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Upon pneumococcal infection, innate immune cells recognize pneumococcal lipoproteins via Toll\u2010like receptor 2\u00a0and induce inflammation. Here, we generated a strain of <jats:italic>S. pneumoniae<\/jats:italic> deficient in lipoprotein signal peptidase (LspA), a transmembrane type II signal peptidase required for lipoprotein maturation, to investigate the host immune response against this strain. Triton X\u2010114 phase separation revealed that lipoprotein expression was lower in the LspA\u2010deficient strain than in the wild\u2010type strain. Additionally, the LspA\u2010deficient strain decreased nuclear factor\u2010\u03baB activation and cytokine production in THP\u20101 cells, indicating impaired innate immune response against the strain.<\/jats:p>","DOI":"10.1111\/1348-0421.13117","type":"journal-article","created":{"date-parts":[[2024,2,7]],"date-time":"2024-02-07T06:58:38Z","timestamp":1707289118000},"page":"155-159","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Lipoprotein signal peptidase\u2010deficient <i>Streptococcus pneumoniae<\/i> exhibits impaired Toll\u2010like receptor 2\u2010stimulatory activity"],"prefix":"10.1111","volume":"68","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-2911-826X","authenticated-orcid":false,"given":"Hisanori","family":"Domon","sequence":"first","affiliation":[{"name":"Division of Microbiology and Infectious Diseases Niigata University Graduate School of Medical and Dental Sciences Niigata 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