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Most <jats:italic>Lactobacillus<\/jats:italic> species play a crucial role in not only inhibiting BV, but also maintaining vaginal health. We aimed to determine whether <jats:italic>Lactobacillus plantarum<\/jats:italic> ATG\u2010K2 has therapeutic and preventive effects against <jats:italic>Gardnerella vaginalis<\/jats:italic> (GV), a representative pathogen of BV. In vitro cell assays confirmed that ATG\u2010K2 clusters within cells without cytotoxic effects and inhibits both the cytotoxicity and GV adhesion. Furthermore, biofilm formation assays using crystal violet revealed that ATG\u2010K2 disrupts GV\u2010formed biofilms. In GV\u2010induced BV mouse models, ATG\u2010K2 reduced clue cell formation and sialidase activity, thereby addressing the key aspects of BV. Additionally, when ATG\u2010K2 was administered, the measurement of GV DNA within the vaginal tissue revealed a reduction in GV. In conclusion, ATG\u2010K2 holds promise as a therapeutic agent for BV by inhibiting GV colonization and the associated pathology, potentially offering an alternative to antibiotics.<\/jats:p>","DOI":"10.1111\/1348-0421.70001","type":"journal-article","created":{"date-parts":[[2025,7,18]],"date-time":"2025-07-18T09:14:14Z","timestamp":1752830054000},"page":"457-465","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Therapeutic Potential of <i>Lactobacillus plantarum<\/i> ATG\u2010K2 in Bacterial Vaginosis: In Vitro and In Vivo Evidence"],"prefix":"10.1111","volume":"69","author":[{"ORCID":"https:\/\/orcid.org\/0009-0000-7673-2441","authenticated-orcid":false,"given":"Yeon\u2010Hwa","family":"Park","sequence":"first","affiliation":[{"name":"AtoGen Co. 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