{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,21]],"date-time":"2025-10-21T03:25:40Z","timestamp":1761017140300},"reference-count":19,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2016,4,7]],"date-time":"2016-04-07T00:00:00Z","timestamp":1459987200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["31171108"],"award-info":[{"award-number":["31171108"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Clin Exp Pharma Physio"],"published-print":{"date-parts":[[2016,5]]},"abstract":"<jats:title>Summary<\/jats:title><jats:p>Hydrogen sulphide (H<jats:sub>2<\/jats:sub>S) is generated endogenously from <jats:sc>l<\/jats:sc>\u2010cysteine (<jats:sc>l<\/jats:sc>\u2010Cys) by the enzymes cystathionine\u2010<jats:italic>\u03b2<\/jats:italic>\u2010synthase (<jats:styled-content style=\"fixed-case\">CBS<\/jats:styled-content>) and cystathionine\u2010<jats:italic>\u03b3<\/jats:italic>\u2010lyase (<jats:styled-content style=\"fixed-case\">CSE<\/jats:styled-content>). In addition, <jats:sc>l<\/jats:sc>\u2010Cys is commonly used as a precursor in the food and pharmaceutical industries. The aim of the present study is to determine whether <jats:sc>l<\/jats:sc>\u2010Cys regulates intestinal nutrient transport. To that end, the presence of <jats:styled-content style=\"fixed-case\">CBS<\/jats:styled-content> and <jats:styled-content style=\"fixed-case\">CSE<\/jats:styled-content> in the jejunum epithelium was assessed by immunohistochemistry, Western blotting and the methylene blue assay. In addition, <jats:italic>in\u00a0vivo <\/jats:italic><jats:sc>l<\/jats:sc>\u2010Cys (100\u00a0mg\/kg, administered immediately after the glucose load) significantly increased blood glucose levels 30\u00a0min after the oral administration of glucose to mice. This effect of <jats:sc>l<\/jats:sc>\u2010Cys was completely blocked by amino\u2010oxyacetic acid (<jats:styled-content style=\"fixed-case\">AOA<\/jats:styled-content>; 50\u00a0mg\/kg; administered at the same time as <jats:sc>l<\/jats:sc>\u2010Cys) an inhibitor of <jats:styled-content style=\"fixed-case\">CBS<\/jats:styled-content>. Measurements of the short\u2010circuit current (<jats:italic>I<\/jats:italic><jats:sub>sc<\/jats:sub>) in the rat jejunum epithelium revealed that <jats:sc>l<\/jats:sc>\u2010Cys (1\u00a0mmol\/L; 6\u00a0min before the administration of <jats:sc>l<\/jats:sc>\u2010alanine) enhances Na<jats:sup>+<\/jats:sup>\u2010coupled <jats:sc>l<\/jats:sc>\u2010alanine or glucose transport, and that this effect is inhibited by <jats:styled-content style=\"fixed-case\">AOA<\/jats:styled-content> (1\u00a0mmol\/L;10\u00a0min before the administration of <jats:sc>l<\/jats:sc>\u2010Cys), but not <jats:sc>d<\/jats:sc>,<jats:sc>l<\/jats:sc>\u2010propargylglycine (<jats:styled-content style=\"fixed-case\">PAG<\/jats:styled-content>;1\u00a0mmol\/L; 10\u00a0min before the administration of <jats:sc>l<\/jats:sc>\u2010Cys), a <jats:styled-content style=\"fixed-case\">CSE<\/jats:styled-content> inhibitor. Notably, <jats:sc>l<\/jats:sc>\u2010Cys\u2010evoked enhancement of nutrient transport was alleviated by glibenclamide (Gli;0.1\u00a0mmol\/L; 10\u00a0min before the administration of <jats:sc>l<\/jats:sc>\u2010Cys), a K<jats:sup>+<\/jats:sup> channel blocker. Together, the data indicate that <jats:sc>l<\/jats:sc>\u2010Cys enhances jejunal nutrient transport, suggesting a new approach to future treatment of nutrition\u2010related maladies, including a range of serious health consequences linked to undernutrition.<\/jats:p>","DOI":"10.1111\/1440-1681.12562","type":"journal-article","created":{"date-parts":[[2016,2,22]],"date-time":"2016-02-22T15:50:42Z","timestamp":1456156242000},"page":"562-568","source":"Crossref","is-referenced-by-count":9,"title":["<scp>l<\/scp>\u2010Cysteine enhances nutrient absorption via a cystathionine\u2010<i>\u03b2<\/i>\u2010synthase\u2010derived H<sub>2<\/sub>S pathway in rodent jejunum"],"prefix":"10.1111","volume":"43","author":[{"given":"Ailin","family":"Xiao","sequence":"first","affiliation":[{"name":"Department of Physiology School of Medicine Shandong University Jinan China"},{"name":"Department of Medical Foundation Hubei College of Chinese Medicine Jingzhou China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jing","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Pathology Central Hospital of Zibo Zibo China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tianjian","family":"Liu","sequence":"additional","affiliation":[{"name":"Department of Physiology School of Medicine Shandong University Jinan China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zhuxi","family":"Liu","sequence":"additional","affiliation":[{"name":"Department of Physiology School of Medicine Shandong University Jinan China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chuanfei","family":"Wei","sequence":"additional","affiliation":[{"name":"Department of Physiology School of Medicine Shandong University Jinan China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xiaomeng","family":"Xu","sequence":"additional","affiliation":[{"name":"Department of Physiology School of Medicine Shandong University Jinan China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Qin","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Physiology School of Medicine Shandong University Jinan China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jingxin","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Physiology School of Medicine Shandong University Jinan China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2016,4,7]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1096\/fj.02-0211hyp"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.dld.2009.05.016"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1089\/ars.2013.5312"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1124\/jpet.105.091595"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1053\/j.gastro.2006.08.035"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1476-5381.2009.00385.x"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1097\/00075197-200201000-00014"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1152\/physrev.1998.78.4.969"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1017\/S0029665111000103"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpcell.1986.250.1.C138"},{"key":"e_1_2_7_12_1","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1152\/physrev.1992.72.1.1","article-title":"Potassium channels in renal epithelial transport regulation","volume":"72","author":"Lang F","year":"1992","journal-title":"Physiol. 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