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While the circular polymerase extension reaction (CPER) has been applied to RNA viruses, it has not been used for DNA viruses. Here, we successfully applied CPER to generate infectious recombinant adenoviruses (AdVs) of two serotypes. The resulting viruses replicated comparably to parental strains, and replication was confirmed by immunostaining. These findings demonstrate that CPER is a rapid and efficient platform for reverse genetics of DNA viruses and may accelerate AdV research and therapeutic development.<\/jats:p>","DOI":"10.1111\/1348-0421.70016","type":"journal-article","created":{"date-parts":[[2025,10,13]],"date-time":"2025-10-13T06:29:21Z","timestamp":1760336961000},"page":"619-622","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["First Versatile Reverse Genetics System for DNA Viruses Using Circular Polymerase Extension Reaction"],"prefix":"10.1111","volume":"69","author":[{"given":"Hirotaka","family":"Yamamoto","sequence":"first","affiliation":[{"name":"Department of Microbiology and Immunology, Faculty of Medicine Hokkaido University Sapporo 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