{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,4]],"date-time":"2026-02-04T17:36:36Z","timestamp":1770226596206,"version":"3.49.0"},"reference-count":31,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2025,12,13]],"date-time":"2025-12-13T00:00:00Z","timestamp":1765584000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2025,12,13]],"date-time":"2025-12-13T00:00:00Z","timestamp":1765584000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Microbiology and Immunology"],"published-print":{"date-parts":[[2026,2]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:label\/>\n                    <jats:p>Psoriasis is a common immune\u2010mediated skin disorder. miR\u201034a, as a significant regulatory component, has been involved in the regulation of immune\u2010inflammatory responses. Previous studies have shown that miR\u201034a is abnormally expressed in psoriasis cell models. However, the role of miR\u201034a in psoriasis remains unvalidated by any existing studies. We co\u2010stimulated HaCaT cells with IL\u201017A, IL\u201022, TNF\u2010\u03b1, IL\u20101\u03b1, and M5 to establish an in vitro model of psoriasis. qPCR was used to detect the expression of miR\u201034a; ELISA was performed to measure the secretion levels of pro\u2010inflammatory cytokines IL\u20101\u03b2, IL\u20106, IL\u201017, and IL\u201023 in HaCaT cells; CCK\u20108 and EdU staining assays were used to assess cell growth and proliferation; Western blot analysis was employed to assess the expression levels of relevant proteins, including SIRT1, NLRP3, ASC, pro\u2010Caspase\u20101, and Caspase\u20101; dual\u2010luciferase reporter gene assays and RNA immunoprecipitation (RIP) were conducted to investigate the interaction between miR\u201034a and SIRT1. Additionally, a mouse psoriasis model was induced by imiquimod (IMQ) to validate the role of miR\u201034a in vivo. qPCR results found that miR\u201034a was upregulated in HaCaT cells. ELISA results indicated that the levels of IL\u20101\u03b2, IL\u20106, IL\u201017, and IL\u201023 were significantly elevated in the M5\u2010treated group. CCK\u20108 and EdU staining assays revealed that M5 treatment notably increased the proliferation and viability of HaCaT cells. After treatment with the miR\u201034a inhibitor, the levels of cytokines and cell proliferation were significantly reduced. Western blot analysis showed that M5 treatment resulted in a significant decrease in SIRT1 protein levels, while the expression levels of NLRP3, ASC, pro\u2010Caspase\u20101, and Caspase\u20101 were increased. In contrast, miR\u201034a\u2010inhibitor treatment showed the opposite results. Dual\u2010luciferase reporter and RIP assays indicated a negative feedback interaction between miR\u201034a and SIRT1. Rescue experiments showed that overexpression of SIRT1 effectively inhibited NLRP3 expression, significantly reduced IL\u201023\/IL\u201017 immune\u2010inflammatory responses, and suppressed HaCaT cell proliferation, while overexpression of NLRP3 had the opposite effects. In the IMQ\u2010induced mouse psoriasis model, the IMQ group showed severe psoriasis\u2010like phenotypes, obvious skin pathological changes, upregulated miR\u201034a expression, and increased skin cytokine levels; all these abnormalities were alleviated by miR\u201034a inhibitor treatment. miR\u201034a mediates the IL\u201023\/IL\u201017 immune\u2010inflammatory response and promotes HaCaT cell proliferation by regulating the SIRT1\/NLRP3 pathway.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/1348-0421.70032","type":"journal-article","created":{"date-parts":[[2025,12,13]],"date-time":"2025-12-13T16:31:01Z","timestamp":1765643461000},"page":"91-102","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["miR\u201034a Mediates IL\u201023\/IL\u201017 Immune Inflammation and Promotes Cell Proliferation in HaCaT Cells by Targeting SIRT1\/NLRP3"],"prefix":"10.1111","volume":"70","author":[{"given":"Guoxiang","family":"Qin","sequence":"first","affiliation":[{"name":"Department of Dermatology The First Affiliated Hospital of Guangxi University of Chinese Medicine Nanning People's Republic of China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zhihong","family":"Wu","sequence":"additional","affiliation":[{"name":"Department of Dermatology The First Affiliated Hospital of Guangxi University of Chinese Medicine Nanning People's Republic of China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ying","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Dermatology The First Affiliated Hospital of Guangxi University of Chinese Medicine Nanning People's Republic of China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Min","family":"Jia","sequence":"additional","affiliation":[{"name":"Department of Dermatology The First Affiliated Hospital of Guangxi University of Chinese Medicine Nanning People's Republic of China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yi","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Dermatology The First Affiliated Hospital of Guangxi University of Chinese Medicine Nanning People's Republic of China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xiaoyu","family":"Sun","sequence":"additional","affiliation":[{"name":"Department of Dermatology The First Affiliated Hospital of Guangxi University of Chinese Medicine Nanning People's Republic of China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xue","family":"Huang","sequence":"additional","affiliation":[{"name":"Department of Dermatology The First Affiliated Hospital of Guangxi University of Chinese Medicine Nanning People's Republic of China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tao","family":"Huang","sequence":"additional","affiliation":[{"name":"Department of Dermatology The First Affiliated Hospital of Guangxi University of Chinese Medicine Nanning People's Republic of China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0009-0004-1501-5157","authenticated-orcid":false,"given":"Jiang","family":"Zhong","sequence":"additional","affiliation":[{"name":"Department of Dermatology The First Affiliated Hospital of Guangxi University of Chinese Medicine Nanning People's Republic of China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2025,12,13]]},"reference":[{"key":"e_1_2_13_2_1","doi-asserted-by":"publisher","DOI":"10.2340\/00015555-3076"},{"key":"e_1_2_13_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.medmic.2020.100016"},{"key":"e_1_2_13_4_1","first-page":"99","article-title":"Psoriasis 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