{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,8]],"date-time":"2026-09-08T11:12:26Z","timestamp":1788865946187,"version":"build-2803163510"},"reference-count":34,"publisher":"Wiley","issue":"9","license":[{"start":{"date-parts":[[2026,7,22]],"date-time":"2026-07-22T00:00:00Z","timestamp":1784678400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,7,22]],"date-time":"2026-07-22T00:00:00Z","timestamp":1784678400000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Microbiology and Immunology"],"published-print":{"date-parts":[[2026,9]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    A C\u2010type lectin with specificity for N\u2010acetyl\u2010\n                    <jats:sc>d<\/jats:sc>\n                    \u2010glucosamine was purified from the cutaneous mucus of chub mackerel (\n                    <jats:italic>Scomber japonicus<\/jats:italic>\n                    ) by affinity chromatography. The lectin comprises 159 amino acid residues and contains an unusual substitution within the conserved Ca\n                    <jats:sup>2+<\/jats:sup>\n                    \u2010binding motif, in which Trp\u2013Asn\u2013Asp is replaced by Trp\u2013Ser\u2013Asp. Reverse transcription\u2013PCR analysis demonstrated that the lectin gene is specifically expressed in the skin, and immunohistochemistry localized the protein to the outer epidermal layer, predominantly in squamous cells. Agglutinating activity was observed against\n                    <jats:italic>L. garvieae<\/jats:italic>\n                    , but not against any of the other bacterial species tested. An approximately 25\u2010kDa bacterial protein was identified as a potential ligand, although its identity remains unresolved. Collectively, these findings indicate that this lectin (SjCL) functions as a mucosal immune molecule and may contribute to the first line of defense against bacterial pathogens in teleost fish.\n                  <\/jats:p>","DOI":"10.1111\/1348-0421.70078","type":"journal-article","created":{"date-parts":[[2026,7,22]],"date-time":"2026-07-22T11:59:28Z","timestamp":1784721568000},"page":"436-447","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["A\n                    <i>N<\/i>\n                    \u2010Acetyl\u2010D\u2010Glucosamine\u2010Binding C\u2010Type Lectin in the Cutaneous Mucus of Chub Mackerel,\n                    <i>Scomber japonicus<\/i>\n                    , Agglutinates Pathogenic Bacteria"],"prefix":"10.1111","volume":"70","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-2875-0543","authenticated-orcid":false,"given":"Shigeyuki","family":"Tsutsui","sequence":"first","affiliation":[{"name":"Laboratory of Fish Pathology, School of Marine Biosciences Kitasato University Sagamihara Kanagawa Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Takuya","family":"Kunisaki","sequence":"additional","affiliation":[{"name":"Laboratory of Fish Pathology, School of Marine Biosciences Kitasato University Sagamihara Kanagawa Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tatsuto","family":"Izawa","sequence":"additional","affiliation":[{"name":"Laboratory of Fish Pathology, School of Marine Biosciences Kitasato University Sagamihara Kanagawa Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Osamu","family":"Nakamura","sequence":"additional","affiliation":[{"name":"Laboratory of Fish Pathology, School of Marine Biosciences Kitasato University Sagamihara Kanagawa Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,7,22]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1096-4959(03)00178-7"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M202648200"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.molimm.2006.04.023"},{"key":"e_1_2_10_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.dci.2023.105113"},{"key":"e_1_2_10_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/0304-4165(92)90109-8"},{"key":"e_1_2_10_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0305-0491(99)00037-1"},{"key":"e_1_2_10_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.dci.2003.08.006"},{"key":"e_1_2_10_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.fsi.2011.05.006"},{"key":"e_1_2_10_10_1","doi-asserted-by":"publisher","DOI":"10.3389\/fcimb.2025.1572734"},{"key":"e_1_2_10_11_1","doi-asserted-by":"publisher","DOI":"10.1002\/pmic.201500120"},{"key":"e_1_2_10_12_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M301038200"},{"key":"e_1_2_10_13_1","doi-asserted-by":"publisher","DOI":"10.1093\/jb\/mvr085"},{"key":"e_1_2_10_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbrc.2005.05.118"},{"key":"e_1_2_10_15_1","doi-asserted-by":"publisher","DOI":"10.2307\/1443164"},{"key":"e_1_2_10_16_1","doi-asserted-by":"publisher","DOI":"10.3354\/meps08832"},{"key":"e_1_2_10_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.aaf.2022.03.003"},{"key":"e_1_2_10_18_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12562-011-0332-0"},{"key":"e_1_2_10_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.dci.2020.103748"},{"key":"e_1_2_10_20_1","doi-asserted-by":"publisher","DOI":"10.3314\/jjmm.39.147"},{"key":"e_1_2_10_21_1","doi-asserted-by":"publisher","DOI":"10.1038\/360183a0"},{"key":"e_1_2_10_22_1","doi-asserted-by":"publisher","DOI":"10.1038\/360127a0"},{"key":"e_1_2_10_23_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1742-4658.2005.05031.x"},{"key":"e_1_2_10_24_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.fsi.2012.09.021"},{"key":"e_1_2_10_25_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.dci.2011.12.010"},{"key":"e_1_2_10_26_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.dci.2025.105416"},{"key":"e_1_2_10_27_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.fsi.2014.12.035"},{"key":"e_1_2_10_28_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.fsi.2024.109833"},{"key":"e_1_2_10_29_1","doi-asserted-by":"publisher","DOI":"10.1042\/bj3600107"},{"key":"e_1_2_10_30_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1574-6976.2007.00094.x"},{"key":"e_1_2_10_31_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev-micro-092412-155620"},{"key":"e_1_2_10_32_1","doi-asserted-by":"publisher","DOI":"10.1101\/cshperspect.a000414"},{"key":"e_1_2_10_33_1","volume-title":"Essentials of Glycobiology","author":"Varki A.","year":"2017"},{"key":"e_1_2_10_34_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cimid.2006.06.003"},{"key":"e_1_2_10_35_1","doi-asserted-by":"publisher","DOI":"10.3354\/dao03083"}],"container-title":["Microbiology and Immunology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/1348-0421.70078","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1111\/1348-0421.70078","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/1348-0421.70078","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,9,8]],"date-time":"2026-09-08T10:56:45Z","timestamp":1788865005000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/1348-0421.70078"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,7,22]]},"references-count":34,"journal-issue":{"issue":"9","published-print":{"date-parts":[[2026,9]]}},"alternative-id":["10.1111\/1348-0421.70078"],"URL":"https:\/\/doi.org\/10.1111\/1348-0421.70078","archive":["Portico"],"relation":{},"ISSN":["0385-5600","1348-0421"],"issn-type":[{"value":"0385-5600","type":"print"},{"value":"1348-0421","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,7,22]]},"assertion":[{"value":"2026-05-08","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-07-07","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-07-22","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}