{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,8]],"date-time":"2026-09-08T11:12:22Z","timestamp":1788865942220,"version":"build-2803163510"},"reference-count":48,"publisher":"Wiley","issue":"9","license":[{"start":{"date-parts":[[2026,7,6]],"date-time":"2026-07-06T00:00:00Z","timestamp":1783296000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"},{"start":{"date-parts":[[2026,7,6]],"date-time":"2026-07-06T00:00:00Z","timestamp":1783296000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Microbiology and Immunology"],"published-print":{"date-parts":[[2026,9]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    This study aimed to investigate the prevalence and characteristics of\n                    <jats:italic>mcr<\/jats:italic>\n                    \u2010positive Enterobacteriaceae in retail meat in Thailand following the national ban on prophylactic colistin use in food producing animals. A total of 152 meat samples (103 chicken and 49 pork) were collected from supermarkets and open markets between July and September 2023. Samples were screened for\n                    <jats:italic>mcr\u20101<\/jats:italic>\n                    to\n                    <jats:italic>mcr\u20105<\/jats:italic>\n                    using multiplex PCR. None of the samples from supermarkets tested positive, whereas\n                    <jats:italic>mcr<\/jats:italic>\n                    genes were detected in 15.4% (6\/39) and 13.3% (4\/30) of chicken and pork samples, respectively, from open markets, with\n                    <jats:italic>mcr\u20101<\/jats:italic>\n                    and\/or\n                    <jats:italic>mcr\u20103<\/jats:italic>\n                    identified. A total of 21 isolates were recovered from PCR\u2010positive samples (11 from chicken and 10 from pork).\n                    <jats:italic>Escherichia coli<\/jats:italic>\n                    was the predominant species (\n                    <jats:italic>n<\/jats:italic>\n                    \u2009=\u200919), followed by\n                    <jats:italic>Klebsiella pneumoniae<\/jats:italic>\n                    (\n                    <jats:italic>n<\/jats:italic>\n                    \u2009=\u20092). All\n                    <jats:italic>mcr<\/jats:italic>\n                    \u2010positive isolates exhibited multidrug resistance. Whole\u2010genome sequencing was performed for 19 non\u2010clonal isolates. One\n                    <jats:italic>K. pneumoniae<\/jats:italic>\n                    strain from a pork sample co\u2010harbored\n                    <jats:italic>mcr\u20101<\/jats:italic>\n                    and\n                    <jats:italic>mcr\u20108<\/jats:italic>\n                    , representing the first report of this combination in the animal sector in Thailand. In addition, virulence\u2010associated genes, including adhesion factors, toxins, and iron acquisition systems, were identified in selected isolates. Core genome SNP\u2010based phylogenetic analysis revealed substantial genomic diversity among the isolates, suggesting relatedness to strains reported prior to the colistin ban. These findings indicate that retail meat from open markets may serve as an important route for the transmission of\n                    <jats:italic>mcr<\/jats:italic>\n                    \u2010positive bacteria in Thailand and highlight the urgent need to incorporate systematic retail meat surveillance into national antimicrobial resistance monitoring programs.\n                  <\/jats:p>","DOI":"10.1111\/1348-0421.70073","type":"journal-article","created":{"date-parts":[[2026,7,6]],"date-time":"2026-07-06T08:29:29Z","timestamp":1783326569000},"page":"402-415","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Prevalence and Genomic Characterization of\n                    <i>mcr<\/i>\n                    \u2010Positive Enterobacteriaceae in Retail Meat in Thailand Following the Colistin Ban"],"prefix":"10.1111","volume":"70","author":[{"given":"Ahmed","family":"Abououf","sequence":"first","affiliation":[{"name":"Graduate School of Life and Environmental Sciences Osaka Prefecture University Osaka Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sharda Prasad","family":"Awasthi","sequence":"additional","affiliation":[{"name":"Graduate School of Veterinary Science Osaka Metropolitan University Osaka Japan"},{"name":"Osaka International Research Center for Infectious Diseases Osaka Metropolitan University Osaka Japan"},{"name":"Asian Health Science Research Institute Osaka Metropolitan University Osaka Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Asmaa M.","family":"Elbastawesy","sequence":"additional","affiliation":[{"name":"Food Hygiene Department Animal Health Research Institute (AHRI) Kafrelsheikh Egypt"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Noritoshi","family":"Hatanaka","sequence":"additional","affiliation":[{"name":"Graduate School of Life and Environmental Sciences Osaka Prefecture University Osaka Japan"},{"name":"Graduate School of Veterinary Science Osaka Metropolitan University Osaka Japan"},{"name":"Osaka International Research Center for Infectious Diseases Osaka Metropolitan University Osaka Japan"},{"name":"Asian Health Science Research Institute Osaka Metropolitan University Osaka Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Atsushi","family":"Hinenoya","sequence":"additional","affiliation":[{"name":"Graduate School of Life and Environmental Sciences Osaka Prefecture University Osaka Japan"},{"name":"Graduate School of Veterinary Science Osaka Metropolitan University Osaka Japan"},{"name":"Osaka International Research Center for Infectious Diseases Osaka Metropolitan University Osaka Japan"},{"name":"Asian Health Science Research Institute Osaka Metropolitan University Osaka Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Srinuan","family":"Somroop","sequence":"additional","affiliation":[{"name":"Faculty of Allied Health Sciences Thammasat University Bangkok Thailand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yukihiro","family":"Kaneko","sequence":"additional","affiliation":[{"name":"Osaka International Research Center for Infectious Diseases Osaka Metropolitan University Osaka Japan"},{"name":"Graduate School of Medicine Osaka Metropolitan University Osaka Japan"},{"name":"Research Center for Infectious Disease Sciences, Graduate School of Medicine Osaka Metropolitan University Osaka Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shinji","family":"Yamasaki","sequence":"additional","affiliation":[{"name":"Graduate School of Life and Environmental Sciences Osaka Prefecture University Osaka Japan"},{"name":"Graduate School of Veterinary Science Osaka Metropolitan University Osaka Japan"},{"name":"Osaka International Research Center for Infectious Diseases Osaka Metropolitan University Osaka Japan"},{"name":"Asian Health Science Research Institute Osaka Metropolitan University Osaka Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,7,6]]},"reference":[{"key":"e_1_2_11_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(21)027"},{"key":"e_1_2_11_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(24)02081-6"},{"key":"e_1_2_11_4_1","doi-asserted-by":"publisher","DOI":"10.1517\/13543784.17.7.973"},{"key":"e_1_2_11_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1473-3099(15)00424-7"},{"key":"e_1_2_11_6_1","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/dkaa205"},{"key":"e_1_2_11_7_1","doi-asserted-by":"publisher","DOI":"10.1080\/22221751.2020.1732231"},{"key":"e_1_2_11_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijfoodmicro.2021.109164"},{"key":"e_1_2_11_9_1","doi-asserted-by":"publisher","DOI":"10.1128\/Spectrum.00025-21"},{"key":"e_1_2_11_10_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41426-018-0033-1"},{"key":"e_1_2_11_11_1","unstructured":"Ipsos Business Consulting Thailand's Poultry Industry Ipsos \u201d2013 https:\/\/www.ipsos.com\/sites\/default\/files\/ct\/publication\/documents\/2018-09\/thailands_poultry_industry-sep2013.pdf."},{"key":"e_1_2_11_12_1","unstructured":"USDA; United States Department of Agriculture Foreign Agricultural Service.2025. Livestock and poultry: World Markets and Trade https:\/\/apps.fas.usda.gov\/psdonline\/circulars\/livestock_poultry.pdf."},{"key":"e_1_2_11_13_1","doi-asserted-by":"publisher","DOI":"10.1089\/mdr.2020.0242"},{"key":"e_1_2_11_14_1","doi-asserted-by":"publisher","DOI":"10.3389\/fvets.2020.582899"},{"key":"e_1_2_11_15_1","doi-asserted-by":"publisher","DOI":"10.1099\/mgen.0.000951"},{"key":"e_1_2_11_16_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0238939"},{"key":"e_1_2_11_17_1","unstructured":"Thailand One Health Report. (2017). Antimicrobial Consumption and Antimicrobial Resistance https:\/\/rr-asia.woah.org\/app\/uploads\/2020\/10\/thailand_s-one-health-report-in-2017.pdf."},{"key":"e_1_2_11_18_1","doi-asserted-by":"publisher","DOI":"10.3389\/fcimb.2022.941358"},{"key":"e_1_2_11_19_1","doi-asserted-by":"publisher","DOI":"10.2807\/1560-7917.ES.2018.23.6.17-00672"},{"key":"e_1_2_11_20_1","unstructured":"A.Nahar Prevalence and Characterization of Extended\u2010Spectrum Beta\u2010Lactamase\u2010ProducingEscherichia coliand mcr\u20101 Gene\u2010Positive E. coli in Domestic and Imported Chickens in Japan (PhD Dissertation) Osaka Prefecture University Japan \u201d2018."},{"key":"e_1_2_11_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijantimicag.2017.11.010"},{"key":"e_1_2_11_22_1","doi-asserted-by":"publisher","DOI":"10.1093\/jambio\/lxad123"},{"key":"e_1_2_11_23_1","volume-title":"Edwards and Ewing's Identification of Enterobacteriaceae","author":"Ewing W. H.","year":"1986"},{"key":"e_1_2_11_24_1","unstructured":"CLSI Performance Standards for Antimicrobial Susceptibility Testing. Twenty\u2010Eighth Informational Supplement CLSI Document M100\u2010S28. Wayne PA: CLSI \u201d2018 https:\/\/clsi.org."},{"key":"e_1_2_11_25_1","doi-asserted-by":"publisher","DOI":"10.1089\/fpd.2015.1954"},{"key":"e_1_2_11_26_1","doi-asserted-by":"publisher","DOI":"10.1292\/jvms.16\u20100639"},{"key":"e_1_2_11_27_1","doi-asserted-by":"publisher","DOI":"10.1292\/jvms.17-0708"},{"key":"e_1_2_11_28_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijfoodmicro.2008.06.017"},{"key":"e_1_2_11_29_1","unstructured":"T.Seemann(2015). Snippy: Fast Bacterial Variant Calling From NGS Reads Available athttps:\/\/github.com\/tseemann\/snippy."},{"key":"e_1_2_11_30_1","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2020.586368"},{"key":"e_1_2_11_31_1","doi-asserted-by":"publisher","DOI":"10.3389\/fcimb.2024.1289134"},{"key":"e_1_2_11_32_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gku1196"},{"key":"e_1_2_11_33_1","doi-asserted-by":"publisher","DOI":"10.1093\/molbev\/msu300"},{"key":"e_1_2_11_34_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkae268"},{"key":"e_1_2_11_35_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrmicro3093"},{"key":"e_1_2_11_36_1","doi-asserted-by":"publisher","DOI":"10.3136\/fstr.18.705"},{"key":"e_1_2_11_37_1","doi-asserted-by":"publisher","DOI":"10.3389\/fvets.2024.1375127"},{"key":"e_1_2_11_38_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.envint.2018.11.021"},{"key":"e_1_2_11_39_1","doi-asserted-by":"publisher","DOI":"10.14202\/vetworld.2023.1527-1533"},{"key":"e_1_2_11_40_1","doi-asserted-by":"publisher","DOI":"10.4142\/jvs.2021.22.e68"},{"key":"e_1_2_11_41_1","doi-asserted-by":"publisher","DOI":"10.3390\/antibiotics14060596"},{"key":"e_1_2_11_42_1","unstructured":"Thailand One Health Report. (2021). Antimicrobial Consumption and Antimicrobial Resistance https:\/\/www.fda.moph.go.th\/sites\/antimicrobial\/SitePages\/publication.aspx."},{"key":"e_1_2_11_43_1","doi-asserted-by":"publisher","DOI":"10.3389\/fcimb.2023.1178547"},{"key":"e_1_2_11_44_1","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/dkad097"},{"key":"e_1_2_11_45_1","doi-asserted-by":"publisher","DOI":"10.2147\/IDR.S209473"},{"key":"e_1_2_11_46_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jiph.2024.102624"},{"key":"e_1_2_11_47_1","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2020.00080"},{"key":"e_1_2_11_48_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijantimicag.2021.106303"},{"key":"e_1_2_11_49_1","doi-asserted-by":"publisher","DOI":"10.3201\/eid0502.990220"}],"container-title":["Microbiology and Immunology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/1348-0421.70073","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1111\/1348-0421.70073","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/1348-0421.70073","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,9,8]],"date-time":"2026-09-08T10:56:31Z","timestamp":1788864991000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/1348-0421.70073"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,7,6]]},"references-count":48,"journal-issue":{"issue":"9","published-print":{"date-parts":[[2026,9]]}},"alternative-id":["10.1111\/1348-0421.70073"],"URL":"https:\/\/doi.org\/10.1111\/1348-0421.70073","archive":["Portico"],"relation":{},"ISSN":["0385-5600","1348-0421"],"issn-type":[{"value":"0385-5600","type":"print"},{"value":"1348-0421","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,7,6]]},"assertion":[{"value":"2026-05-14","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-06-16","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-07-06","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}