{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,9,19]],"date-time":"2025-09-19T09:30:32Z","timestamp":1758274232649,"version":"3.37.3"},"reference-count":34,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2014,10,30]],"date-time":"2014-10-30T00:00:00Z","timestamp":1414627200000},"content-version":"vor","delay-in-days":29,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100004097","name":"Fondation ARC pour la recherche sur le cancer","doi-asserted-by":"crossref","id":[{"id":"10.13039\/501100004097","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100004099","name":"Ligue contre le cancer","doi-asserted-by":"crossref","id":[{"id":"10.13039\/501100004099","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Clin Exp Pharma Physio"],"published-print":{"date-parts":[[2014,10]]},"abstract":"<jats:title>Summary<\/jats:title><jats:p>Nucleotide excision repair (<jats:styled-content style=\"fixed-case\">NER<\/jats:styled-content>) is involved in the repair of <jats:styled-content style=\"fixed-case\">DNA<\/jats:styled-content> damage caused by platinum derivatives and has been shown to decrease the cytotoxic activity of these drugs. Because protein\u2013protein interactions are essential for <jats:styled-content style=\"fixed-case\">NER<\/jats:styled-content> activity, we transfected human cancer cell lines (A549 and <jats:styled-content style=\"fixed-case\">HCT<\/jats:styled-content>116) with plasmids coding the amino acid sequences corresponding to the interacting domains between excision repair cross\u2010complementation group 1 (<jats:styled-content style=\"fixed-case\">ERCC<\/jats:styled-content>1) and xeroderma pigmentosum, complementation group A (<jats:styled-content style=\"fixed-case\">XPA<\/jats:styled-content>), as well as <jats:styled-content style=\"fixed-case\">ERCC<\/jats:styled-content>1 and xeroderma pigmentosum, complementation group F (<jats:styled-content style=\"fixed-case\">XPF<\/jats:styled-content>), all <jats:styled-content style=\"fixed-case\">NER<\/jats:styled-content> proteins. Using the 3\u2010(4,5\u2010dimethyl\u20102 thiazoyl)\u20102,5\u2010diphenyl\u20102H\u2010tetrazolium bromide (<jats:styled-content style=\"fixed-case\">MTT<\/jats:styled-content>) assay and annexin V staining, we showed that transfected A549 cells were sensitized 1.2\u20132.2\u2010fold to carboplatin and that transfected <jats:styled-content style=\"fixed-case\">HCT<\/jats:styled-content>116 cells were sensitized 1.4\u20135.4\u2010fold to oxaliplatin <jats:italic>in vitro<\/jats:italic>. In addition, transfected cells exhibited modified <jats:italic>in vivo<\/jats:italic> sensitivity to the same drugs. Finally, in particular cell models of the interaction between <jats:styled-content style=\"fixed-case\">ERCC<\/jats:styled-content>1 and <jats:styled-content style=\"fixed-case\">XPF<\/jats:styled-content>,<jats:styled-content style=\"fixed-case\"> DNA<\/jats:styled-content> repair was decreased, as evidenced by increased phosphorylation of the histone 2<jats:styled-content style=\"fixed-case\">AX<\/jats:styled-content> after exposure to mitomycin C, and genomic instability was increased, as determined by comparative genomic hybridization studies. The results indicate that the interacting peptides act as dominant negatives and decrease <jats:styled-content style=\"fixed-case\">NER<\/jats:styled-content> activity through inhibition of protein\u2013protein interactions.<\/jats:p>","DOI":"10.1111\/1440-1681.12282","type":"journal-article","created":{"date-parts":[[2014,8,12]],"date-time":"2014-08-12T12:37:40Z","timestamp":1407847060000},"page":"817-824","source":"Crossref","is-referenced-by-count":7,"title":["Expression of domains for protein\u2013protein interaction of nucleotide excision repair proteins modifies cancer cell sensitivity to platinum derivatives and genomic stability"],"prefix":"10.1111","volume":"41","author":[{"given":"Lars Petter","family":"Jordheim","sequence":"first","affiliation":[{"name":"Universit\u00e9 de Lyon Lyon France"},{"name":"Universit\u00e9 de Lyon 1 Lyon France"},{"name":"Cancer Research Center in Lyon INSERM U1052 Lyon France"},{"name":"Cancer Research Center in Lyon CNRS UMR 5286 Lyon France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Emeline","family":"Cros\u2010Perrial","sequence":"additional","affiliation":[{"name":"Universit\u00e9 de Lyon Lyon France"},{"name":"Universit\u00e9 de Lyon 1 Lyon France"},{"name":"Cancer Research Center in Lyon INSERM U1052 Lyon France"},{"name":"Cancer Research Center in Lyon CNRS UMR 5286 Lyon France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Eva\u2010Laure","family":"Matera","sequence":"additional","affiliation":[{"name":"Universit\u00e9 de Lyon Lyon France"},{"name":"Universit\u00e9 de Lyon 1 Lyon France"},{"name":"Cancer Research Center in Lyon INSERM U1052 Lyon France"},{"name":"Cancer Research Center in Lyon CNRS UMR 5286 Lyon France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Karima","family":"Bouledrak","sequence":"additional","affiliation":[{"name":"Universit\u00e9 de Lyon Lyon France"},{"name":"Universit\u00e9 de Lyon 1 Lyon France"},{"name":"Cancer Research Center in Lyon INSERM U1052 Lyon France"},{"name":"Cancer Research Center in Lyon CNRS UMR 5286 Lyon France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Charles","family":"Dumontet","sequence":"additional","affiliation":[{"name":"Universit\u00e9 de Lyon Lyon France"},{"name":"Universit\u00e9 de Lyon 1 Lyon France"},{"name":"Cancer Research Center in Lyon INSERM U1052 Lyon France"},{"name":"Cancer Research Center in Lyon CNRS UMR 5286 Lyon France"},{"name":"Hematology Department Hospices Civils de Lyon Pierre B\u00e9nite France"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2014,10,30]]},"reference":[{"doi-asserted-by":"publisher","key":"e_1_2_7_2_1","DOI":"10.1016\/j.ctrv.2007.07.001"},{"doi-asserted-by":"publisher","key":"e_1_2_7_3_1","DOI":"10.1038\/onc.2011.384"},{"doi-asserted-by":"publisher","key":"e_1_2_7_4_1","DOI":"10.1038\/cr.2008.2"},{"doi-asserted-by":"publisher","key":"e_1_2_7_5_1","DOI":"10.1073\/pnas.91.11.5012"},{"doi-asserted-by":"publisher","key":"e_1_2_7_6_1","DOI":"10.1093\/nar\/26.18.4146"},{"doi-asserted-by":"publisher","key":"e_1_2_7_7_1","DOI":"10.1021\/bi990591"},{"doi-asserted-by":"publisher","key":"e_1_2_7_8_1","DOI":"10.1038\/sj.emboj.7601894"},{"doi-asserted-by":"publisher","key":"e_1_2_7_9_1","DOI":"10.1016\/j.str.2005.08.014"},{"doi-asserted-by":"publisher","key":"e_1_2_7_10_1","DOI":"10.1002\/prot.21635"},{"doi-asserted-by":"publisher","key":"e_1_2_7_11_1","DOI":"10.1016\/j.bpj.2013.04.023"},{"doi-asserted-by":"publisher","key":"e_1_2_7_12_1","DOI":"10.1074\/jbc.M111.337899"},{"doi-asserted-by":"publisher","key":"e_1_2_7_13_1","DOI":"10.1016\/j.dnarep.2008.03.015"},{"doi-asserted-by":"publisher","key":"e_1_2_7_14_1","DOI":"10.1016\/j.dnarep.2009.10.005"},{"key":"e_1_2_7_15_1","first-page":"1311","article-title":"Enhanced cisplatin cytotoxicity by disturbing the nucleotide excision repair pathway in ovarian cancer cell lines","volume":"63","author":"Selvakumaran M","year":"2003","journal-title":"Cancer Res."},{"doi-asserted-by":"publisher","key":"e_1_2_7_16_1","DOI":"10.1016\/j.dnarep.2010.03.010"},{"doi-asserted-by":"publisher","key":"e_1_2_7_17_1","DOI":"10.1016\/j.bcp.2006.04.025"},{"doi-asserted-by":"publisher","key":"e_1_2_7_18_1","DOI":"10.1016\/j.bbrc.2004.12.008"},{"doi-asserted-by":"publisher","key":"e_1_2_7_19_1","DOI":"10.1186\/1476-4598-9-248"},{"doi-asserted-by":"publisher","key":"e_1_2_7_20_1","DOI":"10.1158\/1078-0432.CCR-10-0593"},{"doi-asserted-by":"publisher","key":"e_1_2_7_21_1","DOI":"10.1158\/0008-5472.CAN-04-0348"},{"doi-asserted-by":"publisher","key":"e_1_2_7_22_1","DOI":"10.1056\/NEJMoa060570"},{"key":"e_1_2_7_23_1","first-page":"2286","article-title":"Low ERCC1 expression correlates with prolonged survival after cisplatin plus gemcitabine chemotherapy in non\u2010small cell lung cancer","volume":"8","author":"Lord RV","year":"2002","journal-title":"Clin. 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