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Here, we investigate the effects of EGCG on thymic involution induced by lipopolysaccharide (LPS), an endotoxin derived from Gram\u2010negative bacteria. The methodology included an in vivo experiment on female Kunming mice exposed to LPS and EGCG. Morphological assessment of thymic involution, immunohistochemical detection, and thymocyte subsets analysis by flow cytometry were further carried out to evaluate the potential role of EGCG on the thymus. As a result, we found that EGCG alleviated LPS\u2010induced thymic atrophy, increased mitochondrial membrane potential and superoxide dismutase levels, and decreased malondialdehyde and reactive oxygen species levels. In addition, EGCG pre\u2010supplement restored the ratio of thymocyte subsets, the expression of autoimmune regulator, sex\u2010determining region Y\u2010box 2,\u00a0and Nanog homebox, and reduced the number of senescent cells and collagen fiber deposition. Western blotting results indicated that EGCG treatment elevated LPS\u2010induced decrease in pAMPK, Sirt1 protein expression. Collectively, EGCG relieved thymus architecture and function damaged by LPS via regulation of AMPK\/Sirt1 signaling pathway. Our findings may provide a new strategy on protection of thymus from involution caused by LPS by using EGCG. And EGCG might be considered as a potential agent for the prevention and treatment of thymic involution.<\/jats:p>","DOI":"10.1111\/1348-0421.13159","type":"journal-article","created":{"date-parts":[[2024,6,17]],"date-time":"2024-06-17T23:16:25Z","timestamp":1718666185000},"page":"281-293","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["Epigallocatechin\u20103\u2010gallate ameliorates lipopolysaccharide\u2010induced acute thymus involution in mice via AMPK\/Sirt1 pathway"],"prefix":"10.1111","volume":"68","author":[{"given":"Qing","family":"Su","sequence":"first","affiliation":[{"name":"Department of Histology and Embryology, School of Basic Medical Sciences Zhengzhou University Zhengzhou Henan PR China"},{"name":"The Second Affiliated Hospital of Zhengzhou University Zhengzhou Henan PR 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