{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,8]],"date-time":"2026-03-08T20:06:02Z","timestamp":1773000362614,"version":"3.50.1"},"reference-count":28,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2026,1,19]],"date-time":"2026-01-19T00:00:00Z","timestamp":1768780800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,1,19]],"date-time":"2026-01-19T00:00:00Z","timestamp":1768780800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Microbiology and Immunology"],"published-print":{"date-parts":[[2026,3]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Among the three prion strains of bovine spongiform encephalopathy (BSE), classical BSE (C\u2010BSE) prions are known causative agents of variant Creutzfeldt\u2013Jakob disease. By contrast, human infections with L\u2010type (L\u2010) or H\u2010type (H\u2010) BSE prions have not been reported. Nonetheless, the zoonotic potential of L\u2010BSE prions is supported by their successful primary transmission from cattle to cynomolgus macaque (\n                    <jats:italic>Macaca fascicularis<\/jats:italic>\n                    ) monkeys via intracranial challenge. To assess whether the defining strain traits of L\u2010BSE prions remain stable following secondary intraspecies transmission, we prepared brain homogenates from a diseased macaque that had previously undergone primary transmission of L\u2010BSE prions, and intracranially administered them into two na\u00efve macaques. Both animals succumbed to the disease within humane endpoints comparable to those observed in the primary transmission. Histopathological and immunohistochemical analyses of brain tissues showed no significant changes relative to primary transmission, including severe vacuolation and fine synaptic distribution of disease\u2010associated forms of prion protein (PrP\n                    <jats:sup>Sc<\/jats:sup>\n                    ) in the cerebrum, and sparse PrP\n                    <jats:sup>Sc<\/jats:sup>\n                    plaques in the cerebellum. In bioassays using C57BL\/6\u2009J mice, cattle\u2010derived L\u2010BSE prions and those passaged once or twice in macaques failed to transmit to mice, whereas cattle\u2010derived C\u2010BSE prions and their macaque\u2010passaged counterparts were transmissible. These findings refine our understanding of L\u2010BSE pathogenesis and confirm the stability of L\u2010BSE prions following intracranial transmission in a nonhuman primate model.\n                  <\/jats:p>","DOI":"10.1111\/1348-0421.70040","type":"journal-article","created":{"date-parts":[[2026,1,20]],"date-time":"2026-01-20T07:11:22Z","timestamp":1768893082000},"page":"166-174","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Strain Traits of Intracranially Administered L\u2010Type Bovine Spongiform Encephalopathy Prions Are not Significantly Modified During Intraspecies Transmission in Cynomolgus Monkeys"],"prefix":"10.1111","volume":"70","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-1948-6034","authenticated-orcid":false,"given":"Ken'ichi","family":"Hagiwara","sequence":"first","affiliation":[{"name":"Department of Biochemistry and Cell Biology National Institute of Infectious Diseases Tokyo Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0009-0009-8766-809X","authenticated-orcid":false,"given":"Hiroaki","family":"Shibata","sequence":"additional","affiliation":[{"name":"Tsukuba Primate Research Center, National Institutes of Biomedical Innovation Health and Nutrition Ibaraki Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Minoru","family":"Tobiume","sequence":"additional","affiliation":[{"name":"Department of Pathology National Institute of Infectious Diseases Tokyo Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2927-1149","authenticated-orcid":false,"given":"Yuko","family":"Sato","sequence":"additional","affiliation":[{"name":"Department of Pathology National Institute of Infectious Diseases Tokyo Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Keiko","family":"Ohto","sequence":"additional","affiliation":[{"name":"The Corporation for Production and Research of Laboratory Primates Ibaraki Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sachi","family":"Okabayashi","sequence":"additional","affiliation":[{"name":"The Corporation for Production and Research of Laboratory Primates Ibaraki Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nozomi","family":"Nakano","sequence":"additional","affiliation":[{"name":"The Corporation for Production and Research of Laboratory Primates Ibaraki Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1785-4355","authenticated-orcid":false,"given":"Motohiro","family":"Horiuchi","sequence":"additional","affiliation":[{"name":"Laboratory of Veterinary Hygiene, Faculty of Veterinary Medicine, Graduate School of Infectious Diseases Hokkaido University Hokkaido Japan"},{"name":"One Health Research Center Hokkaido University Hokkaido Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Fumiko","family":"Ono","sequence":"additional","affiliation":[{"name":"Department of Veterinary Associated Science, Faculty of Veterinary Medicine Okayama University of Science Okayama Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2026,1,19]]},"reference":[{"key":"e_1_2_11_2_1","volume-title":"Prion Biology and Disease","author":"Prusiner S. 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