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We created lectin columns using high\u2010mannose (HM)\u2013type glycan\u2010specific lectins OAA and KAA\u20101 or core fucose\u2013specific lectin hypninA\u20102 and conducted binding experiments with SARS\u2010CoV\u20102. The results showed that these lectins were capable of binding to the virus. Furthermore, when examining the neutralization ability of nine different lectins, it was found that KAA\u20101, ESA\u20102, and hypninA\u20102 were effective in neutralizing SARS\u2010CoV\u20102. In competitive inhibition experiments with glycoproteins, neutralization was confirmed to occur through HM\u2010type or core fucose\u2013type glycans. However, neutralization was not observed with other lectins, such as OAA. This trend of KAA\u20101 and ESA\u20102 having the neutralizing ability and OAA not having it was also similar to influenza viruses. Electron microscopy observations revealed that KAA\u20101 and hypninA\u20102 strongly aggregated SARS\u2010CoV\u20102 particles, while OAA showed a low degree of aggregation. It is believed that the neutralization of SARS\u2010CoV\u20102 involves multiple factors, such as glycan attachment sites on the S protein, the size of lectins, and their propensity to aggregate, which cause inhibition of receptor binding or aggregation of virus particles. This study demonstrated that several algae\u2010derived lectins could neutralize SARS\u2010CoV\u20102 and that lectin columns can effectively recover and concentrate the virus.<\/jats:p>","DOI":"10.1111\/1348-0421.13082","type":"journal-article","created":{"date-parts":[[2023,5,30]],"date-time":"2023-05-30T01:19:53Z","timestamp":1685409593000},"page":"334-344","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":10,"title":["Capture and neutralization of SARS\u2010CoV\u20102 and influenza virus by algae\u2010derived lectins with high\u2010mannose and core fucose specificities"],"prefix":"10.1111","volume":"67","author":[{"given":"Tanuza","family":"Nazmul","sequence":"first","affiliation":[{"name":"Department of Virology, Graduate School of Biomedical and Health Sciences Hiroshima University Hiroshima 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