{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,21]],"date-time":"2026-05-21T03:22:10Z","timestamp":1779333730168,"version":"3.51.4"},"reference-count":38,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2025,4,1]],"date-time":"2025-04-01T00:00:00Z","timestamp":1743465600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Microbiology and Immunology"],"published-print":{"date-parts":[[2025,6]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:p>Silicosis is a lung disease that is very harmful. This makes the disease worse. This study looks at how the fat mass and obesity associated (FTO) gene affects macrophage M1\/M2 polarisation and pulmonary fibrosis in silicosis. Macrophages were isolated from alveolar lavage fluid in silicosis and bronchiectasis (BE) patients. Gene expression was detected by reverse transcription\u2010PCR (RT\u2010PCR). Pulmonary fibrosis was assessed by CT<jats:sub>FLV\/TLV%<\/jats:sub> using 3D CT and Masson staining assay. Enzyme\u2010linked immunosorbent assay was applied to assess inflammatory factor level. The macrophage M1\/M2 polarization characteristics (iNOS, CD206) was quantified by Immunofluorescence and Flow cytometry assays. Silicosis patients alveolar lavage macrophages polarized towards M1 type, and the expression level of M1 polarization\u2010related chemokines also increased. More importantly, FTO gene downregulation promotes macrophage polarization to M1 type and the secretion of pro\u2010inflammatory factor TNF\u2010\u03b1 and IL\u20106. And FTO knockdown can strengthen the glycolysis of macrophages, especially anaerobic glycolysis, thus inducing macrophages M1 polarization. Moreover, downregulation of FTO ameliorates silicosis pulmonary fibrosis. And FTO upregulation is associated with the M2 polarization of macrophage and the deterioration of pulmonary fibrosis in silicosis patients. FTO downregulation facilitates the infiltration of inflammatory cells by promoting M1 polarization of macrophages in silicosis.<\/jats:p>","DOI":"10.1111\/1348-0421.13216","type":"journal-article","created":{"date-parts":[[2025,4,2]],"date-time":"2025-04-02T12:36:49Z","timestamp":1743597409000},"page":"339-349","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["FTO Aggravates the Infiltration of Inflammatory Cells and Pulmonary Fibrosis in Silicosis Though Inducing the Imbalance of Macrophages Polarization"],"prefix":"10.1111","volume":"69","author":[{"given":"Jian\u2010min","family":"Fan","sequence":"first","affiliation":[{"name":"Department of Occupational Diseases Affiliated Maternity and Child Health Care Hospital of Nantong University Nantong Jiangsu China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0009-0001-1222-1439","authenticated-orcid":false,"given":"Xu","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of emergency internal medicine Affiliated Maternity and Child Health Care Hospital of Nantong University Nantong Jiangsu China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2025,4]]},"reference":[{"key":"e_1_2_13_2_1","doi-asserted-by":"crossref","first-page":"789","DOI":"10.3348\/jksr.2023.0128","article-title":"Caplan Syndrome Mimicking Progressive Massive Fibrosis on CT: A Case Report","volume":"85","author":"Jinwook B.","year":"2024","journal-title":"Journal of the Korean Society of Radiology"},{"key":"e_1_2_13_3_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12889-022-13541-x"},{"key":"e_1_2_13_4_1","doi-asserted-by":"crossref","first-page":"903","DOI":"10.1097\/JOM.0000000000003201","article-title":"Neuron\u2010Specific Enolase (NSE) as a Biological Marker for Welders' Pneumoconiosis","volume":"66","author":"Ghada Hossam El\u2010Din E.\u2010S.","year":"2024","journal-title":"Journal of Occupational and Environmental Medicine"},{"key":"e_1_2_13_5_1","doi-asserted-by":"crossref","DOI":"10.1016\/j.biopha.2024.117216","article-title":"Mefunidone Alleviates Silica\u2010Induced Inflammation and Fibrosis by Inhibiting the TLR4\u2010NF\u2010\u03baB\/MAPK Pathway and Attenuating Pyroptosis in Murine Macrophages","volume":"178","author":"Lingzhi L.","year":"2024","journal-title":"Biomedicine & Pharmacotherapy"},{"key":"e_1_2_13_6_1","first-page":"530","article-title":"[Analysis of Clinical Characteristics and Causes of Death in 38 Patients With Pneumoconiosis]","volume":"42","author":"Jing H.","year":"2024","journal-title":"Zhonghua Lao Dong Wei Sheng Zhi Ye Bing Za Zhi"},{"key":"e_1_2_13_7_1","first-page":"523","article-title":"[Comparative Study on CT Image Characteristics of Pneumoconiosis Large Shadow and Primary Lung Cancer Mass]","volume":"42","author":"Wang C. 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