{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,23]],"date-time":"2026-10-23T20:23:09Z","timestamp":1792786989042,"version":"4.5.0"},"reference-count":25,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2015,4,23]],"date-time":"2015-04-23T00:00:00Z","timestamp":1429747200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"A project supported by research fund of Guangdong provincial scientific department.","award":["2013B021800070"],"award-info":[{"award-number":["2013B021800070"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Clin Exp Pharma Physio"],"published-print":{"date-parts":[[2015,5]]},"abstract":"<jats:title>Summary<\/jats:title>\n                  <jats:p>\n                    <jats:list>\n                      <jats:list-item>\n                        <jats:p>\n                          Degeneration of the intervertebral disc (\n                          <jats:styled-content style=\"fixed-case\">IVD<\/jats:styled-content>\n                          ) is a major chronic medical condition associated with back pain. To better understand the pathogenesis of\n                          <jats:styled-content style=\"fixed-case\">IVD<\/jats:styled-content>\n                          degeneration, we performed comparative and quantitative proteomic analyses of normal and degenerated human annulus fibrosus (\n                          <jats:styled-content style=\"fixed-case\">AF<\/jats:styled-content>\n                          ) cells and identified proteins that are differentially expressed between them. Annulus fibrosus cells were isolated and cultured from patients with lumbar disc herniation (the experimental group, degenerated\n                          <jats:styled-content style=\"fixed-case\">AF<\/jats:styled-content>\n                          cells) and scoliosis patients who underwent orthopaedic surgery (the control group, normal\n                          <jats:styled-content style=\"fixed-case\">AF<\/jats:styled-content>\n                          cells). Comparative proteomic analyses of normal and degenerated cultured\n                          <jats:styled-content style=\"fixed-case\">AF<\/jats:styled-content>\n                          cells were carried out using 2\u2010D electrophoresis, mass spectrometric analyses, and database searching. Quantitative analyses of silver\u2010stained 2\u2010D electrophoresis gels of normal and degenerated cultured\n                          <jats:styled-content style=\"fixed-case\">AF<\/jats:styled-content>\n                          cells identified 10 protein spots that showed the most altered differential expression levels between the two groups. Among these, three proteins were decreased, including heat shock cognate 71\u2010\n                          <jats:styled-content style=\"fixed-case\">kD<\/jats:styled-content>\n                          a protein, glucose\u20106\u2010phosphate 1\u2010dehydrogenase, and protocadherin\u201023, whereas seven proteins were increased, including guanine nucleotide\u2010binding protein G(i) subunit\n                          <jats:italic>\u03b1<\/jats:italic>\n                          \u20102, superoxide dismutase, transmembrane protein 51, adenosine receptor A3, 26S protease regulatory subunit 8, lipid phosphate phosphatase\u2010related protein, and fatty acyl\u2010crotonic acid reductase 1. These differentially expressed proteins might be involved in the pathophysiological process of\n                          <jats:styled-content style=\"fixed-case\">IVD<\/jats:styled-content>\n                          degeneration and have potential values as biomarkers of the degeneration of\n                          <jats:styled-content style=\"fixed-case\">IVD<\/jats:styled-content>\n                          .\n                        <\/jats:p>\n                      <\/jats:list-item>\n                    <\/jats:list>\n                  <\/jats:p>","DOI":"10.1111\/1440-1681.12386","type":"journal-article","created":{"date-parts":[[2015,3,4]],"date-time":"2015-03-04T10:02:07Z","timestamp":1425463327000},"page":"530-536","source":"Crossref","is-referenced-by-count":22,"title":["Comparative and quantitative proteomic analysis of normal and degenerated human annulus fibrosus cells"],"prefix":"10.1111","volume":"42","author":[{"given":"Dongping","family":"Ye","sequence":"first","affiliation":[{"name":"The Fourth Affiliated Hospital of the Medical College Jinan University Guangzhou Institute of Traumatic Surgery Guangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Weiguo","family":"Liang","sequence":"additional","affiliation":[{"name":"The Fourth Affiliated Hospital of the Medical College Jinan University Guangzhou Institute of Traumatic Surgery Guangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Libing","family":"Dai","sequence":"additional","affiliation":[{"name":"The Fourth Affiliated Hospital of the Medical College Jinan University Guangzhou Institute of Traumatic Surgery Guangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Longqiang","family":"Zhou","sequence":"additional","affiliation":[{"name":"The Fourth Affiliated Hospital of the Medical College Jinan University Guangzhou Institute of Traumatic Surgery Guangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yicun","family":"Yao","sequence":"additional","affiliation":[{"name":"The Fourth Affiliated Hospital of the Medical College Jinan University Guangzhou Institute of Traumatic Surgery Guangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xin","family":"Zhong","sequence":"additional","affiliation":[{"name":"The Fourth Affiliated Hospital of the Medical College Jinan University Guangzhou Institute of Traumatic Surgery Guangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Honghui","family":"Chen","sequence":"additional","affiliation":[{"name":"The Fourth Affiliated Hospital of the Medical College Jinan University Guangzhou Institute of Traumatic Surgery Guangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jiake","family":"Xu","sequence":"additional","affiliation":[{"name":"School of Pathology and Laboratory Medicine University of Western Australia Perth WA Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2015,4,23]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.mod.2012.07.003"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1586\/ern.10.187"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1021\/bm3007727"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.2106\/JBJS.F.00019"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.1097\/BRS.0b013e31818f8c02"},{"key":"e_1_2_6_7_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00586-006-0125-9"},{"key":"e_1_2_6_8_1","doi-asserted-by":"publisher","DOI":"10.1097\/BRS.0b013e31818e5ddd"},{"key":"e_1_2_6_9_1","doi-asserted-by":"publisher","DOI":"10.1186\/ar2929"},{"key":"e_1_2_6_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.joca.2009.09.009"},{"key":"e_1_2_6_11_1","doi-asserted-by":"publisher","DOI":"10.1097\/BRS.0b013e31805b835d"},{"key":"e_1_2_6_12_1","doi-asserted-by":"publisher","DOI":"10.1186\/ar4274"},{"key":"e_1_2_6_13_1","doi-asserted-by":"publisher","DOI":"10.1097\/BRS.0b013e31826b71f4"},{"key":"e_1_2_6_14_1","first-page":"1567","article-title":"Expression of soluble Fas and soluble FasL in human nucleus pulposus cells","volume":"6","author":"Sun Z","year":"2013","journal-title":"Int. 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