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We recently demonstrated that CCHC\u2010type zinc\u2010finger\u2010containing protein 3 (ZCCHC3) suppresses human immunodeficiency virus type 1 (HIV\u20101) replication through multiple mechanisms. We also revealed that single\u2010nucleotide polymorphisms in human ZCCHC3 affect its antiviral function; however, whether similar genetic and functional diversity is present in other species remains unknown. In this study, we investigated the genetic and functional diversity of ZCCHC3 in cynomolgus macaques, a critical animal model for HIV\u20101\u2010related research. Sequencing analysis of eight independent ZCCHC3 clones per animal revealed substantial amino acid diversity among cynomolgus macaques. We selected 12 representative variants and examined their antiviral activity against several retroviral vectors derived from HIV\u20101, simian immunodeficiency virus, feline immunodeficiency virus, and murine leukemia virus. 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