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In the present study, we performed the long\u2010term cultivation of COS\u20107 cells transfected with archetype KOM\u20105 DNA, which were designated as COS\u2010BK cells. BKPyV derived from COS\u2010BK cells was characterized by analyzing the amount of the virus based on hemagglutination, viral replication, and the production of viral protein 1 (VP1). Immunostaining showed that VP1\u2010positive cells accounted for a small percentage of COS\u2010BK cells. The nucleotide sequences encompassing the origin of the DNA replication of BKPyV derived from COS\u2010BK cells were generated from KOM\u20105 by the deletion of an 8\u2010bp sequence, which did not involve T antigen binding sites. BKPyV replicated most efficiently in COS\u2010BK cells in DMEM containing 2% fetal bovine serum. These results indicate that COS\u2010BK cells are a suitable culture system for studying the persistent infection of archetype BKPyV.<\/jats:p>","DOI":"10.1111\/1348-0421.13124","type":"journal-article","created":{"date-parts":[[2024,3,4]],"date-time":"2024-03-04T05:28:37Z","timestamp":1709530117000},"page":"179-184","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Establishment of COS\u2010BK cells persistently infected with archetype BK polyomavirus"],"prefix":"10.1111","volume":"68","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-4199-0090","authenticated-orcid":false,"given":"Souichi","family":"Nukuzuma","sequence":"first","affiliation":[{"name":"Research Laboratory KinoPharma Inc. 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Kyoto Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tetsuro","family":"Suzuki","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Immunology Hamamatsu University School of Medicine Hamamatsu Shizuoka Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2024,3,3]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(71)91776-4"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1086\/431488"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1002\/jmv.1890410408"},{"key":"e_1_2_9_5_1","first-page":"94","article-title":"Pathogenicity of BK virus on the urinary system","volume":"73","author":"Krajewski W","year":"2020","journal-title":"Cent European J Urol"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.virol.2011.10.026"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1099\/vir.0.81698-0"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/0092-8674(81)90282-8"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00239-005-0092-5"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.7883\/yoken.JJID.2020.325"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.1111\/voxs.12294"},{"key":"e_1_2_9_12_1","doi-asserted-by":"publisher","DOI":"10.1099\/vir.0.80363-0"},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.1128\/MRA.01129-19"},{"key":"e_1_2_9_14_1","doi-asserted-by":"publisher","DOI":"10.1128\/mBio.02356-21"},{"key":"e_1_2_9_15_1","doi-asserted-by":"publisher","DOI":"10.1136\/bmj.283.6303.1363-a"},{"key":"e_1_2_9_16_1","doi-asserted-by":"publisher","DOI":"10.1128\/jvi.61.5.1747-1750.1987"},{"key":"e_1_2_9_17_1","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.00542-09"},{"key":"e_1_2_9_18_1","doi-asserted-by":"publisher","DOI":"10.1002\/jmv.1890430104"},{"key":"e_1_2_9_19_1","doi-asserted-by":"publisher","DOI":"10.1002\/jmv.1890470413"}],"container-title":["Microbiology and Immunology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/1348-0421.13124","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,5,7]],"date-time":"2024-05-07T04:05:08Z","timestamp":1715054708000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/1348-0421.13124"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,3,3]]},"references-count":18,"journal-issue":{"issue":"5","published-print":{"date-parts":[[2024,5]]}},"alternative-id":["10.1111\/1348-0421.13124"],"URL":"https:\/\/doi.org\/10.1111\/1348-0421.13124","archive":["Portico"],"relation":{},"ISSN":["0385-5600","1348-0421"],"issn-type":[{"type":"print","value":"0385-5600"},{"type":"electronic","value":"1348-0421"}],"subject":[],"published":{"date-parts":[[2024,3,3]]},"assertion":[{"value":"2024-01-17","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2024-02-17","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2024-03-03","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}