{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,2]],"date-time":"2025-11-02T06:10:51Z","timestamp":1762063851994,"version":"build-2065373602"},"reference-count":39,"publisher":"Wiley","issue":"11","license":[{"start":{"date-parts":[[2025,9,30]],"date-time":"2025-09-30T00:00:00Z","timestamp":1759190400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Microbiology and Immunology"],"published-print":{"date-parts":[[2025,11]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Surveillance of SARS\u2010CoV\u20102 in white\u2010tailed deer (WTD,\n                    <jats:italic>Odocoileus virginianus<\/jats:italic>\n                    ) has revealed its widespread and sustained transmission across North America, with evidence suggesting possible transmission from deer to humans. In the following surveillance studies in other deer species, however, little evidence of infection spread was found, including in sika deer (\n                    <jats:italic>Cervus nippon<\/jats:italic>\n                    ) in our previous study. Differences in the structure of the virus entry receptor angiotensin\u2010converting enzyme 2 (ACE2) are known to act as one of the functional barriers to SARS\u2010CoV\u20102 infection. To investigate the molecular basis of the lack of SARS\u2010CoV\u20102 transmission to sika deer, we performed structural and functional analyses of the sika deer ACE2 in comparison with WTD ACE2. Comparison of sika deer ACE2 sequence and those of cervid species with WTD ACE2, followed by in silico molecular dynamics analysis, revealed a substitution of lysine to asparagine in position 31 commonly found in cervid ACE2s can potentially alter binding to the SARS\u2010CoV\u20102 spike (S) protein receptor\u2010binding domain (RBD). Functional assays in cells expressing sika deer and WTD ACE2s showed minimal differences in viral binding and replication, demonstrating that SARS\u2010CoV\u20102 can similarly utilize ACE2 from both species. These findings suggest that sika deer and possibly other cervids may be highly susceptible to SARS\u2010CoV\u20102 and highlight the need to investigate other factors impacting virus spread in deer populations.\n                  <\/jats:p>","DOI":"10.1111\/1348-0421.70012","type":"journal-article","created":{"date-parts":[[2025,9,30]],"date-time":"2025-09-30T13:50:17Z","timestamp":1759240217000},"page":"533-543","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Molecular and Functional Characterization of Sika Deer ACE2 as a Receptor for SARS\u2010CoV\u20102"],"prefix":"10.1111","volume":"69","author":[{"given":"Anastasiia","family":"Kovba","sequence":"first","affiliation":[{"name":"Graduate School of Veterinary Medicine Hokkaido University Sapporo City Hokkaido Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Manabu","family":"Igarashi","sequence":"additional","affiliation":[{"name":"Division 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