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The recombinant bacillus Calmette Gu\u00e9rin (rBCG) vaccine, which expresses foreign antigens, is expected to be a superior vaccine against infectious diseases. Here, we report a new recombination platform in which the BCG Tokyo strain is transformed with nucleotide sequences encoding foreign protein fused with the MPB70 immunogenic protein precursor. By RNA\u2010sequencing, <jats:italic>mpb70<\/jats:italic> was found to be the most transcribed among all known genes of BCG Tokyo. Small oligopeptide, namely, polyhistidine tag, was able to be expressed in and secreted from rBCG through a process in which polyhistidine tag fused with intact MPB70 were transcribed by an <jats:italic>mpb70<\/jats:italic> promoter. This methodology was applied to develop an rBCG expressing the receptor binding domain (RBD) of severe acute respiratory syndrome coronavirus 2. Immunoblotting images and mass spectrometry data showed that RBD was also secreted from rBCG. Sera from mice vaccinated with the rBCG showed a tendency of weak neutralizing capacity. The secretion was retained even after a freeze\u2010drying process. The freeze\u2010dried rBCG was administered to and recovered from mice. Recovered rBCG kept secreting RBD. Collectively, our recombination platform offers stable secretion of foreign antigens and can be applied to the development of practical rBCGs.<\/jats:p>","DOI":"10.1111\/1348-0421.13116","type":"journal-article","created":{"date-parts":[[2024,1,31]],"date-time":"2024-01-31T13:28:45Z","timestamp":1706707725000},"page":"130-147","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Genetic engineering employing MPB70 and its promoter enables efficient secretion and expression of foreign antigen in bacillus Calmette Gu\u00e9rin (BCG) Tokyo"],"prefix":"10.1111","volume":"68","author":[{"given":"Atsuki","family":"Takeishi","sequence":"first","affiliation":[{"name":"Department of Bacteriology School of Medicine, Niigata University Niigata Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Amina K.","family":"Shaban","sequence":"additional","affiliation":[{"name":"Department of Bacteriology School of 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