{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,8]],"date-time":"2026-09-08T11:12:35Z","timestamp":1788865955224,"version":"build-2803163510"},"reference-count":59,"publisher":"Wiley","issue":"9","license":[{"start":{"date-parts":[[2026,7,17]],"date-time":"2026-07-17T00:00:00Z","timestamp":1784246400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,7,17]],"date-time":"2026-07-17T00:00:00Z","timestamp":1784246400000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Microbiology and Immunology"],"published-print":{"date-parts":[[2026,9]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Human herpesvirus 6B (HHV\u20106B) entry is mediated by the interaction between gH\/gL\/gQ1\/gQ2 complex (tetramer) and CD134 expressed on activated T cells. In this study, we identified and characterized a panel of monoclonal antibodies targeting the HHV\u20106B tetramer, with particular focus on three antibodies; gHgL J1, gHgL O2, and gHgL F5; that represent anti\u2010gH neutralizing monoclonal antibodies with binding characteristics distinct from the previously described antibody OHV\u20103. Immunofluorescence and Western blot analyses confirmed recognition of the HHV\u20106B tetramer in infected and transfected cells. Biolayer interferometry (BLI) demonstrated that these antibodies exhibited dissociation constants (\n                    <jats:italic>K<\/jats:italic>\n                    <jats:sub>\n                      <jats:italic>D<\/jats:italic>\n                    <\/jats:sub>\n                    ) in the range of 62.52\u201376.99\u2009nM. We next assessed the effect of antibodies on the interaction between the HHV\u20106B tetramer and CD134. None of the gH\u2010targeting antibodies inhibited the CD134 binding. Competition analysis using BLI\u2010based antibody\u2010Fab competition assays indicated that gHgL J1, gHgL O2, and gHgL F5 form a competition\u2010defined antigenic cluster distinct from that recognized by OHV\u20103. Collectively, these findings identify a group of anti\u2010gH antibodies which neutralizes HHV\u20106B with CD134\u2010binding\u2010independent profiles and suggest the presence of at least two distinguishable antibody\u2010recognition regions within HHV\u20106B gH. These results expand our understanding of the antigenic organization of the HHV\u20106B entry complex.\n                  <\/jats:p>","DOI":"10.1111\/1348-0421.70076","type":"journal-article","created":{"date-parts":[[2026,7,17]],"date-time":"2026-07-17T11:41:36Z","timestamp":1784288496000},"page":"448-461","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Identification of Two Competition\u2010Defined Neutralizing Antibody Groups Targeting the HHV\u20106B gH\/gL\/gQ1\/gQ2 Complex Independent of CD134 Binding"],"prefix":"10.1111","volume":"70","author":[{"given":"Achmad Januar Er","family":"Putra","sequence":"first","affiliation":[{"name":"Division of Clinical Virology, Center for Infectious Diseases Kobe University Graduate School of Medicine Kobe Hyogo Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ida Ayu Gde Wahyudevi","family":"Dharmika","sequence":"additional","affiliation":[{"name":"Division of Clinical Virology, Center for Infectious Diseases Kobe University Graduate School of Medicine Kobe Hyogo Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Natsumi","family":"Hasegawa","sequence":"additional","affiliation":[{"name":"Division of Clinical Virology, Center for Infectious Diseases Kobe University Graduate School of Medicine Kobe Hyogo Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Regina Rachel","family":"Gunawan","sequence":"additional","affiliation":[{"name":"Division of Clinical Virology, Center for Infectious Diseases Kobe University Graduate School of Medicine Kobe Hyogo Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rei","family":"Takamiya","sequence":"additional","affiliation":[{"name":"Division of Clinical Virology, Center for Infectious Diseases Kobe University Graduate School of Medicine Kobe Hyogo 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