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The T3SS gatekeeper protein BopN controls BteA translocation, yet the BteA region mediating this regulation remains unknown. Here, we demonstrate that BteA interacts with BopN through its C\u2010terminal region, and this interaction is required for efficient translocation into host cells. Using a CyaA\u2010based translocation assay, we showed that BteA comprising the N\u2010terminal 448 amino acids (BteA\u2010N448) and BteA with a deletion of amino acids 249\u2013448 (BteA\u2010\u2206249\u2013448) were translocated in a BopN\u2010dependent manner, whereas BteA comprising the N\u2010terminal 248 amino acids (BteA\u2010N248) was translocated independently of BopN. Pull\u2010down assays confirmed that BteA\u2010N448 and BteA\u2010\u0394249\u2013448 physically interact with BopN, whereas BteA\u2010N248 does not. ColabFold\u2010based structure prediction identified candidate interface residues, and subsequent mutagenesis revealed that simultaneous alanine substitution of aspartate 263 (D263) and phenylalanine 340 (F340) abolished BopN\u2010dependent translocation. Our results demonstrated that the amino acid residues D263 and F340 in BteA play important roles in BopN\u2010mediated translocation control. Furthermore, the C\u2010terminal 14\u2010amino\u2010acid region of BteA was strongly suggested to be involved in this regulation. The present study provides new insights into the effector\u2010gatekeeper network that controls T3SS\u2010mediated pathogenicity in\n                    <jats:italic>Bordetella<\/jats:italic>\n                    .\n                  <\/jats:p>","DOI":"10.1111\/1348-0421.70093","type":"journal-article","created":{"date-parts":[[2026,9,29]],"date-time":"2026-09-29T07:30:55Z","timestamp":1790667055000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["The\n                    <i>Bordetella<\/i>\n                    Effector BteA Interacts With BopN Through Its C\u2010Terminal Region, Thereby Controlling Its Translocation Into Host Cells"],"prefix":"10.1111","author":[{"ORCID":"https:\/\/orcid.org\/0009-0007-3516-467X","authenticated-orcid":false,"given":"Toshinobu","family":"Ogawa","sequence":"first","affiliation":[{"name":"Laboratory of Bacterial Infection, Graduate School of Infection Control Sciences Kitasato 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