{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,23]],"date-time":"2026-08-23T18:14:38Z","timestamp":1787508878501,"version":"build-2736575974"},"reference-count":65,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2015,2,11]],"date-time":"2015-02-11T00:00:00Z","timestamp":1423612800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Clin Exp Pharma Physio"],"published-print":{"date-parts":[[2015,3]]},"abstract":"<jats:title>Summary<\/jats:title>\n                  <jats:p>\n                    Dipyrone is a non\u2010steroidal anti\u2010inflammatory drug used primarily as an analgesic and antipyretic. Some hypothesize that dipyrone activity can modulate other pathways, including endocannabinoid signalling. Thus, the aim of the present study was to evaluate the possible role of endocannabinoids in mediating dipyrone activity. This study is based on the tetrad effects of cannabinoids, namely an antinociceptive and cataleptic state, hypolocomotion and hypothermia. Dipyrone (500\u00a0mg\/kg, i.p.) treatment decreased locomotor activity, increased the latency to a thermal analgesic response and induced a cataleptic and hypothermic state. These reactions are similar to the tetrad effects caused by the cannabinoid agonist WIN\u00a055,212\u20102 (3\u00a0mg\/kg, i.p.). The cannabinoid CB\n                    <jats:sub>1<\/jats:sub>\n                    receptor antagonist AM251 (10\u00a0mg\/kg, i.p.) reversed the effects of dipyrone on locomotor activity, the cataleptic response and thermal analgesia. Both AM251 (10\u00a0mg\/kg, i.p.) and the transient receptor potential vanilloid 1 (TRPV1) antagonist capsazepine (10\u00a0mg\/kg, i.p.) accentuated the reduction in body temperature caused by dipyrone. However, the CB\n                    <jats:sub>2<\/jats:sub>\n                    receptor antagonist AM630 did not alter the hypothermic response to dipyrone. These results indicate involvement of the endocannabinoid system, especially CB\n                    <jats:sub>1<\/jats:sub>\n                    receptors, in the analgesic and cataleptic effects of dipyrone, as well as hypolocomotion. However, cannabinoid receptors and TRPV1 were not involved in the hypothermic effects of dipyrone. We hypothesize that the mechanism of action of dipyrone may involve inhibition of cyclo\u2010oxygenase and fatty acid amide hydrolase, which together provide additional arachidonic acid as substrate for endocannabinoid synthesis or other related molecules. This increase in endocannabinoid availability enhances CB\n                    <jats:sub>1<\/jats:sub>\n                    receptor stimulation, contributing to the observed effects.\n                  <\/jats:p>","DOI":"10.1111\/1440-1681.12347","type":"journal-article","created":{"date-parts":[[2014,11,27]],"date-time":"2014-11-27T10:31:27Z","timestamp":1417084287000},"page":"246-255","source":"Crossref","is-referenced-by-count":39,"title":["Cannabinoid CB\n                    <sub>1<\/sub>\n                    receptors mediate the effects of dipyrone"],"prefix":"10.1111","volume":"42","author":[{"given":"Fernanda","family":"Crunfli","sequence":"first","affiliation":[{"name":"Department of Physiological Sciences Institute of Biomedical Sciences Federal University of Alfenas Alfenas MG"},{"name":"Graduate Program in Health Biosciences Federal University of Alfenas Alfenas MG"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fabiana C","family":"Vilela","sequence":"additional","affiliation":[{"name":"Graduate Program in Health Biosciences Federal University of Alfenas Alfenas MG"},{"name":"Faculty of Pharmaceutical Sciences Federal University of Alfenas Alfenas MG"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alexandre","family":"Giusti\u2010Paiva","sequence":"additional","affiliation":[{"name":"Department of Physiological Sciences Institute of Biomedical Sciences Federal University of Alfenas Alfenas MG"},{"name":"Graduate Program in Health Biosciences Federal University of Alfenas Alfenas MG"},{"name":"Multicenter Graduate Program in Physiological Sciences Brazilian Society of Physiology Sao Paulo Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2015,2,11]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.bjp.0707239"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.2165\/00003088-199528030-00004"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/0304-3959(86)90161-2"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0006-8993(99)02303-3"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0065-2776(08)60430-7"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.274.33.22903"},{"key":"e_1_2_7_8_1","first-page":"71","article-title":"Lack of effect of naltrindole on the spinal synergism of morphine and non\u2010steroidal anti\u2010inflammatory drugs (NSAIDS)","volume":"60","author":"Miranda HF","year":"2009","journal-title":"J. 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