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Macrophages, major components of the innate immune system, can resist chlamydial infections and trigger adaptive immune responses. However, the molecular mechanisms underlying the action of macrophages against <jats:italic>C. psittaci<\/jats:italic> infection are not well understood. This study investigated the roles and mechanisms of plasmid\u2010encoded protein CPSIT_p7 of <jats:italic>C. psittaci<\/jats:italic> in regulating autophagy in RAW264.7 cells. The results demonstrated that stimulation of RAW264.7 with <jats:italic>C. psittaci<\/jats:italic> plasmid protein CPSIT_p7 induced the expressions of the autophagy signaling primary regulators LC3 and Beclin1, which could also significantly induce the phosphorylation levels of ERK, JNK, p38, and Akt. Next, siRNA knockdown of TLR2 resulted in significant downregulation of CPSIT_p7\u2010triggered autophagy in RAW264.7 cells. Moreover, the extracellular regulated protein kinase (ERK) inhibitor PD98059 markedly reduced autophagy in CPSIT_p7\u2010stimulated macrophages. In summary, these results indicated that TLR2 plays an essential role in the induction of autophagy through the ERK signaling pathway in CPSIT_p7\u2010stimulated RAW264.7 cells.<\/jats:p>","DOI":"10.1111\/1348-0421.13096","type":"journal-article","created":{"date-parts":[[2023,8,24]],"date-time":"2023-08-24T13:01:31Z","timestamp":1692882091000},"page":"469-479","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["TLR2 mediates autophagy through ERK signaling pathway in <i>Chlamydia psittaci<\/i> CPSIT_p7 protein\u2010stimulated RAW264.7 cells"],"prefix":"10.1111","volume":"67","author":[{"given":"Ying","family":"Luo","sequence":"first","affiliation":[{"name":"Institution of Pathogenic Biology, Hengyang Medical College University of South China Hengyang China"},{"name":"Department of Molecular Diagnosis Center The Sixth Affiliated Hospital of Guangzhou Medical University\/Qingyuan People's Hospital Qingyuan 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