{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,31]],"date-time":"2025-10-31T14:13:42Z","timestamp":1761920022111},"reference-count":44,"publisher":"Wiley","issue":"11","license":[{"start":{"date-parts":[[2015,10,6]],"date-time":"2015-10-06T00:00:00Z","timestamp":1444089600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Clin Exp Pharma Physio"],"published-print":{"date-parts":[[2015,11]]},"abstract":"<jats:title>Summary<\/jats:title><jats:p>Preeclampsia (PE) is one of the most common hypertensive disorders and is a leading cause of morbidity and mortality for pregnant women and perinatal babies. Additionally, pleckstrin homology\u2010like domain family A member 2 (<jats:italic>PHLDA2<\/jats:italic>) is associated with placental dysfunction. However, the effect of PHLDA2 on trophoblast cell proliferation, migration and invasion has not been investigated. In this study, 15 PE patients and 15 normal pregnant women were recruited and clinical characteristics were summarized. Pleckstrin homology\u2010like domain family A member 2 levels in placental tissues were examined using real\u2010time PCR and western blot. Overexpression plasmid and <jats:italic>PHLDA2<\/jats:italic> siRNA was introduced into JEG\u20103 cells, respectively. Cell proliferation was measured using MTT assay and flow cytometry. Cell migration and invasion capacities were assessed by wound healing and Transwell assays. It was found that PE patients collectively presented proteinuria, elevated systolic blood pressure (SBP) and diastolic blood pressure (DBP), and lower gestational ages and birth weights. Pleckstrin homology\u2010like domain family A member 2 levels in the preeclamptic placenta were significantly upregulated. Pleckstrin homology\u2010like domain family A member 2 overexpression significantly arrested cells in the G0\/G1 phase, inhibited cell proliferation and suppressed the migration and invasion of JEG\u20103 cells. Pleckstrin homology\u2010like domain family A member 2 knockdown significantly blocked the cells in the S phase of the cell cycle. Knockdown of <jats:italic>PHLDA2<\/jats:italic> alleviated the inhibition on the migration and invasion of trophoblast cells JEG\u20103. These findings illustrate that <jats:italic>PHLDA2<\/jats:italic> may participate in PE pathogenesis and indicate its potential application in the early diagnosis of PE.<\/jats:p>","DOI":"10.1111\/1440-1681.12468","type":"journal-article","created":{"date-parts":[[2015,7,28]],"date-time":"2015-07-28T14:21:02Z","timestamp":1438093262000},"page":"1142-1151","source":"Crossref","is-referenced-by-count":8,"title":["The expression of the imprinted gene pleckstrin homology\u2010like domain family A member 2 in placental tissues of preeclampsia and its effects on the proliferation, migration and invasion of trophoblast cells JEG\u20103"],"prefix":"10.1111","volume":"42","author":[{"given":"Feng","family":"Jin","sequence":"first","affiliation":[{"name":"Department of Obstetrics and Gynaecology Shengjing Hospital of China Medical University Shenyang China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chong","family":"Qiao","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynaecology Shengjing Hospital of China Medical University Shenyang China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nannan","family":"Luan","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynaecology Shengjing Hospital of China Medical University Shenyang China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tao","family":"Shang","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynaecology Shengjing Hospital of China Medical University Shenyang China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2015,10,6]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.1111726"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(05)17987-2"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1093\/jn\/133.5.1684S"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.1053\/j.semperi.2009.02.010"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.1155\/2014\/297397"},{"key":"e_1_2_6_7_1","doi-asserted-by":"publisher","DOI":"10.1136\/bmj.332.7539.463"},{"key":"e_1_2_6_8_1","first-page":"2889","article-title":"Risk for recurrence of pre\u2010eclampsia in the subsequent pregnancy","volume":"7","author":"Surapaneni T","year":"2013","journal-title":"J. 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