{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,21]],"date-time":"2026-01-21T07:54:09Z","timestamp":1768982049943,"version":"3.49.0"},"reference-count":22,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2014,10,30]],"date-time":"2014-10-30T00:00:00Z","timestamp":1414627200000},"content-version":"vor","delay-in-days":29,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"Basel Hetzel Institute Program Grant (Adelaide, Australia)","award":["200904"],"award-info":[{"award-number":["200904"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Clin Exp Pharma Physio"],"published-print":{"date-parts":[[2014,10]]},"abstract":"<jats:title>Summary<\/jats:title><jats:p>Many acute cardiovascular disease states are associated with neutrophil infiltration of myocardium and subsequent release of superoxide (O<jats:sub>2<\/jats:sub><jats:sup>\u2212<\/jats:sup>) and myeloperoxidase (MPO), which contribute to inflammatory reactions. B\u2010Type natriuretic peptide (BNP) is known to exert anti\u2010inflammatory and antifibrotic effects, but it is not known whether these may include interactions with neutrophils. In neutrophils isolated from 20 healthy subjects, we assessed the effect of BNP on the \u2018neutrophil burst\u2019 (O<jats:sub>2<\/jats:sub><jats:sup>\u2212<\/jats:sup> production and MPO release) stimulated by phorbol myristate acetate (PMA) and <jats:italic>N<\/jats:italic>\u2010formyl\u2010methionyl\u2010leucyl\u2010phenylalanine (<jats:styled-content style=\"fixed-case\">fMLP<\/jats:styled-content>), respectively. Effects of BNP on <jats:styled-content style=\"fixed-case\">cGMP<\/jats:styled-content> accumulation, and the effects of the cell\u2010permeable <jats:styled-content style=\"fixed-case\">cGMP<\/jats:styled-content> analogue 8\u2010(4\u2010chlorophenylthio) guanosine\u2010<jats:styled-content style=\"fixed-case\">cGMP<\/jats:styled-content> (8\u2010p\u2010CPT\u2010<jats:styled-content style=\"fixed-case\">cGMP<\/jats:styled-content>) and protein kinase G (PKG) inhibition with KT5823 on the neutrophil\u2013BNP interaction were also evaluated. B\u2010Type natriuretic peptide suppressed O<jats:sub>2<\/jats:sub><jats:sup>\u2212<\/jats:sup> release from neutrophils by 23\u00a0\u00b1\u00a06% (<jats:italic>P\u00a0<\/jats:italic>&lt;\u00a00.001) and 24\u00a0\u00b1\u00a08% (<jats:italic>P\u00a0<\/jats:italic>&lt;\u00a00.05) following PMA and <jats:styled-content style=\"fixed-case\">fMLP<\/jats:styled-content> stimulation, respectively. Although BNP did not significantly increase <jats:styled-content style=\"fixed-case\">cGMP<\/jats:styled-content> formation, 8\u2010p\u2010CPT\u2010<jats:styled-content style=\"fixed-case\">cGMP<\/jats:styled-content> suppressed both PMA\u2010 and <jats:styled-content style=\"fixed-case\">fMLP<\/jats:styled-content>\u2010induced neutrophil O<jats:sub>2<\/jats:sub><jats:sup>\u2212<\/jats:sup> release by 16% and 28%, respectively (<jats:italic>P\u00a0<\/jats:italic>&lt;\u00a00.05). The PKG inhibitor KT5823 attenuated the effects of BNP on both <jats:styled-content style=\"fixed-case\">fMLP<\/jats:styled-content>\u2010 and PMA\u2010associated O<jats:sub>2<\/jats:sub><jats:sup>\u2212<\/jats:sup> production. Neither BNP nor 8\u2010p\u2010CPT\u2010<jats:styled-content style=\"fixed-case\">cGMP<\/jats:styled-content> significantly affected MPO release from neutrophils. Suppression of O<jats:sub>2<\/jats:sub><jats:sup>\u2212<\/jats:sup> release from neutrophils by BNP may contribute to its anti\u2010inflammatory and antifibrotic actions.<\/jats:p>","DOI":"10.1111\/1440-1681.12291","type":"journal-article","created":{"date-parts":[[2014,8,12]],"date-time":"2014-08-12T12:38:18Z","timestamp":1407847098000},"page":"739-743","source":"Crossref","is-referenced-by-count":8,"title":["B\u2010Type natriuretic peptide suppression of neutrophil superoxide generation: mechanistic studies in normal subjects"],"prefix":"10.1111","volume":"41","author":[{"given":"Saifei","family":"Liu","sequence":"first","affiliation":[{"name":"Cardiology Unit Basil Hetzel Institute The Queen Elizabeth Hospital University of Adelaide Adelaide SA Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Doan TM","family":"Ngo","sequence":"additional","affiliation":[{"name":"Cardiology Unit Basil Hetzel Institute The Queen Elizabeth Hospital University of Adelaide Adelaide SA Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Simon","family":"Stewart","sequence":"additional","affiliation":[{"name":"Baker IDI Heart and Diabetes Institute Melbourne Vic. Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"John D","family":"Horowitz","sequence":"additional","affiliation":[{"name":"Cardiology Unit Basil Hetzel Institute The Queen Elizabeth Hospital University of Adelaide Adelaide SA Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yuliy Y","family":"Chirkov","sequence":"additional","affiliation":[{"name":"Cardiology Unit Basil Hetzel Institute The Queen Elizabeth Hospital University of Adelaide Adelaide SA Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2014,10,30]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.freeradbiomed.2009.04.004"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0089307"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJM198902093200606"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1172\/JCI115929"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1476-5381.1993.tb13653.x"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1053\/pcad.2002.24599"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1155\/2012\/962347"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1136\/hrt.2005.069286"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpcell.1993.265.1.C201"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.175.3.1919"},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF00179041"},{"key":"e_1_2_7_13_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF00172578"},{"key":"e_1_2_7_14_1","doi-asserted-by":"publisher","DOI":"10.1172\/JCI118660"},{"key":"e_1_2_7_15_1","doi-asserted-by":"publisher","DOI":"10.1172\/JCI115752"},{"key":"e_1_2_7_16_1","doi-asserted-by":"publisher","DOI":"10.1177\/153537020523000403"},{"key":"e_1_2_7_17_1","doi-asserted-by":"publisher","DOI":"10.1085\/jgp.200509403"},{"key":"e_1_2_7_18_1","doi-asserted-by":"publisher","DOI":"10.1161\/01.CIR.0000019121.91548.C2"},{"key":"e_1_2_7_19_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0034892"},{"key":"e_1_2_7_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cardiores.2006.07.006"},{"key":"e_1_2_7_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ejphar.2012.05.006"},{"key":"e_1_2_7_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0003-9861(02)00064-4"},{"key":"e_1_2_7_23_1","doi-asserted-by":"publisher","DOI":"10.1016\/0003-9861(82)90097-2"}],"container-title":["Clinical and Experimental Pharmacology and Physiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2F1440-1681.12291","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/1440-1681.12291","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,3]],"date-time":"2023-10-03T00:33:46Z","timestamp":1696293226000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/1440-1681.12291"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2014,10]]},"references-count":22,"journal-issue":{"issue":"10","published-print":{"date-parts":[[2014,10]]}},"alternative-id":["10.1111\/1440-1681.12291"],"URL":"https:\/\/doi.org\/10.1111\/1440-1681.12291","archive":["Portico"],"relation":{},"ISSN":["0305-1870","1440-1681"],"issn-type":[{"value":"0305-1870","type":"print"},{"value":"1440-1681","type":"electronic"}],"subject":[],"published":{"date-parts":[[2014,10]]}}}