hepatocyte PXB\u2010cells. A mode\u2010of\u2010action study showed that amentoflavone inhibits the viral entry step, but not the viral internalization and early replication processes. Attachment of HBV particles as well as HBV preS1 peptide to HepG2\u2010hNTCP\u2010C4 cells was inhibited by amentoflavone. The transporter assay revealed that amentoflavone partly inhibits uptake of sodium taurocholate cotransporting polypeptide (NTCP)\u2013mediated bile acid. Furthermore, effect of various amentoflavone analogs on HBs and HBe production from HBV\u2010infected HepG2\u2010hNTCP\u2010C4 cells was examined. Robustaflavone exhibited comparable anti\u2010HBV activity to that of amentoflavone and an amentoflavone\u20107,4', 4\u2034\u2010trimethyl ether derivative (sciadopitysin) with moderate anti\u2010HBV activity. Cupressuflavone or the monomeric flavonoid apigenin did not exhibit the antiviral activity. Amentoflavone and its structurally related biflavonoids may provide a potential drug scaffold in the design of a new anti\u2010HBV drug inhibitor targeting NTCP.<\/jats:p>","DOI":"10.1111\/1348-0421.13064","type":"journal-article","created":{"date-parts":[[2023,3,17]],"date-time":"2023-03-17T05:45:10Z","timestamp":1679031910000},"page":"281-292","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":10,"title":["Amentoflavone inhibits hepatitis B virus infection via the suppression of preS1 binding to host cells"],"prefix":"10.1111","volume":"67","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-0466-5398","authenticated-orcid":false,"given":"Chie","family":"Aoki\u2010Utsubo","sequence":"first","affiliation":[{"name":"Department of Public Health Kobe University Graduate School of Health Sciences Kobe Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Puguh","family":"Indrasetiawan","sequence":"additional","affiliation":[{"name":"Department of Public Health Kobe University Graduate School of Health Sciences Kobe Japan"},{"name":"Division of Infectious Disease Pathology Kobe University Graduate School of Medicine Kobe Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0030-6072","authenticated-orcid":false,"given":"Kento","family":"Fukano","sequence":"additional","affiliation":[{"name":"Department of Virology II National Institute of Infectious Diseases Tokyo Japan"},{"name":"Center for Clinical Sciences National Center for Global Health and Medicine Tokyo Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0153-3533","authenticated-orcid":false,"given":"Masamichi","family":"Muramatsu","sequence":"additional","affiliation":[{"name":"Department of Virology II National Institute of Infectious Diseases Tokyo Japan"},{"name":"Department of Infectious Disease Research, Foundation for Biomedical Research and Innovation at Kobe Institute of Biomedical Research and Innovation Kobe Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nina","family":"Artanti","sequence":"additional","affiliation":[{"name":"Research Center for Chemistry, National Research and Innovation Agency (BRIN), Kawasan Puspiptek Serpong Indonesia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Muhammad","family":"Hanafi","sequence":"additional","affiliation":[{"name":"Research Center for Chemistry, National Research and Innovation Agency (BRIN), Kawasan Puspiptek Serpong Indonesia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hak","family":"Hotta","sequence":"additional","affiliation":[{"name":"Department of Public Health Kobe University Graduate School of Health Sciences Kobe Japan"},{"name":"Faculty of Clinical Nutrition and Dietetics Konan Women's University Kobe Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5525-9915","authenticated-orcid":false,"given":"Masanori","family":"Kameoka","sequence":"additional","affiliation":[{"name":"Department of Public Health Kobe University Graduate School of Health Sciences Kobe Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2023,4,4]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/S2468-1253(19)30119-0"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(16)30579-7"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrdp.2018.35"},{"key":"e_1_2_10_5_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12985-021-01589-x"},{"key":"e_1_2_10_6_1","doi-asserted-by":"publisher","DOI":"10.7554\/eLife.00049"},{"key":"e_1_2_10_7_1","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.73.3.2052-2057.1999"},{"key":"e_1_2_10_8_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1811064116"},{"key":"e_1_2_10_9_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12072-017-9829-7"},{"key":"e_1_2_10_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.antiviral.2020.104925"},{"key":"e_1_2_10_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jhep.2014.05.018"},{"key":"e_1_2_10_12_1","doi-asserted-by":"publisher","DOI":"10.1002\/hep.26982"},{"key":"e_1_2_10_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.antiviral.2012.12.008"},{"key":"e_1_2_10_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.antiviral.2014.09.009"},{"key":"e_1_2_10_15_1","doi-asserted-by":"publisher","DOI":"10.3851\/IMP2965"},{"key":"e_1_2_10_16_1","doi-asserted-by":"publisher","DOI":"10.1002\/hep.28952"},{"key":"e_1_2_10_17_1","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2018.03257"},{"key":"e_1_2_10_18_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-020-78618-2"},{"key":"e_1_2_10_19_1","doi-asserted-by":"publisher","DOI":"10.3390\/v13061169"},{"key":"e_1_2_10_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jhep.2018.03.026"},{"key":"e_1_2_10_21_1","doi-asserted-by":"publisher","DOI":"10.3390\/medicines6020066"},{"key":"e_1_2_10_22_1","doi-asserted-by":"publisher","DOI":"10.3389\/fphar.2021.768708"},{"key":"e_1_2_10_23_1","doi-asserted-by":"publisher","DOI":"10.3390\/plants11101381"},{"key":"e_1_2_10_24_1","doi-asserted-by":"publisher","DOI":"10.1248\/cpb.c12-01057"},{"key":"e_1_2_10_25_1","doi-asserted-by":"publisher","DOI":"10.3390\/molecules22020299"},{"key":"e_1_2_10_26_1","doi-asserted-by":"publisher","DOI":"10.1021\/np9700275"},{"key":"e_1_2_10_27_1","doi-asserted-by":"publisher","DOI":"10.1055\/s-1999-13971"},{"key":"e_1_2_10_28_1","doi-asserted-by":"publisher","DOI":"10.3390\/v11050466"},{"key":"e_1_2_10_29_1","first-page":"71","article-title":"Antiviral activity of cananga odorata against hepatitis b virus","volume":"65","author":"Indrasetiawan P","year":"2019","journal-title":"Kobe J Med Sci"},{"key":"e_1_2_10_30_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12250-015-3584-5"},{"key":"e_1_2_10_31_1","doi-asserted-by":"publisher","DOI":"10.1021\/acsomega.1c04320"},{"key":"e_1_2_10_32_1","doi-asserted-by":"publisher","DOI":"10.1007\/s13205-017-0599-7"},{"key":"e_1_2_10_33_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbrc.2013.12.052"},{"key":"e_1_2_10_34_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbrc.2014.08.029"},{"key":"e_1_2_10_35_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jhep.2016.11.009"},{"key":"e_1_2_10_36_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41586-022-04845-4"},{"key":"e_1_2_10_37_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jhep.2013.11.022"},{"key":"e_1_2_10_38_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00705-011-1164-z"},{"key":"e_1_2_10_39_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bmc.2010.09.035"},{"key":"e_1_2_10_40_1","doi-asserted-by":"publisher","DOI":"10.1142\/S0192415X18500441"},{"key":"e_1_2_10_41_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0166-3542(98)00033-3"},{"key":"e_1_2_10_42_1","doi-asserted-by":"publisher","DOI":"10.1053\/j.gastro.2005.03.090"},{"key":"e_1_2_10_43_1","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.79.3.1613-1622.2005"},{"key":"e_1_2_10_44_1","doi-asserted-by":"publisher","DOI":"10.1007\/s40265-020-01400-1"},{"key":"e_1_2_10_45_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-017-15338-0"},{"key":"e_1_2_10_46_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41426-018-0137-7"},{"key":"e_1_2_10_47_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M114.602540"},{"key":"e_1_2_10_48_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-021-98243-x"},{"key":"e_1_2_10_49_1","doi-asserted-by":"publisher","DOI":"10.1080\/10717544.2020.1716876"},{"key":"e_1_2_10_50_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.apsb.2021.05.014"},{"key":"e_1_2_10_51_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijpharm.2020.119560"}],"container-title":["Microbiology and Immunology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/1348-0421.13064","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/1348-0421.13064","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,8,20]],"date-time":"2023-08-20T12:05:53Z","timestamp":1692533153000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/1348-0421.13064"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,4,4]]},"references-count":50,"journal-issue":{"issue":"6","published-print":{"date-parts":[[2023,6]]}},"alternative-id":["10.1111\/1348-0421.13064"],"URL":"https:\/\/doi.org\/10.1111\/1348-0421.13064","archive":["Portico"],"relation":{},"ISSN":["0385-5600","1348-0421"],"issn-type":[{"value":"0385-5600","type":"print"},{"value":"1348-0421","type":"electronic"}],"subject":[],"published":{"date-parts":[[2023,4,4]]},"assertion":[{"value":"2022-12-19","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2023-03-13","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2023-04-04","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}