{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,7]],"date-time":"2026-08-07T14:34:06Z","timestamp":1786113246229,"version":"build-2736575974"},"reference-count":23,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2026,6,29]],"date-time":"2026-06-29T00:00:00Z","timestamp":1782691200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,6,29]],"date-time":"2026-06-29T00:00:00Z","timestamp":1782691200000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Microbiology and Immunology"],"published-print":{"date-parts":[[2026,8]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:label\/>\n                    <jats:p>\n                      Acne vulgaris is a chronic inflammatory disease of the pilosebaceous unit driven by\n                      <jats:italic>Cutibacterium acnes<\/jats:italic>\n                      colonization and the ensuing inflammatory response. Neutrophil extracellular traps (NETs) play a critical role in acne inflammation. Pipa Qingfei Decoction (PPQFY), a classic Traditional Chinese Medicine formula for acne, exhibits antibacterial and anti\u2010inflammatory effects, but whether it regulates NETs to alleviate\n                      <jats:italic>Cutibacterium<\/jats:italic>\n                      \u2010induced acne remains unclear. In vitro (keratinocytes\/neutrophils) and in vivo (C57BL\/6 mice) acne models were established using\n                      <jats:italic>Cutibacterium acnes<\/jats:italic>\n                      and treated with PPQFY, DNase I, or GSK484 (NET inhibitors). Neutrophil purity and Ly6G\n                      <jats:sup>+<\/jats:sup>\n                      CD11b\n                      <jats:sup>+<\/jats:sup>\n                      cell ratio were assessed by flow cytometry. Cell viability and proliferation were evaluated using CCK\u20108 and EdU assays. Levels of IL\u20101\u03b2, TNF\u2010\u03b1, and MPO\u2010DNA were measured by ELISA. Protein levels of PADI4, CitH3, and MPO were determined by western blotting. Skin pathological changes were examined by H&amp;E staining. PPQFY reversed\n                      <jats:italic>Cutibacterium acnes<\/jats:italic>\n                      \u2010induced reductions in keratinocyte viability and proliferation and suppressed the elevation of IL\u20101\u03b2 and TNF\u2010\u03b1. PPQFY also inhibited NET release, as evidenced by decreased levels of MPO\u2010DNA, CitH3, and PADI4. In mice, PPQFY alleviated skin inflammation, reducing keratin thickening, inflammatory infiltration, and Ly6G\n                      <jats:sup>+<\/jats:sup>\n                      CD11b\n                      <jats:sup>+<\/jats:sup>\n                      cell accumulation. These effects were comparable to those of NET inhibitors. PPQFY ameliorates\n                      <jats:italic>Cutibacterium acnes<\/jats:italic>\n                      \u2010induced acne in mice by regulating NETs, providing a novel mechanistic basis for its clinical use and identifying NETs as a potential therapeutic target for acne.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/1348-0421.70071","type":"journal-article","created":{"date-parts":[[2026,6,29]],"date-time":"2026-06-29T11:28:38Z","timestamp":1782732518000},"page":"378-387","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Pipa Qingfei Yin Ameliorates Acne Inflammation in Mice via Suppressing Neutrophil Extracellular Traps"],"prefix":"10.1111","volume":"70","author":[{"ORCID":"https:\/\/orcid.org\/0009-0003-1090-0416","authenticated-orcid":false,"given":"Liangyi","family":"Wang","sequence":"first","affiliation":[{"name":"Department of Traditional Chinese Medicine Central Hospital Affiliated to Shenyang Medical College Shenyang China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaodan","family":"Wu","sequence":"additional","affiliation":[{"name":"Department of Traditional Chinese Medicine Central Hospital Affiliated to Shenyang Medical College Shenyang China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Meihui","family":"Qi","sequence":"additional","affiliation":[{"name":"Department of Traditional Chinese Medicine Central Hospital Affiliated to Shenyang Medical College Shenyang China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shihua","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Traditional Chinese Medicine Central Hospital Affiliated to Shenyang Medical College Shenyang China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,6,29]]},"reference":[{"key":"e_1_2_12_2_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrdp.2015.29"},{"key":"e_1_2_12_3_1","doi-asserted-by":"publisher","DOI":"10.1080\/09546634.2019.1654075"},{"key":"e_1_2_12_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jaad.2016.05.019"},{"key":"e_1_2_12_5_1","doi-asserted-by":"publisher","DOI":"10.12659\/MSM.945336"},{"key":"e_1_2_12_6_1","doi-asserted-by":"publisher","DOI":"10.1189\/jlb.5BT0615-234R"},{"key":"e_1_2_12_7_1","doi-asserted-by":"publisher","DOI":"10.1002\/jlb.59.2.229"},{"key":"e_1_2_12_8_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.1092385"},{"key":"e_1_2_12_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.autrev.2017.09.012"},{"key":"e_1_2_12_10_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41392-024-01933-x"},{"key":"e_1_2_12_11_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-024-51931-w"},{"key":"e_1_2_12_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.hermed.2017.09.001"},{"key":"e_1_2_12_13_1","first-page":"129","article-title":"Network Pharmacology Mechanism of Pipa Qingfei Decoction in the Treatment of Acne","volume":"22","author":"Runan F.","year":"2023","journal-title":"Chinese Journal of Dermatovenereology of Integrated Traditional and Western Medicine"},{"key":"e_1_2_12_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jep.2019.112082"},{"key":"e_1_2_12_15_1","doi-asserted-by":"publisher","DOI":"10.1080\/14786419.2018.1527832"},{"key":"e_1_2_12_16_1","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2025.1643409"},{"key":"e_1_2_12_17_1","first-page":"46","article-title":"Observation on the Therapeutic Effect of Modified Pipa Qingfei Decoction in the Treatment of 48 Acne Cases of Lung Meridian Wind Heat Type (in Chinese)","volume":"3","author":"Hongyan L. G. W. Y. X. M. Z. Q. Z. Q. H. H. W.","year":"2024","journal-title":"Journal of Jiangxi University of Chinese Medicine"},{"key":"e_1_2_12_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jid.2025.02.012"},{"key":"e_1_2_12_19_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms25105302"},{"key":"e_1_2_12_20_1","first-page":"6721","article-title":"Meta\u2010Analysis of Safety and Efficacy of Pipa Qingfei Decoction in Treatment of Acne (in Chinese)","volume":"56","author":"Guang Z. S. Z. W. D. L. Z. W. J.","year":"2025","journal-title":"Chinese Herbal Medicines"},{"key":"e_1_2_12_21_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10753-019-01125-8"},{"key":"e_1_2_12_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.intimp.2024.112708"},{"key":"e_1_2_12_23_1","doi-asserted-by":"publisher","DOI":"10.1038\/nri.2017.105"},{"key":"e_1_2_12_24_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-023-36694-8"}],"container-title":["Microbiology and Immunology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/1348-0421.70071","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1111\/1348-0421.70071","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/1348-0421.70071","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,8,5]],"date-time":"2026-08-05T18:26:24Z","timestamp":1785954384000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/1348-0421.70071"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,6,29]]},"references-count":23,"journal-issue":{"issue":"8","published-print":{"date-parts":[[2026,8]]}},"alternative-id":["10.1111\/1348-0421.70071"],"URL":"https:\/\/doi.org\/10.1111\/1348-0421.70071","archive":["Portico"],"relation":{},"ISSN":["0385-5600","1348-0421"],"issn-type":[{"value":"0385-5600","type":"print"},{"value":"1348-0421","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,6,29]]},"assertion":[{"value":"2026-02-25","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-06-15","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-06-29","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}