{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,1]],"date-time":"2025-10-01T16:30:52Z","timestamp":1759336252540},"reference-count":33,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2016,3,16]],"date-time":"2016-03-16T00:00:00Z","timestamp":1458086400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100000925","name":"National Health and Medical Research Council","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100000925","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Clin Exp Pharma Physio"],"published-print":{"date-parts":[[2016,4]]},"abstract":"<jats:title>Summary<\/jats:title><jats:p>The ghrelin gene is expressed in the stomach where it ultimately encodes up to three peptides, namely, acylated ghrelin, des\u2010acylated ghrelin and obestatin, which all have neuroendocrine roles. Recently, the authors' reported that these peptides have important physiological roles in positively regulating vasodilator nitric oxide (NO) production in the cerebral circulation, and may normally suppress superoxide production by the pro\u2010oxidant enzyme, Nox2\u2010NADPH oxidase. To date, the majority of studies using exogenous peptides infer that they may have similar roles in the systemic circulation. Therefore, this study examined whether exogenous and endogenous ghrelin\u2010related peptides modulate NO production and superoxide levels in mouse mesenteric arteries and\/or thoracic aorta. Using wire myography, it was found that application of exogenous acylated ghrelin, des\u2010acylated ghrelin or obestatin to mouse thoracic aorta or mesenteric arteries failed to elicit a vasorelaxation response, whereas all three peptides elicited vasorelaxation responses of rat thoracic aorta. Also, none of the peptides modulated mouse aortic superoxide levels as measured by L\u2010012\u2010enhanced chemiluminescence. Next, it was found that NO bioactivity and superoxide levels were unaffected in the thoracic aorta from <jats:italic>ghrelin<\/jats:italic>\u2010deficient mice when compared with wild\u2010type mice. Lastly, using novel GHSR\u2010eGFP reporter mice in combination with double\u2010labelled immunofluorescence, no evidence was found for the growth hormone secretagogue receptor (GHSR1a) in the throracic aorta, which is the only functional ghrelin receptor identified to date. Collectively these findings demonstrate that, in contrast to systemic vessels of other species (e.g. rat and human) and mouse cerebral vessels, ghrelin\u2010related peptides do not modulate vasodilator NO production or superoxide levels in mouse systemic arteries.<\/jats:p>","DOI":"10.1111\/1440-1681.12548","type":"journal-article","created":{"date-parts":[[2016,1,22]],"date-time":"2016-01-22T18:09:07Z","timestamp":1453486147000},"page":"468-475","source":"Crossref","is-referenced-by-count":3,"title":["Ghrelin\u2010related peptides do not modulate vasodilator nitric oxide production or superoxide levels in mouse systemic arteries"],"prefix":"10.1111","volume":"43","author":[{"given":"Jacqueline M","family":"Ku","sequence":"first","affiliation":[{"name":"Vascular Biology &amp; Immunopharmacology Group Department of Pharmacology Monash University Melbourne Victoria Australia"},{"name":"School of Health and Biomedical Sciences RMIT University Melbourne Victoria Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mark W","family":"Sleeman","sequence":"additional","affiliation":[{"name":"Department of Physiology Monash University Melbourne Victoria Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Christopher G","family":"Sobey","sequence":"additional","affiliation":[{"name":"Department of Physiology Monash University Melbourne Victoria Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zane B","family":"Andrews","sequence":"additional","affiliation":[{"name":"Department of Physiology Monash University Melbourne Victoria Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alyson A","family":"Miller","sequence":"additional","affiliation":[{"name":"School of Health and Biomedical Sciences RMIT University Melbourne Victoria Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2016,3,16]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1038\/45230"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0800708105"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2008.01.017"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.1152\/physrev.00012.2004"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.273.5277.974"},{"key":"e_1_2_6_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biopsych.2012.03.010"},{"key":"e_1_2_6_8_1","doi-asserted-by":"publisher","DOI":"10.1124\/pr.113.008433"},{"key":"e_1_2_6_9_1","doi-asserted-by":"publisher","DOI":"10.1530\/EJE-12-0456"},{"key":"e_1_2_6_10_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.1117255"},{"key":"e_1_2_6_11_1","doi-asserted-by":"publisher","DOI":"10.1677\/JME-09-0141"},{"key":"e_1_2_6_12_1","doi-asserted-by":"publisher","DOI":"10.1210\/en.2006-0933"},{"key":"e_1_2_6_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tins.2010.10.001"},{"key":"e_1_2_6_14_1","doi-asserted-by":"publisher","DOI":"10.1258\/ebm.2011.010365"},{"key":"e_1_2_6_15_1","doi-asserted-by":"publisher","DOI":"10.1210\/en.2014-1415"},{"key":"e_1_2_6_16_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1476-5381.2011.01761.x"},{"key":"e_1_2_6_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ejphar.2008.10.032"},{"key":"e_1_2_6_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cardiores.2005.09.001"},{"key":"e_1_2_6_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbrc.2012.10.137"},{"key":"e_1_2_6_20_1","doi-asserted-by":"publisher","DOI":"10.1210\/en.2008-0255"},{"key":"e_1_2_6_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.amjhyper.2006.01.022"},{"key":"e_1_2_6_22_1","first-page":"3023","article-title":"Ghrelin restores nitric oxide availability in resistance circulation of essential hypertensive patients: Role of NAD(P)H oxidase","volume":"36","author":"Virdis A","year":"2015","journal-title":"Eur. 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