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Enterococcal isolates were collected from inpatients at Hiroshima University Hospital from April 1\u00a0to June 30, 2021 using selective medium for Enterococci. Multilocus sequence typing, antimicrobial susceptibility testing, and whole\u2010genome sequencing were performed. A total of 164 isolates, including\u00a0<jats:italic>Enterococcus faecium<\/jats:italic> (41 isolates), <jats:italic>Enterococcus faecalis<\/jats:italic> (80 isolates), <jats:italic>Enterococcus raffinosus<\/jats:italic> (11 isolates), <jats:italic>Enterococcus casseliflavus<\/jats:italic> (nine isolates), <jats:italic>Enterococcus avium<\/jats:italic> (12 isolates), <jats:italic>Enterococcus lactis<\/jats:italic> (eight isolates), <jats:italic>Enterococcus gallinarum<\/jats:italic> (two isolates), and <jats:italic>Enterococcus malodoratus<\/jats:italic> (one isolate), were analyzed. We found one <jats:italic>vanA<\/jats:italic>\u2010positive <jats:italic>E. faecium<\/jats:italic>, which was already informed when the patient was transferred to the hospital, nine\u00a0<jats:italic>vanC<\/jats:italic>\u2010positive <jats:italic>E. casseliflavus<\/jats:italic>, and two\u00a0<jats:italic>vanC<\/jats:italic>\u2010positive <jats:italic>E. gallinarum. E. faecium<\/jats:italic> isolates showed resistance to ampicillin (95.1%), imipenem (95.1%), and levofloxacin (87.8%), and <jats:italic>E. faecalis<\/jats:italic> isolates showed resistance to minocycline (49.4%). Ampicillin\u2010 and levofloxacin\u2010resistant <jats:italic>E. faecium<\/jats:italic> had multiple mutations in penicillin\u2010binding protein 5 (PBP5) (39\/39 isolates) and ParC\/GyrA (21\/36 isolates), respectively. <jats:italic>E. raffinosus<\/jats:italic> showed resistance to ampicillin (81.8%), imipenem (45.5%), and levofloxacin (45.5%), and <jats:italic>E. lactis<\/jats:italic> showed resistance to ampicillin (37.5%) and imipenem (50.0%). The linezolid resistance genes <jats:italic>optrA<\/jats:italic> and <jats:italic>cfr<\/jats:italic>(B) were found only in one isolate of <jats:italic>E. faecalis<\/jats:italic> and <jats:italic>E. raffinosus<\/jats:italic>, respectively. This study, showing the status of enterococci infection in hospitalized patients, is one of the important information when considering nosocomial infection control of VRE.<\/jats:p>","DOI":"10.1111\/1348-0421.13155","type":"journal-article","created":{"date-parts":[[2024,6,14]],"date-time":"2024-06-14T09:59:57Z","timestamp":1718359197000},"page":"254-266","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":15,"title":["Antibiotic susceptibility and genome analysis of <i>Enterococcus<\/i> species isolated from inpatients in one hospital with no apparent outbreak of vancomycin\u2010resistant <i>Enterococcus<\/i> in Japan"],"prefix":"10.1111","volume":"68","author":[{"given":"Ayumi","family":"Fujii","sequence":"first","affiliation":[{"name":"Department of Oral and Maxillofacial Surgery Hiroshima University Graduate School of Biomedical and Health Sciences Hiroshima Japan"},{"name":"Department of Bacteriology Hiroshima University 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