{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,8]],"date-time":"2026-09-08T11:12:26Z","timestamp":1788865946145,"version":"build-2803163510"},"reference-count":33,"publisher":"Wiley","issue":"9","license":[{"start":{"date-parts":[[2026,7,1]],"date-time":"2026-07-01T00:00:00Z","timestamp":1782864000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,7,1]],"date-time":"2026-07-01T00:00:00Z","timestamp":1782864000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100001691","name":"Japan Society for the Promotion of Science","doi-asserted-by":"publisher","award":["25K02490"],"award-info":[{"award-number":["25K02490"]}],"id":[{"id":"10.13039\/501100001691","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Microbiology and Immunology"],"published-print":{"date-parts":[[2026,9]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    <jats:italic>Chlamydia trachomatis<\/jats:italic>\n                    (Ct) is an obligate intracellular bacterium that can cause severe reproductive complications, including infertility and pelvic inflammatory disease. Its pathogenicity depends on intracellular maturation, which involves differentiation between infectious elementary (EB) and replicative reticulate bodies within inclusion vacuoles. Ct is known to modulate host PI3K\u2010AKT signaling during this process; however, the molecular basis of this regulation remains unclear. To elucidate this mechanism, we screened host factors linked to the PI3K\u2010AKT axis using a PI3K\u2010AKT\u2010mTOR compound library comprising 319 inhibitors, and identified the adaptor protein Gab2 (GRB2\u2010associated binding protein 2) as a new target molecule of Ct. Gab2 protein levels decreased during the late phase of infection, and Gab2 silencing impaired intracellular replication without affecting EB formation. These findings demonstrate that Ct infection modulates the host adaptor Gab2 during intracellular development and provide new insights into host\u2013pathogen interactions underlying chlamydial maturation.\n                  <\/jats:p>","DOI":"10.1111\/1348-0421.70064","type":"journal-article","created":{"date-parts":[[2026,7,2]],"date-time":"2026-07-02T04:38:09Z","timestamp":1782967089000},"page":"393-401","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Infection\u2010Dependent Modulation of the Host Adaptor Gab2 During\n                    <i>Chlamydia trachomatis<\/i>\n                    Intracellular Development"],"prefix":"10.1111","volume":"70","author":[{"given":"Sora","family":"Kuroiwa","sequence":"first","affiliation":[{"name":"Department of Medical Laboratory Science, Faculty of Health Sciences Hokkaido University Sapporo Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Taiki","family":"Deguchi","sequence":"additional","affiliation":[{"name":"Department of Medical Laboratory Science, Faculty of Health Sciences Hokkaido University Sapporo Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Torahiko","family":"Okubo","sequence":"additional","affiliation":[{"name":"Department of Medical Laboratory Science, Faculty of Health Sciences Hokkaido University Sapporo Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shinji","family":"Nakamura","sequence":"additional","affiliation":[{"name":"Division of Biomedical Imaging Research Juntendo University Graduate School of Medicine, Bunkyo\u2010ku Tokyo Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hideaki","family":"Higashi","sequence":"additional","affiliation":[{"name":"Division of Infection and Immunity, International Institute for Zoonosis Control Hokkaido University Sapporo Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3723-3361","authenticated-orcid":false,"given":"Hiroyuki","family":"Yamaguchi","sequence":"additional","affiliation":[{"name":"Department of Medical Laboratory Science, Faculty of Health Sciences Hokkaido University Sapporo Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,7,1]]},"reference":[{"key":"e_1_2_11_2_1","first-page":"2779","article-title":"Gab2 Is a Novel Prognostic Factor for Colorectal Cancer Patients","volume":"8","author":"Ding C.","year":"2015","journal-title":"International Journal of Clinical and Experimental Pathology"},{"key":"e_1_2_11_3_1","volume-title":"Chlamydia: Intracellular Biology, Pathogenesis, and Immunity","author":"Schachter J.","year":"1999"},{"key":"e_1_2_11_4_1","volume-title":"Report on global sexually transmitted infection surveillance 2021","author":"World Health Organization","year":"2021"},{"key":"e_1_2_11_5_1","doi-asserted-by":"publisher","DOI":"10.1038\/nri1551"},{"issue":"2","key":"e_1_2_11_6_1","first-page":"423","article-title":"Pathogenesis of Chlamydia trachomatis Infection and Disease","volume":"23","author":"Haggerty C. 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